FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003652785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003652785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1280
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA443.4375000000000004Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT443.4375000000000004Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG262.03125Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG151.171875Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC131.015625Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG120.9375Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC110.8593750000000001Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT100.78125Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC90.703125Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT90.703125Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA90.703125Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC80.625Clontech SMART CDS Primer II A (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG70.546875Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC70.546875Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT60.46875Clontech Universal Primer Mix Long (100% over 23bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC60.46875Clontech Universal Primer Mix Long (96% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA60.46875Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC60.46875Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG60.46875Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT60.46875Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT50.390625Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC50.390625Clontech Universal Primer Mix Long (96% over 26bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG40.3125Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA40.3125Clontech Universal Primer Mix Long (95% over 21bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC40.3125Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.3125Clontech Universal Primer Mix Long (95% over 22bp)
CATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCG40.3125No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA40.3125Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC40.3125Clontech Universal Primer Mix Long (96% over 26bp)
CATTGACGTTGCGCGGGCACAGCAGGAAAACCTTGCTGGTCACCCTCTCG40.3125No Hit
TCTCACCTTTAGAAAATTATACAAAAATAAACATTGTGGCCGGATGCGGT40.3125No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA40.3125Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG30.234375Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACAGACGTCCAAGAAAAAAACATTTGGTGAAAGTCAGGATTTACCTTT30.234375No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTATA30.234375Clontech Universal Primer Mix Long (95% over 21bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA30.234375Clontech Universal Primer Mix Long (95% over 21bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTT30.234375Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG30.234375Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CATTATTTGTTCTGTTCTTATATGCATGCTTTCCCCCTGCTCCATGGAAT30.234375No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGATACCACTGCTTCCCATGT30.234375Clontech Universal Primer Mix Long (95% over 24bp)
GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT30.234375Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG30.234375Clontech Universal Primer Mix Long (96% over 26bp)
AGATTAGCCACTGTAGCTCAAAGAAAAAAACAGAAAAGAAACCATTGCTC30.234375No Hit
CCTATGTGCCATGCACTGCATTCATTTTTTTACATAATGTCATTGAGAAC30.234375No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTC30.234375Clontech Universal Primer Mix Long (96% over 26bp)
GATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTT30.234375Illumina Single End PCR Primer 1 (100% over 38bp)
ACACGACGCTCTTCCGATCTATCCCTTTTTTGGTACCAACAGGCCAAAAA30.234375No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG30.234375No Hit
GTCACCGGGATCGCCTTGAAGGTCAGGCGGCGCTTCAGATTCTGGTCTTC30.234375No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTATAC20.15625Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.15625No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA20.15625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTCTGT20.15625Clontech SMART CDS Primer II A (100% over 26bp)
