FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003652777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003652777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG92.990033222591362Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG61.9933554817275747Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA41.3289036544850499Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC41.3289036544850499Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG30.9966777408637874Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30.9966777408637874Clontech Universal Primer Mix Long (96% over 26bp)
GGCCGGCACCCAGGAAGGTCGCCAGCATTGAGGTCGCACCATCGGCAGCC20.6644518272425249No Hit
GTACATGGGTAGGACCAGAGATCACACTTTCCTACACGACGCTCTTCCGG20.6644518272425249Illumina Single End Sequencing Primer (95% over 22bp)
TGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCT20.6644518272425249Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTA20.6644518272425249Illumina Single End PCR Primer 1 (100% over 21bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT20.6644518272425249Clontech Universal Primer Mix Long (96% over 26bp)
CTCTTTCCCTACACGACGCTCTTCCGATCTATCCCTTTTTTTGGTACCAA20.6644518272425249Illumina Single End PCR Primer 1 (100% over 30bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC20.6644518272425249Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG20.6644518272425249Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG20.6644518272425249Clontech Universal Primer Mix Long (96% over 26bp)
CTCTTTCCCTACACGACGCTCTTGCCTCCGATCTCTGGTACCGACAGAAT20.6644518272425249Illumina Single End PCR Primer 1 (96% over 25bp)
TGGCTGCATAGAATTCCATGGTGTATATGTATCACATTTTCTTAATCTAG20.6644518272425249No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGAGCCTGTCTCTTATACA20.6644518272425249Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGCGTCGTGTAGGGAAGAGTTAAGGTTCCCATGTACTCTGCGTTGATAC20.6644518272425249No Hit
TCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTCTGCAGGTACCAG20.6644518272425249Illumina Single End PCR Primer 1 (100% over 25bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA20.6644518272425249Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT20.6644518272425249Clontech SMART CDS Primer II A (100% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.6644518272425249No Hit
TTCATCGGTGGAATGGAAGGAGTTGAAATAGAATATAAGATGTTTCAGCA20.6644518272425249No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT20.6644518272425249Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCTGTCTCTTATACACATCTCCGAGCC20.6644518272425249No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA20.6644518272425249Clontech Universal Primer Mix Long (95% over 21bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA20.6644518272425249Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCAAGACTCTTTGTCGCACTCCTTGTTTTAACATTTCGTCACGATGAC10.33222591362126247No Hit
GTCCAGGGCAGTCTGGAGGTTGGGAAGCGTGGTGAGGCCCGCTCTGGTGG10.33222591362126247No Hit
GTGTAGCTTGCATGCCGGTAAGGCAGAGCGCCGACAGGAGCGTGTCGGCC10.33222591362126247No Hit
CTCCTGCGCAGCGACGCCCGGATCAGCCTGCCTGACGAGGCCGCTGCCCT10.33222591362126247No Hit
GATCGGTGCAAGCAAGGCAAGCTCAGCATCTTGGTCCCATCACGACCTGT10.33222591362126247No Hit
CAACATGCAAGAAAAGGAGAACCACAGAAATACACTATAAACAAACAAAA10.33222591362126247No Hit
GGCGAGCTCACGGGCGGGTGGCTGATGGAATCGGCGCCGGGGCGGCGCTT10.33222591362126247No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTAACAGCCCGATCGCGGTT10.33222591362126247No Hit
GATCTCGGTGGTCGCCGTATCATGCCATGTACTCTGCGTTGATACCCTGT10.33222591362126247Illumina Single End PCR Primer 1 (95% over 23bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTT10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAAAAA10.33222591362126247No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGAAAAAAAA10.33222591362126247Clontech Universal Primer Mix Long (96% over 25bp)
GCCCCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCCTGTCTCTTA10.33222591362126247Illumina Single End PCR Primer 1 (97% over 37bp)
