FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003652778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003652778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA92.990033222591362Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG82.6578073089700998Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA51.6611295681063125Clontech Universal Primer Mix Long (95% over 21bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC51.6611295681063125Clontech SMART CDS Primer II A (96% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.9966777408637874No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG30.9966777408637874Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA30.9966777408637874Clontech Universal Primer Mix Long (95% over 22bp)
CTTCCGATCTCTGGTACCGACAGAATCTGAGGCAAGAGCGTCGTGTAGGG30.9966777408637874No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT30.9966777408637874Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTATCGCCCTCATCGCTGAGAACACCGGCCACGTCAAAGCGGTCGCTGA20.6644518272425249No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA20.6644518272425249Clontech Universal Primer Mix Long (96% over 26bp)
CCATCACTGGTCGTCATTAGAGAAATGCAAATCAAAACCACAATGAGATA20.6644518272425249No Hit
GCTCATGTACTCTGCGTTGATACCACTGCTTCCCATCTGTCTCTTATACA20.6644518272425249Clontech Universal Primer Mix Long (96% over 26bp)
CACCAGATTACACTCTTTCCTACACGACGCTCTTCCGATCTCTGGTACCT20.6644518272425249Illumina Single End PCR Primer 1 (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACACT20.6644518272425249Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA20.6644518272425249No Hit
ACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.6644518272425249No Hit
CGCCGACATTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC20.6644518272425249Clontech SMART CDS Primer II A (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG20.6644518272425249Clontech Universal Primer Mix Long (96% over 26bp)
TGGCTGCAGCACCCGTGCTTGCTATAGGTAATAGAATTGCCTCTGGATAT20.6644518272425249No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA20.6644518272425249Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGATGTACGGCGACCACGAGTCTACACTCTTTCCCT20.6644518272425249No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTAGGACCAGAG20.6644518272425249Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTGACGCTG20.6644518272425249No Hit
GTACATGGGTGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACG20.6644518272425249Illumina Single End PCR Primer 1 (100% over 41bp)
TTACGCACCACCAGACTACCTCTTCCCTACACGACGCTCTTCCGATCTCT20.6644518272425249Illumina Single End Sequencing Primer (96% over 27bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT20.6644518272425249Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.6644518272425249Clontech Universal Primer Mix Long (96% over 26bp)
TCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA20.6644518272425249Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGATGATACGGACCACCGAGATCTACACTC20.6644518272425249No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTT20.6644518272425249Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGCTGT10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
GCAAAAAAAAAAAAAAAAAAAAAAAGTACTCTGCGTTTATACCAATGCTT10.33222591362126247No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAA10.33222591362126247Clontech Universal Primer Mix Long (95% over 21bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
GTCGTAGGGCCTGGCTGGCCGGTCGGGACGGCAGGCTGACGAGACGCAGC10.33222591362126247No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTGGCGTTGATCTGTC10.33222591362126247No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGAGCGATACA10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTA10.33222591362126247Illumina Single End PCR Primer 1 (100% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAATGATGTAATAA10.33222591362126247No Hit