CTTCCGATCTCTGGTACCGACAGAATCTGAGGCAAGAGCGTCGTGTAGGG20.15625No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTACC20.15625Clontech Universal Primer Mix Long (96% over 26bp)
TTTCCAGTAACTCCCTATTTTACTTAATAAGGGATAGCCTATTTTTAAAA20.15625No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT20.15625Clontech Universal Primer Mix Long (96% over 25bp)
TGCGCGCGCCCTGGCCTGGCACGAGCGGCGCAAGCGCGAGCTGCTGTACG20.15625No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT20.15625Clontech Universal Primer Mix Long (96% over 26bp)
CTATTGAGCAATAACTATTGAATAATAACAATGAACAATTATTTCAAGCC20.15625No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTC20.15625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGCCTTCACTTCCTGGGCAACTGAGCAAGACCCTGTATCAAAAAATAA20.15625No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGC20.15625No Hit
ATTGTACAATTACCCACCACTGGATTTGACTCAGAGAGGACCCCCAGAGG20.15625No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC20.15625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC20.15625Clontech Universal Primer Mix Long (96% over 25bp)
GGTGAAGGGAAAACTTTTATATGTAATAACAAAGGGAAGTTTTCCCTCTG20.15625No Hit
GTGTAGGGAAAGAGTGTAGATCTCGGGTCGCTACCCATGTACTCTGCGTT20.15625Illumina Single End PCR Primer 1 (96% over 26bp)
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA20.15625Clontech Universal Primer Mix Long (96% over 25bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20.15625Clontech Universal Primer Mix Long (96% over 26bp)
CTATTGACATTACGGACTGGGTAATTATTTGTTATCCAGGGACCGTCCTG20.15625No Hit
GTGCATAGTCTGGCTGCCTTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG20.15625No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTT20.15625Clontech Universal Primer Mix Long (96% over 26bp)
TTTGCAATGAGTTTCTTCTCCCAGTGTTCCCATTGCAGCTCTGTAATTTA20.15625No Hit
AAGTTGAATACTGCTACCATAAAACTATGACGAACATTTTGATAATCACT20.15625No Hit
GCCCTGCTGCTGGCCCCGGGGATCTGAGTCGTACCCTCTTGTTTTTCTCT20.15625No Hit
TTATTGGTTAACAACGATGAGCCAATCTTGACAATAACGCGCTTAATATC20.15625No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGATTACGGCGAC20.15625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC20.15625Clontech Universal Primer Mix Long (96% over 25bp)
TTTCTGCACCTTGTCCCTGGGAGACACCCCGGTCCTGCCAGAATGTGTCA20.15625No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTT20.15625Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGGAGCTGGCCACGCTGCGTGCGCTGGCCGCACATGAGCGCATCGT20.15625No Hit
CATCGATATCTACCGGAACAAGACCATTTGCAAGAGCTGTCGGTTGTATT20.15625No Hit
GACAGAAGGAGGTTTCATCTCAAAGAAAAAAAAGAAGAAGAAGAAGCCAG20.15625No Hit
GATCTACACTCTTTCCCTACACGACGCTCGTTTTTCAGGACCCTGTCTCT20.15625Illumina Single End PCR Primer 1 (96% over 31bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA20.15625Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.15625Clontech Universal Primer Mix Long (96% over 26bp)
CGTCGAGGTATTGCAACTCCTTGCTGAACAGCAGGTCGTCCACGTAGCTG20.15625No Hit
TATATATATCATCTTATTTCGTTATGGCAACAGGACCTGCCATATATCAG20.15625No Hit
CATTTGTTCTGTGCCAGAAACCAGGGGGGAGCACTTTACATGGACTTTTC20.15625No Hit
CCATTCCATTCCATTCCATTCCATTCCATTCCATTCCATTCCATTCCATT20.15625No Hit
CTCGACCCCTGCACGCCGGTGAGCAAGCGGAACCAGTCGCGCTCCGAAGC20.15625No Hit
TTCAATACCAATTGATGATGGTTATTTTTGATTCCATTTGATGAGGATTA20.15625No Hit
AGAGCCTGAACATCCCTTTTTTGCCAGAGGTGAGGGGACAGCCTCTCTCT20.15625No Hit
TCATTGCACTCTTTGAGGAGTACCGTAGTAAAGGAAATAACTTCCTATAA20.15625No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA20.15625Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGCTTCCCATGTACTCTGCGT20.15625Clontech Universal Primer Mix Long (95% over 22bp)
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT20.15625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGATTTAACTCGTTAGATATTTCTGAAAACCAAACACACCTGGTTATGTA20.15625No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC20.15625No Hit
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.15625Clontech Universal Primer Mix Long (96% over 26bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA20.15625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTAGTGAAATAGAGTGGAATGGAGTGGAGTGGAGTGGAATGGAGTGGA20.15625No Hit
GTGTATAAATGTTCTGTTTTATCTGCAGCCTCACCAGCATCTGTTATTTT20.15625No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGCTGTCTCTTA20.15625Clontech Universal Primer Mix Long (96% over 26bp)
CTTTATTTGTCTTGCTAGCGGTCCATCAATTTGTTCATCTTTTCAAAAAA20.15625No Hit
ATACATAGATGCCTCACTGTGTGGCATGAGGTAATGGAGCACCAAGCAGC20.15625No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)