GGTGCTGCCTGGGCGTTGCCAGGTGACGCGGTTGCGATCGCACTGTCGTG10.33222591362126247No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCTGGCTCT10.33222591362126247No Hit
GTACATGGGGGGCCTTTGGCGCGGCTTTCCTTGCGGCGGTTTTCGCGGGT10.33222591362126247No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTT10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTGTTAAAACAG10.33222591362126247No Hit
CTTTCCCTACACGACGCTCTTCCGATCTGTTCTGGTATCGACAAGACCTG10.33222591362126247Illumina Single End PCR Primer 1 (100% over 28bp)
CTGCGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCTGTCTCTTAT10.33222591362126247Clontech SMART CDS Primer II A (96% over 26bp)
GTATCCACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAGG10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
AATCATACAACAACTGAAGAAGATGTAAAAACTCACATAAACACATATAA10.33222591362126247No Hit
TGGGTGTACGGGACCACCGAGATCTCACTCTTTCCTACACGACGCTCTTC10.33222591362126247No Hit
GTACAGGCACATTGATATCAAATACGGCGTCATTAATTTTTGGGCTATTT10.33222591362126247No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTCGTTGATACCACAGCTTCCCA10.33222591362126247No Hit
GAGTACTTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCCCATGTA10.33222591362126247Clontech Universal Primer Mix Long (95% over 21bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA10.33222591362126247Clontech Universal Primer Mix Long (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAACTGTCTCTT10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCTACACTTTCCCTACACGACGCTCTTCCGATCTATCCCTTTTTTGGGC10.33222591362126247Illumina Single End Sequencing Primer (96% over 31bp)
GTAGGAAAGAGTGTAGATCTCGGTGGTCGCCGATCACCCATGTACTCTGC10.33222591362126247Illumina Single End PCR Primer 1 (96% over 30bp)
AGGTGTGCCGGTCGCGCCATCGCGCGCCATACAGGCAGATCTTCTTGCGC10.33222591362126247No Hit
GTACATGGGATGGGGAACGAGATTACATCTTTCCCTACACGACGCTCTTC10.33222591362126247Illumina Single End PCR Primer 1 (100% over 23bp)
CGCCAAGCGGTGCATCGTCAGCCATTGGTGGCGCGATTCAAGGGCTTCAG10.33222591362126247No Hit
GTACATGGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG10.33222591362126247No Hit
GTACATGGGACGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.33222591362126247Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACATGGGATGTAGCACCGAGATCTACACTCTTTCCCTACACGACGCTC10.33222591362126247Illumina Single End PCR Primer 1 (100% over 35bp)
GGGAAAGAGTGTAGACTCGGTGGTCGCCGTACTTCCCATGTACTCTGCGT10.33222591362126247No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAATG10.33222591362126247No Hit
GTGTCCCGCTCCTCGGTCATCCACACCAGCTTCTTGACCGGGTTGTGCAG10.33222591362126247No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAACATGCGTTTATAC10.33222591362126247No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACCTGTCTCTTATA10.33222591362126247Clontech SMART CDS Primer II A (95% over 21bp)
ACACTATCCTCTTCGTCGGCAGCGTCAGATGTGTATAAGCTGTCTCTTAT10.33222591362126247No Hit
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT10.33222591362126247Clontech SMART CDS Primer II A (96% over 26bp)
ACCTCTTTCTACACGACCTCTCCGATCTCATGATGCAGCACTCCAGTCCT10.33222591362126247No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
AGGAAAGAGTGTAGATCTCGGTGGTGCCGTCACCCATGTACCTGTCTCTT10.33222591362126247Illumina Single End PCR Primer 1 (96% over 25bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.33222591362126247Clontech SMART CDS Primer II A (100% over 22bp)
GATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGGTACCTGCA10.33222591362126247Illumina Single End PCR Primer 1 (100% over 38bp)
GGGTTACAGCCATGTCCGCACGCGCACAGTGAAGCGTCGCCGCGTGCACA10.33222591362126247No Hit
GTGCGTATCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC10.33222591362126247Clontech SMART CDS Primer II A (96% over 26bp)
GAGTGTAGATGTGTGCTATTCCATGTACTCTGCGTTGATACCATTGCTTC10.33222591362126247Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGAGTACATGGGAAG10.33222591362126247Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTATCACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