GCGGAGCTTGGCACAGCGCCAGCATGACGTCACTAGATTGACGTCACTTC10.33222591362126247No Hit
GAGTGTAGATCCGGTGGTCGCGTACTTCCCATGAACTCTGCGTTGATACC10.33222591362126247No Hit
AAAAAGTACTCTGCGTTGATACCACTGCCCATGTACTCTGCGTTGATACC10.33222591362126247Clontech SMART CDS Primer II A (95% over 24bp)
CTCCGGTGCCGGTCTCCACGCTCAGATCCTCTTCTGCACGTCCAGGCACC10.33222591362126247No Hit
CCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTGTACT10.33222591362126247Illumina Single End PCR Primer 1 (100% over 45bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTT10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
GGTGAACCGACTAAAGAGCGTATTACGTGCGCCAGCGGGTTTTGCACCAT10.33222591362126247No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTT10.33222591362126247No Hit
CCTCTGGGCACCAGATGTATTCGCCCGCAGCGATATCTATGGGTAAGCCC10.33222591362126247No Hit
GAGCCCGAGCAGGCGATAGGCGCCGCGGCCGTCCGACTCGATCAGGAGGT10.33222591362126247No Hit
CCAGAGAGCGTGTATGCAGAAAATAGCCCAAAAATTAATGACGCCGTATT10.33222591362126247No Hit
CTTGCTGTGTAGGGAAAGAGTGTACCCATGTACTCTTCGTCTGTCTCTTA10.33222591362126247No Hit
GAGTACATGGGTAGGCGACCACCGAGACTACATCTTTCCCTACACGACGC10.33222591362126247No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAATTAATATGCGTTTATACCAA10.33222591362126247No Hit
GTATCAACGCAGAGTACATGGGTGGGCGCCCGAGATTCACTCTTCCCTAC10.33222591362126247No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTACTGTCTCTTATACACAT10.33222591362126247No Hit
CCCTGCCTGCCCCCGTCCCAGCCTTCCCCCAGAGGGGGAAGGAGCAAAAG10.33222591362126247No Hit
TCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTAG10.33222591362126247Illumina Single End PCR Primer 1 (100% over 22bp)
GTACATGGGATGACCGAGTTACACTTTCCCTACACGACGCTCTTCCTGTC10.33222591362126247Illumina Single End Sequencing Primer (96% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTT10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
CCTCTCTCGCAAACGGGCTACGGTAATTGGGTCGGCGCGTCCGGTTGAAG10.33222591362126247No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA10.33222591362126247Clontech Universal Primer Mix Long (96% over 25bp)
CTCCACGACAGTGCGATCGCAACCGCGTCACCTGGCAACGCCCAGGCAGC10.33222591362126247No Hit
GTATCAACGCAGAGTACATGGGAATGATCGGCGACCCCCCAGATCAAAAC10.33222591362126247No Hit
TTACAGGAGAGCAAGGAAAAGTTTCGCAGGAACCTTTAGCAAGAGATTCT10.33222591362126247No Hit
GAGTACATGGGAGCACCGGATACCTTCCTCCTTGCCTCAGATTCTGTCGG10.33222591362126247No Hit
GTACATGGGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTATACA10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTCCCATGTACTCTGCGTTGATA10.33222591362126247Clontech SMART CDS Primer II A (96% over 25bp)
TTCCTACACGACGCTCTTCCGATCTCATGATGCAAGGACAGCAACTCCAG10.33222591362126247Illumina Single End PCR Primer 1 (96% over 25bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAGAAGCAGTGGTA10.33222591362126247No Hit
GAGACGGGGCGCCCGGTCCCGGTCCGCATGTCCCGGAGACACCGTCATGC10.33222591362126247No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAATTAATAGGCGTTTGATAACAAAA10.33222591362126247No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACA10.33222591362126247No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAGTAC10.33222591362126247Clontech Universal Primer Mix Long (95% over 21bp)
GCAGCTCCGTCGTGTAGGGAAAGAGTGTAGATCTCGTGGTCGCCGTATCC10.33222591362126247Illumina Single End PCR Primer 1 (96% over 30bp)
GTGATGATGGCCAGCAACGAGATCTGCATGTTGAACAGGCGCTGCGCATC10.33222591362126247No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCTCTTATAC10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACGACGCTCTTCCGATCTGTCCCAGGTCTTGTCGATACCAGAACAGAT10.33222591362126247No Hit
CCTCTACACGACGCTCTTCCTCAGATTCTGTCGGTACCAGAGATCGGAAG10.33222591362126247No Hit
