FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003652786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003652786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1280
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA463.5937499999999996Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT352.734375Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG221.7187500000000002Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT181.40625Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG181.40625Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG161.25Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC141.09375Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT90.703125Clontech Universal Primer Mix Long (100% over 23bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT80.625Clontech SMART CDS Primer II A (100% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC80.625Clontech SMART CDS Primer II A (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC80.625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA70.546875Clontech Universal Primer Mix Long (95% over 21bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG70.546875Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT70.546875Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG70.546875Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT60.46875Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA60.46875Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG60.46875Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA60.46875Clontech Universal Primer Mix Long (96% over 26bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA50.390625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC50.390625Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT50.390625Clontech Universal Primer Mix Long (96% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC50.390625Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT50.390625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA50.390625Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA40.3125Clontech Universal Primer Mix Long (95% over 22bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG40.3125Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGT40.3125No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC40.3125Clontech Universal Primer Mix Long (96% over 26bp)
GTTCAACACCGTCGCCGTTCGGAACTTGAGGAGCCGAGCATCGCTGACAT40.3125No Hit
TGCCTCCGGGGCTCAAGTGATTCTCCTACCTCAGTTTCCCAAGTATCTGG40.3125No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG30.234375Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCCGATCTCTGGTACCGACAGAATCTGAGGCAAGAGCGTCGTGTAGGG30.234375No Hit
GATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC30.234375Clontech SMART CDS Primer II A (96% over 26bp)
CTCAGACCGTTTCTGTGAGATCTGTTCTGAGCCATCTTTGGCCACAGCAG30.234375No Hit
GCAATAGACTCTATACAAGGGACTGTGATATGGGCTGGGATGGAAAACTT30.234375No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTC30.234375Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA30.234375Clontech SMART CDS Primer II A (96% over 26bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA30.234375Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATCTGTCTCTTATA30.234375Clontech Universal Primer Mix Long (95% over 21bp)
TATAAAGACTGACTTTTAAGCTGCAATATAGCCAATATACACTGTTAAGA30.234375No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC30.234375Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTT30.234375Clontech Universal Primer Mix Long (96% over 26bp)
ACAAAGACCTGGATTAGAATTCTGGGCTAGCTGTGTGGCCTTGGAAGATT30.234375No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA30.234375No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA30.234375Clontech Universal Primer Mix Long (96% over 26bp)
GTCCATAGGAGACCTCATGAAGAACTGGCTTGCAACGATGGCCGGATGGG30.234375No Hit
ATTCATAGCACTAAATGCCCACAAGAGAAAGCAGGAAAGATCCAAAATTG20.15625No Hit
ACCATGCCGTGGTCGATGTTGGCGGCGTCGGCTATCTCGTCGGCTGCTCG20.15625No Hit
CCGACTACACGCTGCGGCTCTACGCATCGAAGCAATATCTCTCGGCGCAC20.15625No Hit
ACTGCGCGCGCTTCAGCCGCGGCATGGGCGCGGAGATGTGGCGCTCGCCG20.15625No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTCTTA20.15625Clontech Universal Primer Mix Long (96% over 26bp)
CACACACACACACACACACAAACGCACACAAACAATGAAATACTACTCAG20.15625No Hit
GAATAGAATCGACTAGAGTGGAATGGAAAGGAATGGACTCGAATGAAATG20.15625No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTCTGTCTCTTATAC20.15625Clontech Universal Primer Mix Long (96% over 26bp)
GAATGGAATTATCGAATGCAATCGAATGGAATTATCGAATGCAATCGAAT20.15625No Hit
TCTTTGTAGAGATGAGGTCTCACTGTGTTGTCCATGCTGGTCTCAAACTC20.15625No Hit
TATCAACGCAGAGTACATGGGAAGCATGGTATCAACGCAGAGTACTTTTT20.15625Clontech SMART CDS Primer II A (95% over 21bp)