TGGTATCAACGCAGAGTACATGGGAAGCCTGTCTCTTATACACATCTCCG10.33222591362126247No Hit
GCAGTGGTATCAACGCAGAGTACATGGGACGCAGTGGTATCAACGCAGAG10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGAGATCTCC10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGACGGCCTGT10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATAC10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
ACCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGCGGCCCGAGATCTACACTCTTTCCCTACACGACG10.33222591362126247Illumina Single End PCR Primer 1 (100% over 30bp)
GTACTTTTTTTTTTTTTTTTTTTTTTGGTTGATAACACAGGGTACCATTT10.33222591362126247No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGGTCGACCACCGAGATCTACACT10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GAATGATACGGCGACCACCGAGTCTACACTCTTTCCCTACACGACGCTTT10.33222591362126247Illumina Single End PCR Primer 1 (96% over 28bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGAACATG10.33222591362126247Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCTG10.33222591362126247Clontech SMART CDS Primer II A (96% over 26bp)
CTCTGGACCGACAGAATCTGAGGCAAGAGATCGGAAGACGCGTGTAGAAG10.33222591362126247No Hit
ATCTACCTCTGTCCCAAGTTCTGCAGGTACCAGAGATCGGAAGAGCGTCG10.33222591362126247No Hit
GTAGATCTCGGTGGTCGCGTATCACCCATGTACTCTGCGTTGATACCACT10.33222591362126247Clontech SMART CDS Primer II A (95% over 22bp)
ATCCAGGCTCAGGTCGTGCGCGCGCTGCACGAGGCTGCCGGGCGCGGCGA10.33222591362126247No Hit
ATGGGTGAGGACACCGAGATCTACACTCTTTCCCCAGCAACTCCAGTCCT10.33222591362126247Illumina Single End PCR Primer 1 (95% over 24bp)
CTATAAACGAAAAAAACATAGGAAAAACCACACACAAATACACATCAAAC10.33222591362126247No Hit
GTCACAACGCTTTGCTTTTTGCGTTGCTCTTTTTGTTTCTTCTGGTACTC10.33222591362126247No Hit
CCCATGTACTCTGCGTTGATACCACTGCTCCCATGTACTCTGCGTTCTGT10.33222591362126247Clontech SMART CDS Primer II A (96% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGT10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC10.33222591362126247Clontech Universal Primer Mix Long (96% over 25bp)
CAGAGTACTTTTTTTTTTTTTTTTTTTTTTGGTTGTTACCACAGCTTCCC10.33222591362126247No Hit
TCAACGCAGAGTACATGGGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAA10.33222591362126247Clontech Universal Primer Mix Long (95% over 22bp)
GTCAACGGTAAGCCGGTCATGGAGGTGCGGGCCATGACCGCCGATGGCAA10.33222591362126247No Hit
CATAAATAAACTAATTCACTACACTAACAAATAAACAAATCAACAAGCAA10.33222591362126247No Hit
CACCAGAGATCTACACCTTTCCCTACACGACGCTCTTCCGATCTATCCCT10.33222591362126247Illumina Single End Sequencing Primer (96% over 29bp)
GTACATGGGATAGAACGAGATCTACACTCTTTCCCTACACGACGCTCTTC10.33222591362126247Illumina Single End PCR Primer 1 (100% over 35bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTTAT10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCCAAAAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACTGTC10.33222591362126247No Hit
CTTCCGATCTATCCCTTTTTTGGTACCAACAGGCCAAAAAAGGGATAGAT10.33222591362126247No Hit
CACTTTATAAAAAACCAAACAAAGAACACACCAGACTCAAACAACACTAA10.33222591362126247No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
TATCAAAGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGAACAGAT10.33222591362126247Clontech Universal Primer Mix Long (96% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTATGCGTTTATACCACAGCTTCCC10.33222591362126247No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT10.33222591362126247Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGCGGGACACC10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGTACGCGAC10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAAAAAAAAAAAAAAAAAAAAAAAGTAATCTGCGTTGAAACCCCTGC10.33222591362126247No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTT10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
CGGTGACGATGCAGAACTGCGAGACGTCGGCGCCCGTGCCCGAGGTGCTC10.33222591362126247No Hit
ATCTACACTCTTTCCCTACACGACGCTCTTGCCTCAGATTCTGTCGGTAC10.33222591362126247Illumina Single End PCR Primer 1 (96% over 32bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA10.33222591362126247Clontech SMART CDS Primer II A (96% over 26bp)