CAACGCAGAGTACATGGGCGGAACGAGATCTCACTCTTTCCCTACACGAC10.33222591362126247No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGACTACATGGGAAGCAGTGGTA10.33222591362126247Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGGGCCTGTCTCTTA10.33222591362126247Illumina Single End PCR Primer 1 (97% over 39bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTG10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTAT10.33222591362126247Clontech Universal Primer Mix Long (95% over 21bp)
GCACCGCCGCGTGCCGGCAGACCTCGTAGCGGACCGCCCGCTCGACGCTG10.33222591362126247No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGT10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
CCCTACACGACGCTCTTCCGATCTCATGATGCAAGACTGGAGTTGCTGGG10.33222591362126247Illumina Single End PCR Primer 1 (100% over 24bp)
GTCCTGAAAAACGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGGCCGC10.33222591362126247Illumina Single End PCR Primer 1 (96% over 33bp)
GTATCAACGTTGAGTACATGGGAAGCAGTGGTATCAACGCAGAGCTGTCT10.33222591362126247Clontech Universal Primer Mix Long (95% over 22bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.33222591362126247Clontech Universal Primer Mix Long (100% over 23bp)
GCCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAATGATACGGCGACACCGAGACACGACGC10.33222591362126247No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTAAAAAAATAATAC10.33222591362126247No Hit
GATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTT10.33222591362126247Illumina Single End PCR Primer 1 (100% over 38bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTAA10.33222591362126247No Hit
CTCTTTCCCTACACGACGCATACCGGACTCATCCTGTTTAGAGATCGGAA10.33222591362126247No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
CTCCTACGGATATTCGGATGCCCTCAAGGACGGCGTGGTTCGCCCCGTCG10.33222591362126247No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAA10.33222591362126247Clontech Universal Primer Mix Long (95% over 22bp)
GGATATAGACCAGCTACACTTCCCCTGCTGGTACCAGTAGACAGTGAGAT10.33222591362126247No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA10.33222591362126247Clontech Universal Primer Mix Long (95% over 22bp)
GGGCGGCGCCTGCCAGATGCCGCCTCGGATGTCGCCCCAGGTGTCGTCCG10.33222591362126247No Hit
TATCACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
GTCTGGATCGGTGCCGTCGCGCTGGCCTGCGTGGCAGGCATGGTCAACGT10.33222591362126247No Hit
GGTATCAACGCAGAGTACATGGCATGATACGGCGACCACCGAGATCCTGT10.33222591362126247Illumina Single End PCR Primer 1 (95% over 24bp)
ACACGGAGCTGCCGATACCAGTAAACATGAGATCGGAAGAGCGTCGTGTA10.33222591362126247Illumina Single End PCR Primer 1 (100% over 21bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCCATTTAAACGGCGTT10.33222591362126247No Hit
TTCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTCTGCAGGTACCA10.33222591362126247Illumina Single End PCR Primer 1 (100% over 26bp)
GATAAAGACGTCCGCAGCCGCGCGCAGTCTTGACCGCACTTCTTGCACGT10.33222591362126247No Hit
CCGCCGCTCGGACCCGCGGTCTCGTGCTCGGTGAGGAGCAGTCTGACGCC10.33222591362126247No Hit
GTACATGGGAAGCAGTGGTATCAACCCAGAGTACATGGGCTGTCTCTTAT10.33222591362126247Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CATCTTCTACACGCGTCTTCCGATCTCTTGCCTCAGATTCTGTCGGTCCA10.33222591362126247No Hit
CTTCCGATCTCTGGTACCGACAGAATCTGAGGCAAGCGTCGGTAGGGAAG10.33222591362126247No Hit
TTCCCTACACGACGCTCTTCCGATCTGTTCTGGTATCGACAAGACCTGGG10.33222591362126247Illumina Single End PCR Primer 1 (100% over 26bp)
AACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGAGTACATGGGCTGT10.33222591362126247Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCCTGAC10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
CAGCTGGATCGCGCCATGGGTCAGGGGCTTGCCATCGGCGGTCATGGCCC10.33222591362126247No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT10.33222591362126247Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTC10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGATGATACGGCG10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCCGATCTCTGGTACCGACGAATCTGAGGCAGCGTCGTGTAGGGAAAG10.33222591362126247No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTT10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGGGGTATCAACGCAGAGTACTT10.33222591362126247Clontech SMART CDS Primer II A (96% over 26bp)
