FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003652764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003652764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA291.9320453031312457Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG150.9993337774816788Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT130.866089273817455Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC130.866089273817455Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC80.5329780146568954Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG70.4663557628247834Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT50.3331112591605596Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC50.3331112591605596Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG50.3331112591605596Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG50.3331112591605596Clontech Universal Primer Mix Long (96% over 26bp)
CACTGGCCCAGTCCGGCCTGCCATTGGCCATCGCGAGCGCCGCCATGCTC40.2664890073284477No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC40.2664890073284477Clontech SMART CDS Primer II A (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG40.2664890073284477Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG40.2664890073284477Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.2664890073284477No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.2664890073284477No Hit
TCCCAGCCCCAAAGCTGAAGACCTTGTTCAAAGTCCGATGTTCAAAGGCA30.19986675549633579No Hit
GGATTAAGGCGACAGCGATTTCTAGGATAGTCAGTAGAATTAGAATTGTG30.19986675549633579No Hit
CCACGCGCTTCACGCCATGCTCCGAGAGCCAGGCTTCCAGCGGCGTGCGC30.19986675549633579No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT30.19986675549633579Clontech Universal Primer Mix Long (100% over 23bp)
GAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.19986675549633579Clontech SMARTer II A Oligonucleotide (95% over 21bp)
ATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAA30.19986675549633579No Hit
GACAGAGTCTTGTTCTGTCTCCCAGGCTGGAGTGCAATGGCGCAATCTCG30.19986675549633579No Hit
CTCATCGAGCGCGGTCTCCCATGAGACCTGTTCGAACTCGCCGTCCGGGC30.19986675549633579No Hit
TCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGT30.19986675549633579No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA30.19986675549633579Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCCGGCACCGAGGTGGTAGCGCAGGCGGAGATCGGCGGTCTGCCCGCCG30.19986675549633579No Hit
CTTGTGGGTATGTAGTTGCTTGATTTTTTTTTTATTATTAACTCTGATAG30.19986675549633579No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC30.19986675549633579Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTG30.19986675549633579Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC30.19986675549633579Clontech Universal Primer Mix Long (96% over 26bp)
CTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGATAATTGCG30.19986675549633579No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCCGACCGAGAT30.19986675549633579Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTT30.19986675549633579No Hit
GAGCCACAGTGATATCAGATGGAACCAAGTACTTCCGTTTGTCAATGTCA30.19986675549633579No Hit
CTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCG30.19986675549633579No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG20.13324450366422386Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC20.13324450366422386Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAA20.13324450366422386Clontech Universal Primer Mix Long (95% over 22bp)
ATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG20.13324450366422386No Hit
GCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATT20.13324450366422386No Hit
GAAGGTTCCAACCTCTTCATACGTTGCTGGTGGGAATATAAAATGGTGCA20.13324450366422386No Hit
CAAACACACTGATTCAAGTTTTGACCTGTTGTGGCTACTAACTTCTCCCA20.13324450366422386No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA20.13324450366422386Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA20.13324450366422386Clontech Universal Primer Mix Long (95% over 21bp)