ATCAACGCAGAGTACATGGGATGATCGCGACCACCGAGATCTACACTCTT10.33222591362126247Illumina Single End PCR Primer 1 (100% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACGGTAACAGCCCGATCTCGTA10.33222591362126247No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAGAGGCGTTTTAACC10.33222591362126247No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCCTGTCT10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
GTGCAAGCTTAGAACAAATCACAGAATACACCGAAAAAGCAGGTCTTCCT10.33222591362126247No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTAACAGCCCGATCTCGTAT10.33222591362126247No Hit
GATCTCTAAACAGGATGAGTCCGGTATGCCCGTCGTGTAGGGAAAGAGAT10.33222591362126247No Hit
CCTGTGTACAGTGCACCGGGGAGTAATCCCAGCCACGGCAGGCTACCGGC10.33222591362126247No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTAACTGTCTCTTATACACA10.33222591362126247No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.33222591362126247Clontech Universal Primer Mix Long (95% over 21bp)
CTGTCCCAGGTCTTGTCGATACCAGAACAGATCGGAAGAGCGTCGTGTAG10.33222591362126247Illumina Single End PCR Primer 1 (100% over 22bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10.33222591362126247Clontech SMART CDS Primer II A (100% over 24bp)
GGTATCAACGCAGAATACATGGGACACTCTTTCCCTACACTGTCTCTTAT10.33222591362126247No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCCAGACGCGCTTCGCGCGTGGGACACGCTTAGCGTGTCCCTGCTGGAG10.33222591362126247No Hit
GAGTGTAGATCTCGCCCATGTACTCTGCGTTGATACCACTGCTTCCATGT10.33222591362126247Clontech SMART CDS Primer II A (96% over 26bp)
CCTACAGACTGATTACCTCGATCTTTATCAGGTGCACTGGCCGCAGCGCC10.33222591362126247No Hit
GAGCAGGGCTCGATGCGCTGCGACGTCAACCTCTCGCTCCGACCGAAGGC10.33222591362126247No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGATGACGCCGAT10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAGTCTGCGT10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
CATCGCAACCTCCTTCGGATCCCGACCATAACGCGCGGGGACCGGGCCGG10.33222591362126247No Hit
GTATCAACGCAGAGTACATTGGAAGAAGTGGTAAACACGCATAGTAAAAG10.33222591362126247No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACTCTGGGTTGATA10.33222591362126247No Hit
GCGGCTCAAGGGTTTGTCGGTGAAGGCGACGCTGCGGCTTGCCTTTGCCG10.33222591362126247No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATACACATCTC10.33222591362126247No Hit
GGTTTACAGAGTCTACCTCTTCCCTACACGACGCTCTTACGATCTCAGAC10.33222591362126247Illumina Single End PCR Primer 1 (96% over 26bp)
GTTATCAACGCAGAGTACATGGGATGATCGCGACCACCGAGATCTACACT10.33222591362126247Illumina Single End PCR Primer 1 (100% over 21bp)
CTCTTTCCCTACACGACGCTCTTCCGATCTCTGTCCAAGTTCTGCAGGTA10.33222591362126247Illumina Single End PCR Primer 1 (100% over 30bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGTTTAAACACA10.33222591362126247No Hit
ATACATCCCTACCGAGCTCTTCCGATCTCAGTCCCCGCATCATGGTCTGA10.33222591362126247No Hit
ACGCTCTTCCGATCTTGGTACCTGTCCCAAGTTCTGCAGGTACCAAGATC10.33222591362126247No Hit
CTAGATGGCACAACCAACTTCGTGAAAGGTTTGCCACATTTCTCTGTTTC10.33222591362126247No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACAT10.33222591362126247No Hit
GTACATGGGATACCACCGAATTACACTCTTTCCCTGCTGGTACCAGTAGA10.33222591362126247No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTACTATGCATTTATACCAC10.33222591362126247No Hit
GTCTACCCTTCCCTACCGACGCTTGCCTCAGATTCTGTCGGTACCAGAGA10.33222591362126247No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGAAGCAGTGGT10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTCTCTCTACAC10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATAACATAATTAGAAAACAACATCAAAAAAAATTCAAAAAACGAAACAAT10.33222591362126247No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCTGTCTCT10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGATGATACGGCGACCACCGAGATCTACACGGGTTGCAAAA10.33222591362126247Illumina RNA PCR Primer (96% over 30bp)
ATCTACACAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGT10.33222591362126247No Hit