GCGTTGGCATGCGCGCATGCCTGGTCGGGGCTTTGACGGTATGCGGCTGC10.33222591362126247No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAATGCGTTTAAAACACA10.33222591362126247No Hit
ACGCAGAGTACATGGGAAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAA10.33222591362126247Clontech Universal Primer Mix Long (95% over 23bp)
ACGCTCTTCCGATCTCTGGTACCTGTCCCAAGTTCTGCAGGTACCAGAGA10.33222591362126247No Hit
ATCTAATGGTTAACACTGCTTAACCATTAGATCGGAAGAGCACACGTCTG10.33222591362126247Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCAACGCAGAGTACATGGGTGCGGGACACGAGACTACACTCTTTCCCTAC10.33222591362126247No Hit
GTACATGGGATGTACGGCGACTACCGAGATCTACACTCTTTCCCTACACG10.33222591362126247Illumina Single End PCR Primer 1 (100% over 28bp)
CTTCCGATCTATCCCTTTTTTGGGCCTGTTGGTACCAAAAAAGGGATAGA10.33222591362126247No Hit
GTTCAAATTTGAGTACCAGAAGAAACAAAAAGAGCAACGCAAAAAGCAAA10.33222591362126247No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCCAAAAAAAAAA10.33222591362126247No Hit
GTACATGGGAAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA10.33222591362126247Clontech SMARTer II A Oligonucleotide (95% over 23bp)
GTACATGGGTCGGGGGGGGCCACGGAGAAACAAAACCCCACCGACGCCTA10.33222591362126247No Hit
CGGAAGAGCGTCGTGTAGGGAAAGGAGATCTCCCCATGTACTCTGCGTTG10.33222591362126247Illumina Single End PCR Primer 1 (95% over 24bp)
GAGTGTAGATCTCGGTGGTCGACCCCATGTACTCTGCGTTGATACCACTG10.33222591362126247Illumina Single End PCR Primer 1 (95% over 23bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTGGCGTTGATACCAATGCTTCCCA10.33222591362126247No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTAATATGCGTTTATACCAATG10.33222591362126247No Hit
TATCAACGCAGAGTACATGGGTTCGGGCCACCGATCTACACTTTTCCCTA10.33222591362126247No Hit
TCGGAAGAGCGTCGTGTAGGGAAAGATGTAATCTCGTTCCCCATCCCATG10.33222591362126247Illumina Single End PCR Primer 1 (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCCTGTCTCTTATA10.33222591362126247No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG10.33222591362126247No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCT10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTATACCAATGGT10.33222591362126247No Hit
CCCATGTACTCTGCGTTGATACCACTCTGTCTCTTATACACATCTGACGC10.33222591362126247Clontech SMART CDS Primer II A (95% over 22bp)
ATACGCGACCCCGAGATCTACCTCTTTCCCTACACGACGCTCTTCCGATC10.33222591362126247Illumina Single End PCR Primer 1 (96% over 30bp)
GTGTAGGGAATTAACTCGGGTCACCCATGTACTCTGCGTTGATACCACTG10.33222591362126247Clontech SMART CDS Primer II A (95% over 23bp)
GTGGTGTACACGGTGAGCAAGTACAGCCCGCTGTCGCTGGTCGCCTGGGC10.33222591362126247No Hit
GACTATGACAACTATCTGTCAAACCACAATATCTCCCCGCGCTTTATGAC10.33222591362126247No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGT10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
TTACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACA10.33222591362126247No Hit
GTACATGGGCGATCAATCTTCCCTACACGACGCTTGCCTCAGATTCTGTC10.33222591362126247No Hit
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA10.33222591362126247Clontech Universal Primer Mix Long (95% over 21bp)
TTCTATTGCTATCCGTGTAAAAAACCGTACCCACTTCGGTACGGTTTTTT10.33222591362126247No Hit
GCCTTCTCGGATGCGCGAACCGACGTGAAATCTTCGTAAGATGACTAGCA10.33222591362126247No Hit
ACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA10.33222591362126247Clontech Universal Primer Mix Long (95% over 22bp)
AACCAGTTCGCGGTGCTGCGCCAGGAAGGCACCGAGCGGCCCTACACCAG10.33222591362126247No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAATTAATATGCGTTTAAACCACTG10.33222591362126247No Hit
TGTCATGACTTTCTCTGATTCTTGGGAGCACTTCCAGCACTGTCTCTTAT10.33222591362126247No Hit
TCTACACTCTTTCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGA10.33222591362126247No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCTCTTAT10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
CTTTCCTACACGACGCTCTTCCGATCTCATGATGCAAGGACTGGAGTTGC10.33222591362126247Illumina Single End PCR Primer 1 (100% over 23bp)
CCCTACACGACGCTCTTCCGATCTCACTGTCTACTGGTACCAGCAGGGAA10.33222591362126247Illumina Single End PCR Primer 1 (100% over 24bp)
CTTCCGATCTATCCCTTTTTTGGCCTGTTGGTTCCAAAAAAAGGGATAGA10.33222591362126247No Hit
GTACTCTGCGTTGATACCGCTGCTTCCCATGTACTCTGCGTTGATACCAC10.33222591362126247Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGTAGGGAAAGAGTGTCCCATGTATTCTGCGTTGATACCCTGTCTCTTAT10.33222591362126247No Hit
AATCTGAGGCAAGAGCGTCGTGTAGGGAAGAGTGTAGACTCGTTGCCGTT10.33222591362126247No Hit
ATCATATGCAATAAGGAGAGACACCATGGGACGCGGAGTCCAAGGGGCGG10.33222591362126247No Hit
CATGGGGTCTCTCTACACGACGCTCTTCCGATCTCTGGTACCTGCAGAAC10.33222591362126247Illumina Single End Sequencing Primer (96% over 25bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
AGCATATTCAACGCTTTGCACTGGATGATGCCCAACATAGAGCTCCGTTC10.33222591362126247No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAATTAC10.33222591362126247No Hit
ACACTGCGCCACAGCCTTTCAACGAAGATGTTGTCCAGCGCCCGCCCGCG10.33222591362126247No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAGTAATCTG10.33222591362126247Clontech Universal Primer Mix Long (95% over 22bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGTGATACGGCGACCACCGAGATCTACACTCTTTCCCCTGTCT10.33222591362126247Illumina Single End PCR Primer 1 (97% over 35bp)
GGACGATGCGGAAGCCGAAACCGAAGCGAAGCCTGCGGCAGCCGCGAGGA10.33222591362126247No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAATGG10.33222591362126247No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTG10.33222591362126247No Hit
GAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG10.33222591362126247Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA10.33222591362126247Clontech Universal Primer Mix Long (96% over 25bp)
CCCCGATCCCGCCGGTGCTCATCATCCCGAGCACCTCGGGCACGGGCGCC10.33222591362126247No Hit
GTATCAACGCAGAGTACATGGGGAAGCAGTGGTATCAACGCAGAGTACAT10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGAAGAGCGTCGTGTAGGAAAAGTCTCGGTGTCCCGTCCCATGTACTCTG10.33222591362126247Illumina Single End PCR Primer 1 (95% over 23bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGTACGGCGA10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTTGCCTCAGATTCTATCGGTACCAGAGATCGGAAGAGCGTCGTGTAG10.33222591362126247Illumina Single End PCR Primer 1 (100% over 22bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACACAT10.33222591362126247No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAATTAATCTGGCGTTTATACCAC10.33222591362126247No Hit
GTACATGGGTGACGGCACCACCGAGATCTACACTCTTTCCTCTGTCTCTT10.33222591362126247Illumina Single End PCR Primer 1 (95% over 24bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTCTGTCTC10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAAGTGGTATCAACGCAGAGTACATG10.33222591362126247Clontech SMARTer II A Oligonucleotide (95% over 22bp)
TATCAACGCAGAGTACATGGGAATGATACGGCGACCACCGAGATCTACAC10.33222591362126247Illumina Single End PCR Primer 1 (100% over 29bp)
GTACTGGGGATCGGCGACCCCCCAGATAAAAACACATTACCAAACCCCCG10.33222591362126247No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
CACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTA10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAATTAAGCGTT10.33222591362126247No Hit
CTCTTCCGATCTGGTACCAACAGGTCCTGAAAAACGAGCGTCGTGTAGGG10.33222591362126247No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGTCTCTTATACACATCTGAC10.33222591362126247Clontech SMART CDS Primer II A (95% over 23bp)
TCCCTACACGACGCTCTTCCGATCTATCCCTTTTTTGGTACCAACAGGCC10.33222591362126247Illumina Single End PCR Primer 1 (100% over 25bp)
GTCGGTTTCTTAGTCCTGCGGACATCGCATCGGTCACCAAATAGCCTTGC10.33222591362126247No Hit
