FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003652766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003652766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1029
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA575.539358600583091Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT454.373177842565598Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG232.2351797862001943Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG181.749271137026239Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT171.6520894071914478Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC151.4577259475218658Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC141.3605442176870748Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG141.3605442176870748Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT111.0689990281827018Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT100.9718172983479106Clontech Universal Primer Mix Long (100% over 23bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC100.9718172983479106Clontech SMART CDS Primer II A (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG100.9718172983479106Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC80.7774538386783284Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG80.7774538386783284Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG80.7774538386783284Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC80.7774538386783284Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA70.6802721088435374Clontech Universal Primer Mix Long (95% over 21bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT70.6802721088435374Clontech SMART CDS Primer II A (100% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA60.5830903790087464Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA50.4859086491739553Clontech Universal Primer Mix Long (96% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG50.4859086491739553Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC50.4859086491739553Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT50.4859086491739553Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC50.4859086491739553Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG40.3887269193391642Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT40.3887269193391642Clontech Universal Primer Mix Long (96% over 26bp)
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAT40.3887269193391642Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA40.3887269193391642Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC30.2915451895043732Clontech Universal Primer Mix Long (96% over 26bp)
ACTCGCACCCGCCGCAGCATGTTGTTTGTGCCTGGTGCCAATGCCGCGAT30.2915451895043732No Hit
GAACAGCGCCAGCGCATGCACCGGCTGGCGCAGGTCATGGCTGGCCGAGG30.2915451895043732No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA30.2915451895043732Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT30.2915451895043732Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA30.2915451895043732Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGC30.2915451895043732Clontech Universal Primer Mix Long (96% over 26bp)
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30.2915451895043732Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC30.2915451895043732Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA30.2915451895043732Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTATAATATTGACGATTCTGAACATATTGATTATGAACAGTTCATCAATA20.1943634596695821No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG20.1943634596695821Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA20.1943634596695821Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGCTACTTGCTCGTCGACTCCGAGAAATTCCCGGGCGTCGGAGCGCATT20.1943634596695821No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAACTGTCTCTTATA20.1943634596695821Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGATCGCGCCC20.1943634596695821Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACCTG20.1943634596695821Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC20.1943634596695821Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTAT20.1943634596695821No Hit
GCCCCGCTACGGGACCGTGGTGGAGGCGGGCGCGTCCATCGGCGTGCTCA20.1943634596695821No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA20.1943634596695821Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG20.1943634596695821Clontech Universal Primer Mix Long (100% over 22bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA20.1943634596695821No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTGCTTCCCATGT20.1943634596695821Clontech Universal Primer Mix Long (96% over 26bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATAC20.1943634596695821Clontech Universal Primer Mix Long (96% over 26bp)
CACCGACACCGCGAAGCCGCCGAACACGCCCGCAGCGACGTTCGAGGCGC20.1943634596695821No Hit
GTTGTAAATGTGAATTTACATTTACTGATGAACCACTTGAAGTTTACTTG20.1943634596695821No Hit
ACTTCAACCTTAGTGAATCTGACAATTATGTGTCTTGGGGTTGCTCTTCT20.1943634596695821No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.1943634596695821Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT20.1943634596695821Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTGTGGCCCTATTCTTAGGTGCATGTGCGAGCGGTGGGGAGTCAGCTAG20.1943634596695821No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG20.1943634596695821Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGATGATACGGGACCACCGAGATCTACACTCTTTCCCCTGTCT20.1943634596695821Illumina Single End PCR Primer 1 (96% over 26bp)
CTACGAAACCGTTGTCGGTTCCGACGGTTCACTGCCCCTCCTCGCCCCGA20.1943634596695821No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTATCAACGCAGA20.1943634596695821Clontech Universal Primer Mix Long (96% over 26bp)
GTCCCAAGTTCTGCAGGTACCAGAGATCGGAAGAGCGTCGTGTAGGGAAA20.1943634596695821Illumina Single End PCR Primer 1 (100% over 27bp)
ACTCTTTCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTCTGCAGGT20.1943634596695821Illumina Single End PCR Primer 1 (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT20.1943634596695821Clontech Universal Primer Mix Long (96% over 26bp)
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC20.1943634596695821Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG20.1943634596695821Clontech Universal Primer Mix Long (96% over 26bp)
TAATTGCGGCCGAGCGCCGAAGCGGCCCGGTTTTCCGAACTCATCAGGGC20.1943634596695821No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCTGCGTTGCTGTCTCTTATACA20.1943634596695821No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAACGACCACAG20.1943634596695821Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCTTCCCGACCGAACATGTCTTTCTCGTCCAGTTCCACGTCGTCAAAGG20.1943634596695821No Hit
CTTCCTACACGACGCTCTTCCGATCTCATGATGCAAGGACTGGAGTTGCT20.1943634596695821Illumina Single End PCR Primer 1 (100% over 23bp)
CTCCCCCACCACGGGTTAACCTCGCCACGCATCACTAACTCGCAGGCTCA20.1943634596695821No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT20.1943634596695821Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT20.1943634596695821Clontech Universal Primer Mix Long (96% over 26bp)
TCCGCATCAAGCGTGACGACCACACCCTGCAGAGCCTCAACACCAAGGGC20.1943634596695821No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1943634596695821Clontech Universal Primer Mix Long (95% over 22bp)
TCCCTACACCACGCTCTTCCGATCTCTGTCCCAAGTTCTGCAGGTACCAG20.1943634596695821Illumina Single End PCR Primer 1 (96% over 25bp)
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1943634596695821No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGATACCACTGCTTCCCATGTAC20.1943634596695821Clontech Universal Primer Mix Long (95% over 21bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTAGTATCAACGCA20.1943634596695821Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCCGAGAGTACA20.1943634596695821Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCCGATCTATCCCTTTTTTGGGCCTGTTGGTACCAAAAAAGGGATAGA20.1943634596695821No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCAGTGCTTCCCATGTACTCTGC20.1943634596695821Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAGCAGACACTATGGCAGCGGCTGCTGACCGTGGGAGCCCCTTATTCTGC20.1943634596695821No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.1943634596695821No Hit
GGGAAGCAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1943634596695821Clontech Universal Primer Mix Long (95% over 24bp)
AAGCAGGGCTACCTTATATAGGAAGTTGAGTTTGAATTGAGTTTGAATCG20.1943634596695821No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT20.1943634596695821Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA20.1943634596695821Clontech Universal Primer Mix Long (96% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA20.1943634596695821Clontech SMART CDS Primer II A (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAGTACTTTT20.1943634596695821Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT20.1943634596695821Clontech SMART CDS Primer II A (100% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA20.1943634596695821Clontech SMART CDS Primer II A (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTCTCTTATA20.1943634596695821Clontech SMART CDS Primer II A (96% over 26bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20.1943634596695821Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)