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACC20.15625No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA20.15625Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT20.15625Clontech SMART CDS Primer II A (100% over 26bp)
GGGTTTCACTATGTTGACTGGGTTGGTTTCAAACTTCTGACCTCAGGTGA20.15625No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG20.15625Clontech SMART CDS Primer II A (96% over 26bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGCTGT20.15625Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA20.15625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTT20.15625Clontech Universal Primer Mix Long (96% over 26bp)
GCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGT20.15625Clontech Universal Primer Mix Long (96% over 26bp)
ACATTTGGCAATCTGGATACATTTTTCAGTGTCACAACTTAAGGGAGTGG20.15625No Hit
ATGGAATTCCGACACGAGTTACCTCTTTCCCTACACGACGCTCTTCCGAT20.15625Illumina Single End Sequencing Primer (96% over 29bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG20.15625Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGCTATCAACGCAGAGTACATGG20.15625Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GATCTAGTATCTTGATATAAACTAGAAAATACTCTTTACAAAAACTTCAT20.15625No Hit
TTTTTGATGTTTTGATTTGGGGCTTTTCTTTGTTTCTTTTCTCCACATTA20.15625No Hit
GGTATCAACGCAGAGTACATGGGTGGTGACTCTAGATAACCTCGGGCCGA20.15625No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTT20.15625Clontech Universal Primer Mix Long (96% over 26bp)
CTGACACATTCTGGCAGGACCGGGGTGTCTCCCAGGGACAAGGTGCAGAA20.15625No Hit
GGTCCTGAAAAACGAGCGTCGTGTAGGGAAAGAGTGTAGATCCTGTCTCT20.15625Illumina Single End PCR Primer 1 (96% over 29bp)
GGCCAATGGCCTGATCCAGGGGGCATGCCATGGCTGAGGGCTGGGCTTGG20.15625No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG20.15625Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGAGTACATGGA20.15625Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGACGC20.15625Clontech Universal Primer Mix Long (96% over 26bp)
GTAGTATACGTTCCTTAAATCACGATGCTGAGCAAATATACTTAAGTTTT20.15625No Hit
ACCTATCCCTTTGTCTATCCATTTGCGATGTCGAATTCAAGGCATACTTC20.15625No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA20.15625Clontech Universal Primer Mix Long (96% over 26bp)
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA20.15625No Hit
AAGCTGTAGAGTCAGAAAGATCTGCTATGAATTGATGGTGTTACCTAGGC20.15625No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA20.15625Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GTATTTTCCTCAGGCAGTTTGGGCACTGTGGGTGAGCCCAGAGGGTGAAT20.15625No Hit
CGGTGTCGCCGTAATCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT20.15625Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTACCATGTAC20.15625Clontech Universal Primer Mix Long (96% over 26bp)
TCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAG20.15625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT20.15625Clontech Universal Primer Mix Long (96% over 26bp)
GATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTGTCCCAGGTCTT20.15625Illumina Single End PCR Primer 1 (100% over 38bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTC20.15625Clontech Universal Primer Mix Long (96% over 26bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA20.15625Clontech Universal Primer Mix Long (96% over 26bp)
GCATATACAATCATTAACAGTTCACAGACTATCTCCCCTTCAACTGATTT20.15625No Hit
GTGTGCATAATGTAAAATCAATAAATAATTAACTGCTACTGATTGTAATT20.15625No Hit
ACTCGGGGATTTGAATGCAAACATCACAAAGAAGATTCTGAGACTGCTTC20.15625No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.15625No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA20.15625Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC20.15625Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG20.15625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTGATTAACTCATGACAGTGAAGTCCTCATGAATGGGATTAGTGCACTC20.15625No Hit
TTGTCACACAGTGCTGAACACATGGTAGTATCTACCATTTGTTGAGCATC20.15625No Hit
GCTGGGATTACAGGCACGAGCCATGGCACCCAGCCTCCTGGGTTTTAAGT20.15625No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG20.15625Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGTTGTTCAATATCATTATTCACCCAACCGGTCTCAGCATCAGATT20.15625No Hit
GGATGAAGTGGACACAGCATGTGAAGACAATTCCTGTCTTTTCAATTCAA20.15625No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT20.15625Clontech SMART CDS Primer II A (100% over 26bp)
ACTCCACTCCATTCCTCTTCATTCCATTCCCTTCCACTCCATTCAATTCC20.15625No Hit
TAGTTTTACAGTGATTATCAAAATGTTCGTCATAGTTTTATGGTAGCAGT20.15625No Hit
CAAGAGTAAATTACAGAGCTGCAATGGGAACACTGGGAGAAGAAACTCAT20.15625No Hit
CACTATGTATCCAATAGAGGTCTAATGTCTAGAATCTATGAGGAACGTAG20.15625No Hit
GATAAAAACTGTATGAAAGCAGGGAGTGGATTTTAAAACCTGAGCTGCTC20.15625No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)