CATGTTAAGCAGTGGTATCAACGCAGAGTACGTGGGAAAGGCCACAGATT10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCGCAGCTGTCTCTTAT10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAAAAAAAAAAAAAGAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCA10.33222591362126247No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCCAGAGTACTTT10.33222591362126247Clontech SMART CDS Primer II A (96% over 26bp)
GGCAAGAGCGTCGTGTAGGGAAAGATGTAGATCTGGTGTCCCGCTCCCAT10.33222591362126247Illumina Single End PCR Primer 1 (95% over 22bp)
AGAGCTGGCATACCAGGCGATCGACGGCGTACGCTCGGCGACACCATCGC10.33222591362126247No Hit
AAAAAAAAAAAAAAAAAAGTACACTGGCGTTGATACCACTGCTTCCCCAT10.33222591362126247No Hit
CCCATGCACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGT10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGTACGGCGACCCCGAGATCTACACTC10.33222591362126247No Hit
GAACATGCCTGAGCGGCGATGCGTGAGTGCCATGAGCCCGGCCGCTGCGT10.33222591362126247No Hit
ACGTTAAGAAGAGTTAAAAGCCAGATCGGAAGAGCACACGTCTGCCCATG10.33222591362126247Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTAGGGAAA10.33222591362126247Illumina Single End PCR Primer 1 (100% over 27bp)
AGAGATCGGAAGACGTCGTGTAGGGAAGAGTGTAGATGGCCTACTCCCAT10.33222591362126247No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTGTTTATAT10.33222591362126247No Hit
GGGAAGCAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAGTAC10.33222591362126247Clontech Universal Primer Mix Long (95% over 24bp)
AGACTACACCTTCCCTACACGACGCTCTTGCCTCAGATTCTGTCGGTACC10.33222591362126247Illumina Single End PCR Primer 1 (95% over 21bp)
GTCCCAAGTTCTGCAGGTACCAGAGATCGGAAGAGCGTCGTGTAGGGAAA10.33222591362126247Illumina Single End PCR Primer 1 (100% over 27bp)
GTCGTGATGGGACCAAGATGCTGAGCTTGCCTTGCTTGCACCGATCCTGT10.33222591362126247No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTAAAAC10.33222591362126247No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCACTGGTATCAAC10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACAGGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT10.33222591362126247Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAATGGTATCAACGCAG10.33222591362126247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.33222591362126247Clontech Universal Primer Mix Long (95% over 21bp)
GGTCACGGTCTCTGCAAACGTTAAACCGCGACCGGCGTTCGTCATCGTGA10.33222591362126247No Hit
CAGATTACATGGGTGATACGGCGACACCGAGATCTACCTCTTTCCCTACA10.33222591362126247No Hit
GGATGACGCCGATCTACCTCTTTCCTACACGACGCTCTTCCGATCTCTGT10.33222591362126247Illumina Single End PCR Primer 1 (100% over 23bp)
TCGCGCTCCTTAGCCGTGGTCGCGGTGAGCAACATCTTCTGTGCGCGGCT10.33222591362126247No Hit
GGTATCAACGCAGAGTACATGGGGCCGACCCCGAGATCTACACTCCTGTC10.33222591362126247No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTCTGTCTCTTAT10.33222591362126247No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTGGGCGTTTATAACACAGCGTC10.33222591362126247No Hit
CAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAATTATTTA10.33222591362126247No Hit
AACGCAGAGTACATGGGAAGCAGTTGTATCAACGCAGAGTACATGGGAAG10.33222591362126247Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCT10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
TACACGACGCTCTTCCGACTCATGTGCAAGGACTGGAGTTGCTGGGGAAA10.33222591362126247No Hit
CTTTCCCTACACGACGCTCTTGCCTCAGATTCTGTCGGTACCAGAGATCG10.33222591362126247Illumina Single End PCR Primer 1 (95% over 23bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACACGG10.33222591362126247Clontech Universal Primer Mix Long (96% over 26bp)
CCCCAACTGTAGCAGGCTGAGTAAATCTCTCACCAGTACCACCATCATAT10.33222591362126247No Hit
TCCCTACACGACGCTCTTCCGATCTAAACCATTTTTTGGGCCTGTTGGTA10.33222591362126247Illumina Single End PCR Primer 1 (100% over 25bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTCCCATGTA10.33222591362126247Clontech SMART CDS Primer II A (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)