GGCGGGGACAGGCGGGGGACCGGCTATCCGAGGCCAACCGAGGCTCCGCG20.13324450366422386No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC20.13324450366422386No Hit
AGCTTACATTTACCCAAACTGTCCATTACTGGAACCTATGATCTGAAGAG20.13324450366422386No Hit
TCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTCTAG20.13324450366422386No Hit
GCAGAGTACATGGGTGACGGGACCACCAGTCTACATCTTTCCCTACACGA20.13324450366422386No Hit
GTTAAAGGCCAAGATGTCGATTTTAAAAAATAATCTCAAAAATAATTGTA20.13324450366422386No Hit
ATCAACGCAGAGTACATGGGTGATACGGCGACACCGAGATCTACACTCTT20.13324450366422386No Hit
GTGCGGCGAATTCGGCCTCAACTTCATCGTTCGACCGCCCCAATCCAGCG20.13324450366422386No Hit
ACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCG20.13324450366422386No Hit
TCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTC20.13324450366422386No Hit
GGTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAGAGTACATGGG20.13324450366422386Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ATCGTGCGTAGGTACTCTTCAATGCAGCGTGGACGAGACGACGTCACATA20.13324450366422386No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT20.13324450366422386Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA20.13324450366422386No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA20.13324450366422386No Hit
CTCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGCGGTACACCTGTCA20.13324450366422386No Hit
AGAAAGCCTTGTGACTTAGGAATTAGAAATCAGTTTCTTTGTGAAATGCC20.13324450366422386No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT20.13324450366422386Clontech Universal Primer Mix Long (96% over 26bp)
TTTTTACATATCTCATTGAGGCTAATTTTACATGGGAGGAAACTGAGAAT20.13324450366422386No Hit
GTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGA20.13324450366422386No Hit
GTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACACCTGTCTCTT20.13324450366422386No Hit
CGTATGAACGTTGCTGAAGTGGTAATTGAGGAAAACAGTTCCCCAGATTG20.13324450366422386No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC20.13324450366422386Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATAACTGTGGTATTTCTAG20.13324450366422386No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTACGGCCGA20.13324450366422386Clontech Universal Primer Mix Long (96% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.13324450366422386No Hit
CTCAAAAAAGAAAGAAAAAAATGTAGATGAATACGTATCACAAAGTATCA20.13324450366422386No Hit
GTAGATTACTGTATTGGTTTTACCAAAAACTTGGCGACTTAAAATAACAC20.13324450366422386No Hit
TGTCCGGGCCGGGTGAGGTTTCCCGTGTTGGGTCAAATTAAGCCGCAGGC20.13324450366422386No Hit
GTCTCACCCTGTCACCCAGGCTGGGATGCCCAGTGGCATGATCTCAGCTC20.13324450366422386No Hit
CGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATG20.13324450366422386No Hit
GGTGACGTCCGCAATGCGTCGCCAAGCGAAAGCCGTCAACTTCGGTATCG20.13324450366422386No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTGAGACAGGGTCTCACTTTG20.13324450366422386No Hit
GTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCA20.13324450366422386No Hit
GACTTATAATGGAGTTGATAACAACAAGAATAAAGGGCAGCTGACTAAGA20.13324450366422386No Hit
GCCAGCCATGGGCGGCCAGTCCGCGCCCGGCACCGACGGGACGTCCGGAG20.13324450366422386No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCAC20.13324450366422386No Hit
CCGGCGGGGACAGGCGGGGGACCGGCTATCCGAGGCCAACCGAGGCTCCG20.13324450366422386No Hit
TATCAACGCAGAGTACTGGGAATGGAATTAAAAGCCTCCTATCTCTACCC20.13324450366422386No Hit
GGGGTCGCCTTCCCGGCCGACCCGGCCTAACAGCTCGGGCTCGCCGCCAA20.13324450366422386No Hit
CCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGG20.13324450366422386No Hit
ATGCTAGCCAGGATGTTTTTGATCTCCTGACCTTGTAATCTGCCCGCCTC20.13324450366422386No Hit
GTGAGCCATGGAGAACCATCAGCTTTCATGGGTGCTTCAGCCTCTGTTTT20.13324450366422386No Hit
GGTATGAAGGAGCTGGCCGTGTTCCGGGAGAAGGTCACTGAGCAGCACCG20.13324450366422386No Hit
GAATTAACATCTAAAAACAAGGAAAAGACTCAAGGGGACATTTTTTTGGT20.13324450366422386No Hit
TATCTCTGGACTTCTCCCTCTAAAACCCAGTGGCCCAAAGAGCTGAAAAA20.13324450366422386No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTCTGTCTCTT20.13324450366422386Clontech Universal Primer Mix Long (96% over 26bp)
