FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003652791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003652791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA165.194805194805195Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG103.2467532467532463Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT72.272727272727273Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT61.948051948051948Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG41.2987012987012987Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC41.2987012987012987Clontech SMART CDS Primer II A (96% over 26bp)
CTCTTATACACATCTCCGAGCCCACGAGACCATCCTTCAGGTATCTCGTA20.6493506493506493No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTACT20.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCGCCGCACCATGAACGACGCCGCAGACATCTCCAACAGCATCCGG20.6493506493506493No Hit
ACGCTCTTCCGATCTATCCCTTTTTTGGGCCTGTTGGTACCAAAAAAGGG20.6493506493506493No Hit
ACCGCACCCGGAGCACGTCGCGCTCGCGCAGCAGCGCGCACGCTCCCGCC20.6493506493506493No Hit
CCTCCAAAGGAACGCAGTTCCTCACCAGCAACAGAACAAAGCTGGATGGA20.6493506493506493No Hit
CGTTTTTCAGGACCTGTTGGTACCAGATCGGAAGAGCGTCGTGTAGGGAA20.6493506493506493Illumina Single End PCR Primer 1 (100% over 26bp)
CCCTTGGGATTGCCGGTGGTGCCCGAGGTATAGCACAGGCTGGACGCTGT20.6493506493506493No Hit
CGCTAGCGCTGCCTCACCGAGGTAGCGCTACTCGGCGTGCGCAAGCAACT20.6493506493506493No Hit
GCCTGCAGCGCGGCGCGGGTCACGGCCAGCACGGCCAGCACGTCGTTGCC20.6493506493506493No Hit
AGCGTGACCTGGGCGAGCAGGCGTTCCCCTATGTGTTCCTCGACGCCACC20.6493506493506493No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG20.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
GTGTAGATCTCGGTGGTCCCGATCTCCCATGTACTCTGCGTTGATACCTG20.6493506493506493No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT20.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC20.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT20.6493506493506493Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTAGGAAAGAGTGTAGTCTGGTTCCCATGTACTCTGCGTTGATACCAC20.6493506493506493Clontech SMART CDS Primer II A (95% over 21bp)
CATAAAAAAACAAATTAAATTGCAAGCACTGTAAAAAAAACACATAACAT20.6493506493506493No Hit
GTCCAAGGAGAACTCGAATGAATCCGAATGACTACCTGGTGCTCTCGGCG20.6493506493506493No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCTGTCTCTTATACACATCT20.6493506493506493Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT20.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATACAC20.6493506493506493No Hit
CTATAACGGCATGCGCGGCCTCCATCACAGAGAACCCGGTGTCGTCGGCT20.6493506493506493No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATCTGTCTCTTATA10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
ACTCAGAGTACATGGGAAGCAGTGGTATCAACGCATAGTACAGGGAAGCA10.3246753246753247Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTACTTTTTTTTTTTTGCGTTGATACCACTGCTTCCCATGTACTCCTG10.3246753246753247Clontech Universal Primer Mix Long (95% over 21bp)
GCAGAGTACATGGGTGTCGGCCCGAGACTACACTCTTCCCTACACGACGC10.3246753246753247No Hit
GAGTACATGGGTCGCGACACCGAGATTACACTCTTTCCCTACACGGAGCC10.3246753246753247No Hit
GTACTGGGTGGCGACAGATCTCACTCTTCCCTACACGACGATCTCACTGT10.3246753246753247No Hit
AGGCTTGGCTGACGAGGGTGAGTTCGCCGCCGGTGGCCCTGTCTCTTATA10.3246753246753247No Hit
GTATCAACGCAGAGTACATGGAAGCATTGGTATCAACGCCTGTCTCTTAT10.3246753246753247No Hit
GATCTACACTTTTCCCTACACGACGCTCTCGTTTTTCAGGACCCTGTCTC10.3246753246753247No Hit
AGGTTGTTCGAGGCCGGGCTGCCGTTGTGCGGCACCGTAGTGATCAGCTC10.3246753246753247No Hit
CTATAAAGACAATGCACACATATGTTTATTGCAGCACTGTTCAACAATAG10.3246753246753247No Hit
CGTAGCCCTGCACCGCGTACACGTCGGGCTGCAGCTTGAACGACGCGGCG10.3246753246753247No Hit
CTTTTCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTCTGCAGGTA10.3246753246753247Illumina Single End PCR Primer 1 (100% over 27bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCTG10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
CCCAGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATA10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
ACCTGATCGCGCTGTCGTTCGTCCGGTCACCGGCCGACATCGAACTCGTG10.3246753246753247No Hit
GTCTACTCTTTCCCTACACGACGCTCTTCCGATCTATCCCTTTTTTGGCC10.3246753246753247Illumina Single End PCR Primer 1 (96% over 32bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTAT10.3246753246753247Clontech SMARTer II A Oligonucleotide (100% over 22bp)
CCTCGGCCGCACCCTGCAGCCGCACCCGCCGACGCAGGAGGCGCTGCGCA10.3246753246753247No Hit
GTACACGGGCGGCGGCCTGCTCGAAGTCGGCGGCTACCTGGGCACGCAGG10.3246753246753247No Hit
GTCGAAGAGCATCCCCCGGACCCTGGAAACCAGCCGATCGGCTGCCCCAG10.3246753246753247No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGAAAAATGCTTTTAAACCA10.3246753246753247No Hit
ATCTGAGGCAGCGCGTGTAGGGAAAGAGTGTAATCGGTGCGCCAGCCCAT10.3246753246753247No Hit
CACAAACATCACAAAGCGGTTTCTGAGAATGCTTCAGTTTAGTTTTTCTG10.3246753246753247No Hit
GTTCGAACACTGAGTACATCCGGCGAGAGTCGGCGGCCTCAAAGTGGGTG10.3246753246753247No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTCTGTCTCTTAT10.3246753246753247Clontech Universal Primer Mix Long (96% over 25bp)
AAACAAATAATAGTACAAACAATCCCAAAAAAGAGATAAAACTCAATCAC10.3246753246753247No Hit
GTACCTGGGAAGCAGTGGTATCAACGCAGAGTACAAGGGAGATACGGGAC10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCTGTCTC10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
TCCCAGGTGACCGTCGTTGCGGAGTGCGCCGAGGTGCACGGCAGGGTGCG10.3246753246753247No Hit
GCAGAGTACATGGGTGATACGGCGACCACCAGATACCTTTTCCCTACACG10.3246753246753247No Hit
ATTGAGAGCTGTTGTGCCATTTCTGTACACATAGATTCATTGACTTACCC10.3246753246753247No Hit
CCGCAGGTGCGCCCGGATCCGGGCGTGCAACTCGGCGAGACGGACCGGCT10.3246753246753247No Hit
TATCAACGCAGAGTACATGGGGAAGCAGTGGTATCAACGCAGAGTACATG10.3246753246753247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATAGCACTGCTTCCCATGTACTCTGCGTTGATCTGTCTC10.3246753246753247Clontech Universal Primer Mix Long (95% over 23bp)
GATCGTCACCCACAAGGTCATCCACGCACTCGATGGTCACCAGTGCGTCG10.3246753246753247No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCT10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
GCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTTCACCCATG10.3246753246753247Illumina Single End PCR Primer 1 (97% over 41bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACACA10.3246753246753247Clontech Universal Primer Mix Long (95% over 21bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGTCT10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAGTACTCTGCG10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
GTGTAATCTCGGGGTGCTCCCATGTACTCTGCGTTGATACCACTGCTTCC10.3246753246753247Clontech SMART CDS Primer II A (96% over 26bp)
GCGCAAAGTCCACTGCGTCCTGCGCCATCTGGCGATACGACGTCAGCTTG10.3246753246753247No Hit
CGACAAGGGCAGCCTCACCGTGACCGGCACCATCGACGCCAGCGGCGAGC10.3246753246753247No Hit
ACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA10.3246753246753247Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCGTTGATACCACTGCTGTGGTATCAACGCAGAGTACATGGGAAGCAGTG10.3246753246753247Clontech SMARTer II A Oligonucleotide (95% over 23bp)
AAATATTTAATCTTCCTAACGATATAACAGCACAGCTATTCCTCAAATCC10.3246753246753247No Hit
CAGACTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGACGGCCG10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
ACACGACGCTCTTCCGATCTCAGCAATTCCAGTCCTTGCATCATGAGATC10.3246753246753247No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTTCAGGTATCGCGTATG10.3246753246753247No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGATTCGGCGA10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGCTGGGAATTCACGTCAGTTTCTGCACTGAAACTCTCAAGATCAAT10.3246753246753247No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATAC10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