CCACCCAGCGCGAGAGGGCGAGGGCGAGCCTCGCGGGCACGCACGCCCAC10.33222591362126247No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA10.33222591362126247Clontech Universal Primer Mix Long (95% over 22bp)
GAAGCAGTGGTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAA10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGACCCATGTACTCTGCGTTGATACCACTGCTTCCCATG10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGGGGGGGGGG10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCTGGAAGATGCGTTGCGAGTGCACGGCAAGCCGGAGGTGTTCAACAGC10.33222591362126247No Hit
CGGTACACGTGCTCCGCAGCCGCATACCGTCAAAGCCCCGACCAGGCATG10.33222591362126247No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTGCATGGGCTGT10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCGATCTCTGGTACCGACAGAATCTGAGGCAAGAGCGTCGTGTAGGG10.33222591362126247No Hit
GTTTAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGAGATCTCGTTCC10.33222591362126247Illumina Single End PCR Primer 1 (96% over 32bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTGGGTTGAAACCCCCCCAAGTGA10.33222591362126247No Hit
GGTCTAGCGCGCAGCCCGCGCAGCTGAAGGTGCCCGCGCGGTGCTCGTCG10.33222591362126247No Hit
CCCATGTACTCTGGGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT10.33222591362126247Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAAGTACTTTTTTTTTTTTTTTTTT10.33222591362126247Clontech Universal Primer Mix Long (95% over 24bp)
ACGCAGAGTACATGGGAGATACGCACCGGATACCTCTACACGACGCTCTT10.33222591362126247No Hit
CAACTAACAGAACAACTTTGAACCTCACACATATAAAAAACTCATACCCA10.33222591362126247No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCC10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTGTAGATCTCGGGGTCGGCCCCATGTACTCTGCGTTGATACCCTGTC10.33222591362126247No Hit
TGATCGTACTTCCCACGCAGATAATAAAGAGGTGTCATTAATGTGCTCTT10.33222591362126247No Hit
ATCTACACTCTTTCCCTACACGACGCTCGTTTTTCAGGACCTGTTGGTAC10.33222591362126247Illumina Single End PCR Primer 1 (96% over 30bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGTGCTGTCTCTTA10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGATAACGCGACACGAGATCTACACTCTT10.33222591362126247No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACTATGGGTTTATACCACTG10.33222591362126247No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTG10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCTGGAAGTGCTCCCAAGAATCAGAGAAAGTCATGACACTGTCTCTTAT10.33222591362126247No Hit
TATCAACGCAGAGTACATGGGATACCCCCCGAGATCTGCGTCGTGTAGAT10.33222591362126247No Hit
GAAAGAGTGTAGATCTCGTGGTCGCCTATCATTCCCATGTACTCTGCGTT10.33222591362126247No Hit
GTATCAACGCAGAGTACATGGGGAAGCAGTGGTATCAACGCAGAGTACAT10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCGAGATCTACACTCTTTCCCTACACGACGCTCTTGCCTCAGATTCTGT10.33222591362126247Illumina Single End PCR Primer 1 (97% over 38bp)
GACGTTGACCATGCCTGCCACGCAGGCCAGCGCGACGGCACCGATCCAGA10.33222591362126247No Hit
GCGCTCAGCGGCTCGCCGAGTCGAGCGGTGAATTCCTCGCCGGCGTTGTT10.33222591362126247No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC10.33222591362126247Clontech SMART CDS Primer II A (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATACACAT10.33222591362126247No Hit
TCCCTACACGACGCTCTTCCGATCTCCCTGCTGGTACCAGTAGACAGTGA10.33222591362126247Illumina Single End PCR Primer 1 (100% over 25bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTTTAA10.33222591362126247No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCCCA10.33222591362126247Clontech Universal Primer Mix Long (95% over 21bp)
GTCGGGACTGGCGCAGGTGCATCGAGCGATGAGCGCGGCGGGCTTACCCA10.33222591362126247No Hit
GTGTAGGGAAGGAGTGTAGATCTCGGTGGTCGCCGTACACCCGTGTACTC10.33222591362126247Illumina Single End PCR Primer 1 (97% over 37bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAA10.33222591362126247Clontech Universal Primer Mix Long (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAAACGCAGAGTACTTTTTTTTTTTTTTT10.33222591362126247No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
GATCTACACTCTTCCCTACACGACGCTGATCGGAAGAGCGTCGTGTAGGG10.33222591362126247Illumina Single End PCR Primer 1 (95% over 24bp)