GTTACTTTCAACTCTTAGTGCCGGGTCGCCACTTAACTATTTGTGTGACC20.13324450366422386No Hit
TATCAACGCAGAGTACATGGGTGACGACCACCAATCTACCTCTTTCCCTA20.13324450366422386No Hit
CTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTC20.13324450366422386No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC20.13324450366422386No Hit
CTATGAACGCTTGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCT20.13324450366422386No Hit
GAGTGAGACCCTGTCTCAGGAAAGAAACAAACAAACACAATTTGGGGTGC20.13324450366422386No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC20.13324450366422386No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT20.13324450366422386Clontech Universal Primer Mix Long (96% over 26bp)
GATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATG20.13324450366422386No Hit
ACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAA20.13324450366422386No Hit
GTCTTTGACCGTCGTCACGACCGCGTCGAGCCGCTGCTCGTCCGCCCAGA20.13324450366422386No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.13324450366422386Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGAGCGTCGTGTAGGGAAAGAGTGTAGACTCGGTGGTCGCGTAATCC20.13324450366422386Illumina Single End PCR Primer 1 (96% over 31bp)
AAAAAAGACACATGCACTCATATGTTCATTGCAGCATTATTCACGATAAC20.13324450366422386No Hit
CTATGAAAACGTGCAAATGTATTGTAGAGACTTTATGATTAGAATTGCAT20.13324450366422386No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT20.13324450366422386No Hit
GCGCCATGGCCGACGGCGAGGCGGAGGCCGGCGAGCAGCAGCTGATCATG20.13324450366422386No Hit
GTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTG20.13324450366422386No Hit
AAAGAATGTCTTCGATAGTTCTGTTAGATATATATTTGTATAAAATGTAT20.13324450366422386No Hit
CCCAAGAAACATATATCCCAGATTTCTGTGGCAGAAGATGATGATGAAAG20.13324450366422386No Hit
TACGTAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACT20.13324450366422386No Hit
GTATGAGAAAACAGAAGTGAATTCTGGCTTTTTTTACAAGAGTGCAGAAG20.13324450366422386No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTC20.13324450366422386Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGGCACTTCCCTACACGACGCTCTTCCGATCTATCCCTTTT20.13324450366422386Illumina Single End PCR Primer 1 (96% over 27bp)
GAGAGTAGGCTGCCATGAGGAGGTAAGCCAGTAAGGGTGCCTCGCTTTGG20.13324450366422386No Hit
CGTGCGGATAGGGCAGGTCGGGAAGCGAGAAGGCCATGCGCGGCTCCTGT20.13324450366422386No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTAT20.13324450366422386Clontech Universal Primer Mix Long (95% over 21bp)
TCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCT20.13324450366422386No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA20.13324450366422386Clontech Universal Primer Mix Long (96% over 26bp)
GCTCGGACCTAGGTCGCGGCGACATGGCCAAACGTACCAAGAAAGTCGGG20.13324450366422386No Hit
CAGCCCCTCTCCGGCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGG20.13324450366422386No Hit
ATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAG20.13324450366422386No Hit
TCAGAAACACGTGCACTTTATTGAATGCCATTGTAGAAAAGCGTGTGAGG20.13324450366422386No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTGCTTCCCATGTACTCTGC20.13324450366422386Clontech SMART CDS Primer II A (96% over 26bp)
CGGTCACAGAGCCCAGCTTCTCCGTGTTAATACTCGTTAACAGGGAGATC20.13324450366422386No Hit
GGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTT20.13324450366422386No Hit
ACCGAGATCTACATCTTTCCCTACACGACGCTCTTCCGATCTCTGGTACC20.13324450366422386Illumina Single End PCR Primer 1 (100% over 29bp)
ATTTGACTCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTG20.13324450366422386No Hit
GTGGTGGTGGTTGTTGGCTTTCTTGGAGAAATATATCTGTGACTTTCTCA20.13324450366422386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.13324450366422386No Hit
CTCCCAGCCTAGGCCCTTAATCTTGCTGTTATTTTCCATGGACTAAAGGT20.13324450366422386No Hit
GTCCTCGATCCGCTCGGCCTCTAATCCCTGCCCTGGCCTGCGCGGGCCCG20.13324450366422386No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTT20.13324450366422386Clontech Universal Primer Mix Long (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)