GTTTGCGCCTTTGTTTGTGATCGGTTTGCTCGGTTTGGGGATGTATAACG10.3246753246753247No Hit
GAGTACATGGGAAACTGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.3246753246753247No Hit
CCTGAACATCGTGGCGCCGCTGCTGACGGGCGAGGCGGGTTCGGTGAACC10.3246753246753247No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.3246753246753247Clontech Universal Primer Mix Long (100% over 23bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA10.3246753246753247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTAGGCTTCGCCGCCGAGAGCGAGAACCTCGCCGAACATGCCAAGGCC10.3246753246753247No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTAAACACAGCTT10.3246753246753247No Hit
TCCACTGACAGATGTCGCTTATGCCTCATCAGACACCATGGACACAACGT10.3246753246753247No Hit
GATGCAGACTCGGGTCCCGATCACCCATGTACTCTGCGTTGATACCACTG10.3246753246753247Clontech SMART CDS Primer II A (95% over 23bp)
AGGGCATCCCGCTCGGTGGCTTCATCCGCATCGTCGGCATGATCCCGCCG10.3246753246753247No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.3246753246753247Clontech Universal Primer Mix Long (95% over 22bp)
TCCCCGCACCGCTCGCCGAGCCAGGCGGTGAAGTCCGGCCACCAGGAGCC10.3246753246753247No Hit
GTAGGGAAAGAGTGTAGATCTCGGTGTCGCCGTACATCCCATGTACTCTG10.3246753246753247Illumina Single End PCR Primer 1 (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTATA10.3246753246753247Clontech Universal Primer Mix Long (95% over 21bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTAAG10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
CCGGAAGAACTGGATCGGGGCCGAGGAGTTCCCGTTCACCACGGTCGCGG10.3246753246753247No Hit
AAACAACAAAACACTCTACATAAATCAAAAAGAAAATAATTGATATTATC10.3246753246753247No Hit
TCCCTTACTCTGCGTGAATACCATGCTCCCATGTACTCCTGTCTCTTATA10.3246753246753247No Hit
ATCAACGCAGAGTACATGGGAAGCAGTCGAAACAACGCAGAAAACAAACA10.3246753246753247No Hit
GTACATGGGTGTACGGCGACCACCGAGATCCTGTCTCTTATACACATCTC10.3246753246753247No Hit
TTCCAACACATCGACAGCCCATTTGACTTCACGGCGTCCTCACAAACCTG10.3246753246753247No Hit
GTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGAGTACATGG10.3246753246753247Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAGAAAAAAAAAAAAAAAAAAA10.3246753246753247Clontech Universal Primer Mix Long (95% over 22bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAGAGTACTTTT10.3246753246753247Clontech Universal Primer Mix Long (95% over 22bp)
ACCCGCAGCCCGGTGAAGACGATGCGCTCAAGGCCGAGTCGATCAAGCTC10.3246753246753247No Hit
ACCAGCGACACCTCGGTGCCCGAGCGGGTCAGCGCGGCGTGCGCCGAGGA10.3246753246753247No Hit
ATCAACGCAGAGTACATGGGGCCTGTTGGTACCAAAAAAGGGATAGATCG10.3246753246753247No Hit
GGTCCTGAAAAACGAGCGTCGTTAGGGAAGAGTCCGCCCATGTACTCTGC10.3246753246753247No Hit
GTTCAGGCACGGCGCCGGGCAGGTCGAACGCGCTGCGGTCGATCAGGGGG10.3246753246753247No Hit
CCCTACACGACGCTCTTCCGATCTATCCCTTTTTTGGGCCTGTTGGTACC10.3246753246753247Illumina Single End PCR Primer 1 (100% over 24bp)
GTGTATAAGAGACAGCTCGACGAGCTCGCCGACCGCTCGGTGGCCGTCGT10.3246753246753247No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
GGTCCTGAAAAACGAGAGAGCGTCGTGTAGGGACTCGGGCCGCCCCATGT10.3246753246753247No Hit
GTCGCCGCACTGCAGGACGCGGCGAAGTCCCCTGGCCGGCTTCGGCGGGA10.3246753246753247No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACCCAGAGTACATGGGAA10.3246753246753247Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTT10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTACTCCTG10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
CATCTACAATCATAACATACAACACCATAATCATATAAAAATATCCGAGC10.3246753246753247No Hit
CTAACACTACAAGACGATAAAACAAAATATCTGTCTCATATACACATCTC10.3246753246753247No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGT10.3246753246753247Clontech SMART CDS Primer II A (96% over 26bp)