GAAGAGCGTCGTGTAGGGAAAGTGTAACTCGGTCATCCCATGTACCTGTC10.33222591362126247Illumina Single End PCR Primer 1 (96% over 25bp)
CAGTAAAATGCAGAACTTGGGACAGAGTGTAGATCTCGCTGTACCACCAA10.33222591362126247No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTAGAAAAAAAAATA10.33222591362126247No Hit
GCTGTTGGCCGACGTCTCGGCCACCAGTGAAGCCCCCCTGGCCACCAGCG10.33222591362126247No Hit
ACGCAGAGTACATGGGTACACTCTTTCCCTACACAGCAACCTGTCTCTTA10.33222591362126247No Hit
GGGAAAGAGTGTAGATCTCGGGCCGTTCCCCATGTACTCTGCGTTGATAC10.33222591362126247Illumina Single End PCR Primer 1 (95% over 21bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCCTGTCTCTTATACA10.33222591362126247No Hit
GGTATCAACGCAGAGTACATGGGTACGCGCCACCGAGATCTACACTCTTT10.33222591362126247Illumina Single End PCR Primer 1 (100% over 21bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGCTGTCTCTTATACA10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCACCCATGTACCTGTCT10.33222591362126247Illumina Single End PCR Primer 1 (97% over 35bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG10.33222591362126247No Hit
AAATATGCAAGGACAGAATTACATCTCACTAAAACAAAAACAAAACAACT10.33222591362126247No Hit
AACCTACTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTC10.33222591362126247No Hit
GTACATGGGTCGGCGACCCGATCTACACTCTTTCCCAGCAACTCCAGTCT10.33222591362126247No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTGCGTTTAAACCACATCTTCCCA10.33222591362126247No Hit
GGGTGGAGACCAGCAACGCGGGCGTGAACGCGCCGATGAACGACGTCAAC10.33222591362126247No Hit
CATGAATAGGCGCACCGGATCTACACTCTTTCCCTACACGACGCTCTTGC10.33222591362126247Illumina Single End PCR Primer 1 (96% over 33bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATCTGTCTC10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
CCTGAGAACCGCCCCTTGGACTCCGCGTCCCATGGTGTCTCTCCTTATTG10.33222591362126247No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCAAAAAAAAAAAAAAA10.33222591362126247Clontech Universal Primer Mix Long (95% over 21bp)
GCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATCCCACTGCTGGC10.33222591362126247Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTAT10.33222591362126247Clontech Universal Primer Mix Long (95% over 21bp)
GTGTAGCCCTGCGCCTCCCAAGCCTCCCGCGCCACGCCCAGCATCGCCGA10.33222591362126247No Hit
CATTTTTAAAAAAAAAGACAACAACGTTACTCTAACTAAAAAACAAAAAA10.33222591362126247No Hit
TTCCTCCGCAACATGATTACCGGTGCGTCGCAGGCCGACGCCGCGGTGCT10.33222591362126247No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.33222591362126247No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCAACGTTGATACCTGTCT10.33222591362126247Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCACGAGATCTACCTCTTTCCCTACACGACGCTCTTCCGATCTCTGGTAC10.33222591362126247Illumina Single End Sequencing Primer (96% over 31bp)
CCCGAGATCTACACTCTTTCCCTACACGACGCGCTGGCGATACCCGTAAA10.33222591362126247Illumina Single End PCR Primer 1 (96% over 32bp)
ACGCTTTCCGATCTCATGATGCAAGGACTGGAGTTGCGTCGTGTAGGGAA10.33222591362126247No Hit
CATGTACTCTGCGTTGATACCACTGCTTGCCATGTACTCTGCGTTGATAC10.33222591362126247Clontech Universal Primer Mix Long (96% over 28bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAATG10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCAGTGCTTCCCATGTACTCTGCGTTGAT10.33222591362126247Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACATGGGCAGACGTGTGCTCTTCCGATCTGGCTTTTAACTCTTCTTAA10.33222591362126247Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGATACGGCGACCACGAGATCTACACTCTTTCCCTACACGACGGAGCTG10.33222591362126247Illumina Single End PCR Primer 1 (96% over 30bp)
GGGAAAGAGTGTAGATCTCGATGGTCGCCGTACACCCATGTACTCTGCGT10.33222591362126247Illumina Single End PCR Primer 1 (96% over 32bp)
CACGACGCTCTTCGATCTCATGTTTACTGGTATCGGCAGCTCCGTGTAGG10.33222591362126247No Hit
AGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTATACACATCTCCGAG10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 21bp)
GTACTTTTTTTTTTTTTTTTTTTTTTCGTTGATACCACAGCTTACCCTGT10.33222591362126247No Hit
ACGCAGAGTACATGGGCAGACGTGTGCTCTTCCGATCTAATGGTTAAGCA10.33222591362126247Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)