FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003652792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003652792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT82.5974025974025974Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA72.272727272727273Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG61.948051948051948Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA41.2987012987012987Clontech Universal Primer Mix Long (96% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41.2987012987012987No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG41.2987012987012987Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC41.2987012987012987Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT30.974025974025974Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT30.974025974025974Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCCAGGACGGTGGCGATGGCGTTGGTCAGTCCGCGGTGGGAGTCGGAGA20.6493506493506493No Hit
GATCCCGTCGGCAATGATTTCAACGTAAGAATCGCCTAAAAAGCTGTAGC20.6493506493506493No Hit
GTATCAACGCAGAGTACATGGGAGATCGGGACCACCGAGATCTACACCTG20.6493506493506493No Hit
CGTGTAGGGAAAGAGTGATCGGTGTCCGACCATGTACTCTGCGTTGATAC20.6493506493506493No Hit
CCACCTCAGCAGCTGCCACAATCATCACGAAGAAAGCTCCGACCTGTCCG20.6493506493506493No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTGGGAAGCA20.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
GCGCAGTTGCTTGCGCACGCCGAGTAGCGCTACCTCGGTGAGGCAGCGCT20.6493506493506493No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT20.6493506493506493Clontech Universal Primer Mix Long (100% over 23bp)
CTCCCGTAGCTCAGAGTAATTTGATCGTCTGAAGCCTTCTTCTCTCAGCT20.6493506493506493No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCCTGTCTCTTATAC20.6493506493506493Illumina Single End PCR Primer 1 (97% over 37bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG20.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
GGGCCACATGGGTGACGGTGGCGCCGGCCGCGCGCGCCACGCCTGCCGTG20.6493506493506493No Hit
GGGCTTCAACCTGTACACGATCGTCATCGGCGCCCAGTGCCTGCACGCGA20.6493506493506493No Hit
ACACTCTTCCCTACACGACGCTCTGTCCCAAGTTCTGCAGGTACCAGAGA20.6493506493506493No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCTCTTATACAC20.6493506493506493No Hit
CTCTTTCCCTACACGACGCTCTTCCGATCTGGTACCAACAGGTCCTGAAA20.6493506493506493Illumina Single End PCR Primer 1 (100% over 30bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT20.6493506493506493Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTATACACATCT20.6493506493506493Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTAG20.6493506493506493Illumina Single End PCR Primer 1 (100% over 22bp)
TTCGTTGACCGCGCCATCACGAGCGCCGTCGCGGAACGACTGCATCTCCC20.6493506493506493No Hit
CGTCCAGCCTGTGCTATACCTCGGGCACCACCGGCAATCCCAAGGGCTGT20.6493506493506493No Hit
TACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCT20.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
ATCTGCGACCGCACCACGCTGATCCGCGCCGCCGTGAACGATGTCAAGCT10.3246753246753247No Hit
CAGTGGTATCAACGCAGAGTACATGGGAATGCGGCGCCACCGAGATCTAC10.3246753246753247Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GGGAAAGAGTGTGACTCGTGGTCGCCGTTCACCCATGTACTCTGCGTTGA10.3246753246753247No Hit
GTATCAACGCAGAGTACATGGGATACGCACAGTCTACTCTTTCCCTACAC10.3246753246753247No Hit
TGGGAATGTCGGGGGGCCCCCCCATGGAAAAACAATTAACAAAAAACGGC10.3246753246753247No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG10.3246753246753247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTT10.3246753246753247Clontech Universal Primer Mix Long (95% over 23bp)
GCCTAATGCGCGTGTTGAGTTCGTCTAGGTCGACGCACTGGTGACCATCG10.3246753246753247No Hit
GCGTTCCAGGGCGGCATCTTTTTCAATGTATTTGCGGTACTCGTCCAAGG10.3246753246753247No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGCTATCAACGCAGAGTCTGTCTC10.3246753246753247Clontech SMARTer II A Oligonucleotide (95% over 23bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTGGGCTGTCTCTTATA10.3246753246753247Clontech SMART CDS Primer II A (100% over 26bp)
GAGTACATGGGAGCATGGTATTCACGCAGAGTAAGGGACTGTCTCTTATA10.3246753246753247No Hit
GTTTCACCCTGATCGAAGTGGTGGCATCCCTGCTGATCACCTCCATGCTG10.3246753246753247No Hit
GAAGAGAGAAACTGAAAGGCAGAACTGAGAAAGCTCTTTGCTCATTGATC10.3246753246753247No Hit
AGCGCGGCCAGCCTGCCCGGACCCTCGGCGTCGAGCGTGGACGCGTAACT10.3246753246753247No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTAAAACAAAGA10.3246753246753247No Hit
GTACTCTGCGTTGATACCATGCTTCCCATGTACTCTGCGTTGATACTGTC10.3246753246753247No Hit