CCGGTGATGATCGGGGCGTTCGGATCTTCGCCTTCGCGGCCGACGAAGGT10.3246753246753247No Hit
CCACTGCTTCCCATGTACTCAACGCAGAGTACATGGGAAGCCGTGGTATC10.3246753246753247No Hit
GAGTAGGTCGCGTCGGAGTAGTAGCGCTCCAGCTCCGCGAGCGGCTCGTC10.3246753246753247No Hit
GTATCAACGCAGAGTACATGGGAAAGCGACCCGGACTACACTCTTTCCCT10.3246753246753247No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTACGGGCCCGA10.3246753246753247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
TCCTGGTAGAGCGGCGGCGTCCACGCTTCCAGCGCATCGGCCGGCTTGCC10.3246753246753247No Hit
GTACATGGGAAGCAGTGGTATCAACGCTGAGTACATGGGAAGCAGTGGCT10.3246753246753247Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACATGGGATGATGGGACACCGAGATCTACCTCTTCCCTACACGACGCT10.3246753246753247No Hit
GATGAGGGCGTACTCGATGGAGTACCGGCGGCCGGTCTTCCGCGCGTAGG10.3246753246753247No Hit
CGACGGGGGCCTCGGCATCGACACCGCCTCCTGGGCCCTGTCTCTTATAC10.3246753246753247No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGTGGT10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCATCCTTCAGGTATCTCGTATG10.3246753246753247No Hit
GTAGGAAGATGGATCTGGCCACATCCCATGTACTCTGCGTTGATACCACT10.3246753246753247Clontech SMART CDS Primer II A (95% over 22bp)
CTCGGGCACCGTGGGCATCCGCGTCCCCCTCGCCGAGTCGCTGTGCGCGG10.3246753246753247No Hit
ATACGCGACCACCGAGACTACACTCTTTCCCTACACGACCTGTCTCTTAT10.3246753246753247Illumina Single End PCR Primer 1 (95% over 22bp)
GGGAAGCCGTGGTATCAACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.3246753246753247No Hit
GTAAATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT10.3246753246753247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAGTCGAAGCCGCCGACGTCGCCGAACAGTCCGCCGCCGTCCCCGAAGT10.3246753246753247No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGTCGGCCGAGATCTACACA10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAAGAGTGTAGATCTCGGTGGCCTACCCATGTACCTGTCTCTTATAC10.3246753246753247Illumina Single End PCR Primer 1 (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGCTGTC10.3246753246753247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGCGCCGTATCACCCATGTACTCTGCGTTGATACCACTGCTTCCCATG10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGAACACACCTCTACACGACGCTGTCCCAAGTCTGTCTCTTA10.3246753246753247No Hit
CTCTGGATGTCCTTGAAAACTAAACGCAGGCTTATCGGTCAGCTCAATGC10.3246753246753247No Hit
ATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTC10.3246753246753247Illumina Single End PCR Primer 1 (100% over 37bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGCTGTCTCTT10.3246753246753247Clontech Universal Primer Mix Long (95% over 23bp)
GAACACACCCACGATTCAGTACGTTGGTTTATTTGGCGCAACAAATACCA10.3246753246753247No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10.3246753246753247No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTTCCTGTCTCT10.3246753246753247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAATTACTCTGCGTTGATACCACTGCT10.3246753246753247Clontech SMART CDS Primer II A (96% over 25bp)
GGCCGTATCATTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGGACGGCGCACCTAGATTACAAAACCCTACACGACGCTCTTCCGA10.3246753246753247Illumina Single End PCR Primer 1 (95% over 22bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGTCTCTTATAC10.3246753246753247No Hit
GCGCTGATGTGCGCCCGCCCGATCGCCAGGGTGCCGCTCAGCGCGAGCCA10.3246753246753247No Hit
ATACATTTTAAGCAAACAAAACAACAAAAAATACATAGAAAAAAAACACT10.3246753246753247No Hit
ACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGGTACCTGCAGAACT10.3246753246753247Illumina Single End PCR Primer 1 (100% over 33bp)
AAAAAAAAAAAAAAAAAAAAGTACTATGCGTTGATACCACTGCTTCCCAT10.3246753246753247Clontech SMART CDS Primer II A (96% over 26bp)
CCTCCGGACGGTGACCACCGTCTCGGTGCGGCGGGCGGCCGGGTCCACGC10.3246753246753247No Hit
