FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003652765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003652765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1029
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA474.56754130223518Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT353.4013605442176873Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG201.9436345966958213Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG201.9436345966958213Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG171.6520894071914478Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC151.4577259475218658Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC141.3605442176870748Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT131.2633624878522838Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA111.0689990281827018Clontech Universal Primer Mix Long (95% over 21bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC111.0689990281827018Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC90.8746355685131195Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG80.7774538386783284Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC80.7774538386783284Clontech Universal Primer Mix Long (96% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA80.7774538386783284Clontech SMART CDS Primer II A (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT70.6802721088435374Clontech Universal Primer Mix Long (100% over 23bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT70.6802721088435374Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC60.5830903790087464Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG60.5830903790087464Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.5830903790087464Clontech Universal Primer Mix Long (95% over 22bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA60.5830903790087464Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG50.4859086491739553Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC50.4859086491739553Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC50.4859086491739553Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC40.3887269193391642Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA40.3887269193391642Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT40.3887269193391642Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT40.3887269193391642Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCGTTAACACCCCATTCGGAATCCAGCGCGTTGACACGCACAATGGTTT30.2915451895043732No Hit
CGCTATGCGCTGTCGCAGCACCAGCTCATCCGGCGCAGCCTCTCGGACCA30.2915451895043732No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA30.2915451895043732Clontech Universal Primer Mix Long (96% over 25bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC30.2915451895043732Clontech Universal Primer Mix Long (96% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA30.2915451895043732Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA30.2915451895043732Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT30.2915451895043732Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCACTGCTTCCCATGTACTCTGC20.1943634596695821No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG20.1943634596695821Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG20.1943634596695821Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACGCAGATACCACTGCTTCCCATGTACTCTGCGTCTGTCTCTTATACA20.1943634596695821No Hit
GTAGCCATGAGGGTCCTCGCCCGCTGGAATAATTGTTTCAACCGTCACTT20.1943634596695821No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG20.1943634596695821Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA20.1943634596695821Clontech Universal Primer Mix Long (95% over 22bp)
TACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT20.1943634596695821Clontech Universal Primer Mix Long (96% over 26bp)
CTTGCCTCAGATTCTGTCGGTACCAGAGATCGGAAGAGCGTCGTGTAGGG20.1943634596695821Illumina Single End PCR Primer 1 (100% over 24bp)
TCCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA20.1943634596695821Clontech Universal Primer Mix Long (96% over 26bp)
GCGTGAATCTGGCGGGACCACCCGTCAAGCCTAAATACTCCTTGGTGACC20.1943634596695821No Hit
GCGCCTACACCCCGTACATCGTGGTGATGGCGCGCGACGGGGCTTCTCCG20.1943634596695821No Hit
GTGATCATGAGGCTTGTGAAGCACTCAGCAGAATAAGGGGCTCCCACGGT20.1943634596695821No Hit
GTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGAGTACATGG20.1943634596695821Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA20.1943634596695821Clontech SMART CDS Primer II A (96% over 26bp)
GTGCGTCGAAGTCCGGTGGCAGCGGCCCGGTGGTAATGACGCCACTGACC20.1943634596695821No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATAC20.1943634596695821Clontech SMART CDS Primer II A (100% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTG20.1943634596695821Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG20.1943634596695821No Hit
GGTGCCAGGCGTCCGGCTACTTCACTCATCGGGGCGAGGAGGGGCAGTGA20.1943634596695821No Hit
CCCATGTACTCTGCGTTGATACTACTGCTTCCCATGTACTCTGCGTTGAT20.1943634596695821Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT20.1943634596695821No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATA20.1943634596695821Clontech Universal Primer Mix Long (96% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGT20.1943634596695821Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGG20.1943634596695821Clontech SMART CDS Primer II A (100% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.1943634596695821Clontech Universal Primer Mix Long (96% over 26bp)
GCCAATCTCAAGTAGCAACACAACAACAACCTGTACAAGTATCAGCACCA20.1943634596695821No Hit
GCGCACCTCGGCGTGCGGCGCGCCGGACAGCCGGGCGGCGGCGCGCTCGA20.1943634596695821No Hit
GGGAAAGAGTGTAGATCTCGGTGGTCCCGTATCATCCCATGTACCTGTCT20.1943634596695821Illumina Single End PCR Primer 1 (96% over 28bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT20.1943634596695821Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCCTGTCTCTTAT20.1943634596695821No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA20.1943634596695821Clontech Universal Primer Mix Long (95% over 21bp)
GAGGTAGACTGTGGTCGTTCCCATGTACTCTGCGTTGATACCACTGCTTC20.1943634596695821Clontech SMART CDS Primer II A (96% over 26bp)
CTATGATGGATGGAAAGGAGGCCTACAATGAGAAAGTGAAAGCTTTGAAC20.1943634596695821No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAA20.1943634596695821Clontech SMART CDS Primer II A (100% over 26bp)
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC20.1943634596695821Clontech Universal Primer Mix Long (96% over 26bp)
AAACAACAAAACACTCTACATAAATCAAAATACAATAAAATCAGTAGCTA20.1943634596695821No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG20.1943634596695821Clontech SMART CDS Primer II A (96% over 26bp)
TCCCAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.1943634596695821Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGCTGTCTCTTATA20.1943634596695821Clontech Universal Primer Mix Long (96% over 26bp)
GGTCGCGTATCATCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT20.1943634596695821Clontech Universal Primer Mix Long (96% over 26bp)
AACCTAGCAAGACAGGCCAACATTCAAATTCAGGAAATACAGAGAACACC20.1943634596695821No Hit
GCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT20.1943634596695821Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCA20.1943634596695821Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT20.1943634596695821Clontech Universal Primer Mix Long (96% over 26bp)
ATCCATTTTCTATCCATGGTGCGGAGCATCATCATACGTTCGAACAGGCG20.1943634596695821No Hit
TCTACACTCTTTCCCTACACGACGCTCTTCCGATCTGTCCCAGGTCTTGT20.1943634596695821Illumina Single End PCR Primer 1 (100% over 36bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC20.1943634596695821Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG20.1943634596695821Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTCTGCAGGTACCAG20.1943634596695821Illumina Single End PCR Primer 1 (100% over 25bp)
GAGTACATGGGATGTAGCGCACCGGATCTATTTCCCTACACCACGCTCTT20.1943634596695821No Hit
CGACCAAACAGTTCACCGCTGCTGCTGAAAAGACCGTCGCTTCGGCCCGC20.1943634596695821No Hit
GAGTACATGGGAAGCAGTGGTATCTGCGTTGATACCACTGCTTCCCATGT20.1943634596695821Clontech Universal Primer Mix Long (95% over 24bp)
GTGGCGCTGCTGGTCGACGCGGTGGGCATCACGATGGTGTCGCTGACCGA20.1943634596695821No Hit
TCCCTACACGACGCTCGTTTTTCAGGACCTGTTGGTACCAGATCGGAAGA20.1943634596695821No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGATACCACTGCTTCC20.1943634596695821Clontech Universal Primer Mix Long (95% over 24bp)
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1943634596695821Clontech Universal Primer Mix Long (95% over 21bp)
CGCGAAAAGTTCAGCCATTCGCGGCTGCGGGTGGGCTCGCAACTGACCCG20.1943634596695821No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA20.1943634596695821Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCACTGGTATC20.1943634596695821Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)