CAGGAGCTGTCACAAACCCTAAACGCCAGCCAGTCATGGCAAATGCTTTC10.3246753246753247No Hit
TGGGAAGCAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAGTA10.3246753246753247Clontech Universal Primer Mix Long (95% over 21bp)
GGGCCTGCCCGAGTACGTCGCCGACGACGCGCCGCTGGAGAACACCGACG10.3246753246753247No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTAAAAAAAAAAAAAAAAAAAAA10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
GTGTAGGAAAGAGTGAATCTCGGTCGCGACCCATGTACTCTGCGTTGATA10.3246753246753247No Hit
GATCTACACTCTTTCACTACACGACGCTCTTCCGATCCTGTCTCTTATAC10.3246753246753247Illumina Single End PCR Primer 1 (97% over 37bp)
CCCGGAAAAATTTGACGCGGCCGTGCACTCCATGGCCGATTATCAAAACT10.3246753246753247No Hit
ACATGGGACCGACCACCAGACAACACTCTTTACCTAACCGACGCTCGTTC10.3246753246753247No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGT10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTACCAACGCAGAGTACATGGGGCAGTGGTATCAACCCAAAAAACTG10.3246753246753247No Hit
TGCTGGGCCAGTTCTCCGCTTTCCCGGGTCAGCACAAACCCTTTGGCCAC10.3246753246753247No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCAGAGTACTTTTT10.3246753246753247Clontech Universal Primer Mix Long (95% over 21bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
CCAAAGCTGCCAGCTCCGGGTACCGTGCCGGAGGCACCAGGTATTTCTCC10.3246753246753247No Hit
CTTCCTGGAATGGCCGTCCCCATGGATCCTGAACCAGGAAATTGTCTACC10.3246753246753247No Hit
ATCTCGGTGGTCGCCGTATCATTCCCATGTACTCTGCGTTGATACCACTG10.3246753246753247Illumina Single End PCR Primer 1 (100% over 23bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
GATACCATGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACA10.3246753246753247No Hit
GTACTTGGCGGCCTGAATTGCGTACTGGATCATGTCCGGCTGCTTCCACA10.3246753246753247No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT10.3246753246753247Clontech SMART CDS Primer II A (100% over 26bp)
CAGTCCCCGCATCATGGTCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGA10.3246753246753247Illumina Single End PCR Primer 1 (100% over 29bp)
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAGTA10.3246753246753247Clontech Universal Primer Mix Long (95% over 22bp)
GGGTTTTGACCACCACGAGATACCTCTTTCCCTACACGACGGAGCTGCCT10.3246753246753247No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT10.3246753246753247Clontech SMART CDS Primer II A (100% over 26bp)
GTCGGGTATAGCGGCCATCTGGTATGCTAATCTTGAAGACTTCGTGAAGA10.3246753246753247No Hit
GTCGTGTAGGGAAAGAGTGTAGATCGTGGTCCTATCCCATGTACCTGTCT10.3246753246753247Illumina Single End PCR Primer 1 (96% over 25bp)
CATGAGGGGCTCTACCCTCATGACCTAATTATTTCCCAAAGACCCCACCT10.3246753246753247No Hit
GCAGAGTACATGGGTGTCGGCCCGAGACTACACTCATCCCTACACGACGC10.3246753246753247No Hit
GCGTTGATACCACTGCTTGCCCATGTACTCTGCGTTGATACCACTGCTTC10.3246753246753247Clontech SMART CDS Primer II A (96% over 26bp)
ACACCACGCTCTTCCGATCTGTTCTGGTATCGACAAGACCTGGGACAGAT10.3246753246753247No Hit
ATCAACGCAGAGTACATGGGGAAGCAGTGGTATCAACGCAGAGTACATGG10.3246753246753247Clontech Universal Primer Mix Long (96% over 27bp)
GTGGTGGACCTTGGTGTGGTGGCGTTCGCAGAGGAGCGCTGAGTTGTCGA10.3246753246753247No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGCG10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
GTGTAGGGAAAAATGTAGATCTCGGTGGCGCCGCATTCCCATGTACTCTG10.3246753246753247No Hit
GTGGAACTGTATTTGCGCGGCATTATCATCCTGCACCGAATAAGAAACTT10.3246753246753247No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGTCGGA10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCGATCTCTGGTACCGACAGAATCTGAGGCAAGAGCGTCGTGTAGAA10.3246753246753247No Hit
TCTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATC10.3246753246753247No Hit
GATCTACACTCTTTCCCTACACGACGCTCTTCCGATCCTGTCTCTTATAC10.3246753246753247Illumina Single End PCR Primer 1 (97% over 37bp)
CGGGTGACGAAGCTGCTGCTGCGGGGGTGCGCTGCGCGATGGACGTTTCC10.3246753246753247No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACAAACCGTTTTT10.3246753246753247No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAGTA10.3246753246753247Clontech Universal Primer Mix Long (95% over 21bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTGTATACAAATCCTA10.3246753246753247No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGTTCGGA10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT10.3246753246753247No Hit
GTCGAGGCTGAAGCCGCACAGGCTCATCACGAACAGGGTGGCGGCCAGCG10.3246753246753247No Hit
AACCAAAATGGCCACGAGAGTGACCTCTGGTCGTCGTCACTGCTGTGCTC10.3246753246753247No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGATACGGC10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
GTAAAAGCGTACAGAGAAAACACGCCACCCGGCGGACGATTGTCCGCCGG10.3246753246753247No Hit
CTGCACCTCGCCGGCCGGTCCGACGTCCCGGTAGCGGCCGGCGCCCGCGC10.3246753246753247No Hit
TATCAACGCAGAGTACATGGGTCGGACACCGAGATCACTCTTTCCCTACA10.3246753246753247No Hit
GAATAGGCACAGTTACCTCTCCCTACACCTGTCTCTTATACACATCTCCG10.3246753246753247No Hit
CTCTTTCCCTACACGACGCTCTTCCGATCTCTGGTACCGACAGAATCTGA10.3246753246753247Illumina Single End PCR Primer 1 (100% over 30bp)
CCCATGTACTCTGCGTTGATACCACTGCTTGCCATGCTGTCTCTTATACA10.3246753246753247Clontech Universal Primer Mix Long (96% over 28bp)
ATCAACGCAGAGTACATGGGTGACGGGCCACCGAGACTACACTCTTTCCC10.3246753246753247No Hit
GCGTGCTGCCTCGCAGGGCCTTGCGCGCCAGCAGGTCGATCTGCAGGTCG10.3246753246753247No Hit
ACACTCTTTCCCTACACGACGCTCTTCCGATCTGTCCCAGGTCTTGTCGA10.3246753246753247Illumina Single End PCR Primer 1 (100% over 33bp)
GAGTACATGGGAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT10.3246753246753247Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA10.3246753246753247Clontech Universal Primer Mix Long (95% over 21bp)
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAGTACCTGTCTCTTATA10.3246753246753247No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTAT10.3246753246753247Clontech Universal Primer Mix Long (95% over 21bp)
GTGGTATCAACGCAGAGTACATGGGATGTCGGCGACACCGAGATTACTCT10.3246753246753247No Hit
GATCTACACTCTTTCCCTACACCACGCTCTTCCGATCTGTCCCAGGTCTT10.3246753246753247Illumina Single End PCR Primer 1 (97% over 38bp)
GCTGCGGCCGCTCGAGCTGACCTTCGCGCGGTACGAGGTGCTGGTGCTGC10.3246753246753247No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT10.3246753246753247No Hit
GGTTGGATGGGGCGGTCTTGGGAGGAGGGCGGACACCGTCGGCTGCGGCA10.3246753246753247No Hit
CCATCACGCTGCGGACGCAGCGTGATGGTGCATTGCAATAGAATTTTGCG10.3246753246753247No Hit
GTGTAGGGAAAGAGTGTAGATCTCGGTGGTGCCGACCCATGTACCTGTCT10.3246753246753247Illumina Single End PCR Primer 1 (96% over 30bp)
CCCATGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTCCGA10.3246753246753247Clontech SMART CDS Primer II A (96% over 25bp)
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCTGTCTCTTATAC10.3246753246753247Clontech Universal Primer Mix Long (96% over 25bp)
ATGGTAAGCAGTGGTATCAACGCAGAGTACATGGGATCCGCCGTCTACAC10.3246753246753247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCATGGTATCAACGCAGAGTACCTGTC10.3246753246753247No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAAAAAAT10.3246753246753247No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTT10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
GTCTCCGGCGACGTACCAGAATCGTTGCGCGGTAAGAAACTTATCGCCCT10.3246753246753247No Hit
GCGGCCCAGTGGTGCATCTGGCGGACAAACAGGCCGCCGCGCACGTCGAA10.3246753246753247No Hit
GAGGAACAGTTCGCCGTCGCGCACCAGCAGGTTGGCCGGCTTGATGTCGC10.3246753246753247No Hit
TGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATC10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTCGTTTATAACACTGCT10.3246753246753247No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)