GTGTAGGGAGAGGTAACTGTGCCTATTCCTGTCTCTTATACACATCTGAC10.3246753246753247No Hit
TCTCCACCGTGGGTCTGGTGCCGGCGATCCGGCGGCTCACCGAGGAGGGG10.3246753246753247No Hit
CATCATGACTGTCGAGGCTGCCGACCACAAAGCCGCCGCCATGATAAAAC10.3246753246753247No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAA10.3246753246753247Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTCGTGTAGGGAAAGAGTGTAGTCTCGGTGGTCGCGTATCTGTCTCTTAT10.3246753246753247Illumina Single End PCR Primer 1 (95% over 22bp)
CTACAGAGGGATTCAAAACGCTGCTGGTTTCTGGGGACCGTGACGCCATG10.3246753246753247No Hit
GTATCAACGCAGAGTACATGGGTGATACGGCGACCCCGAGTCTACACTCT10.3246753246753247No Hit
GTCTGATGGCGGACCACGGGCGTATAGGCGTTATACATCCCCAAACCGAG10.3246753246753247No Hit
CCGTATCACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCC10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
GTGGACCTCTTTGAAGATTTCTTTGGAAACGGGAATATTTCCACAGAAAA10.3246753246753247No Hit
CCACTGCTTCCCATGTACTCAGCGTTGATACCACTGCTTCCCATGTACCT10.3246753246753247Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTC10.3246753246753247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCATGGTATCCTGTCTCTTATACACA10.3246753246753247No Hit
AGATTACACTTTTCCCTACACGACGCTCTTCCGATCTCTGGTACCTGCAG10.3246753246753247Illumina Single End PCR Primer 1 (100% over 27bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTC10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAGTATAAAA10.3246753246753247No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTGCGTTGATAACACTGCTTCCCAT10.3246753246753247No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAATATGCGTTTATAA10.3246753246753247No Hit
GTACATGGGCTGGCGCACCGATTACACTCTTTCCCTACACGCGCTGCCTC10.3246753246753247No Hit
GGCCCAGGAGGCGGTGTCGATGCCGAGGCCCCCGTCGCTGTCTCTTATAC10.3246753246753247No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTTATA10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGCT10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGTAACTC10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
GTCGGTACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
ATGGCAAGCAGTGGTATCAACGCAGAGTACATGGGATGTACGGCGACACC10.3246753246753247Clontech Universal Primer Mix Long (96% over 28bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGTCTCTTATACACA10.3246753246753247Clontech Universal Primer Mix Long (95% over 21bp)
GAGTACATGGGTGTACGGCGACCCGTCTTTCCCTGCTGGTACCAGTAGAC10.3246753246753247No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCTCTTATAC10.3246753246753247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCGTACCCATGTACTCTGCGTTGATACCACGGCTTCCCATGTACTCTGC10.3246753246753247Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA10.3246753246753247Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTCATTGGGGGATGGGCTATCGTGGCTTTTGTGACTTGTTGGCTTGTTGC10.3246753246753247No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTATTTGGGTTTTAACCAATGG10.3246753246753247No Hit
CTCCCTACACGACGCTCTTCCGATCTCATGATGCAAGGACTGGAGTTGCT10.3246753246753247Illumina Single End PCR Primer 1 (96% over 26bp)
ACTTGGGACAGCGTCGTGTAGAGGTGTGTTCCCCATGTACCTGTCTCTTA10.3246753246753247No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCTAGCAATGTGAAAGTGACGCATCGTTCGTTATTTGATGGCTCAAA10.3246753246753247No Hit
AGTACCTGCTGGTCACCGAGTTCATCGCCGGCGCGAAGGAGGCCGGCGAC10.3246753246753247No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGACTACATGGGAAGCAGTGGTA10.3246753246753247Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAGTACTCCTG10.3246753246753247Clontech Universal Primer Mix Long (95% over 22bp)
CCCAGGGACAGTCGGCTGGCCGGAACTGTCGGTAGCGTCGGGCCACCTAC10.3246753246753247No Hit
CTGACCGGTCAGATAACCCGGGTGCTGGCTCTGGCCCCGCCGCTGGCCGA10.3246753246753247No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTAT10.3246753246753247Clontech Universal Primer Mix Long (95% over 21bp)
CAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTATACACATCTGACG10.3246753246753247Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GCGTTGATACCAATGCTTCCATGTACTCTGCGTTGATACCTGTCTCTTAT10.3246753246753247No Hit
GTACTTTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTGTCTCTTATA10.3246753246753247No Hit
GTTTGTGAGGACGCCGTGAAGTCAAATGGGCTGTCGATGTGTTGGAACTG10.3246753246753247No Hit
GAAAGTGTGTCTCGTGGTCGCTCCCATGTACTCTGCGTTGATACCACTGC10.3246753246753247Clontech SMART CDS Primer II A (95% over 24bp)
CCGAGATCTACCTTTTCCCATGTACTCTGCGTTGATACCACTGCTTACCA10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
TTTGTGAACTGTCATGGCGCTGGTGGGAGCACAGCAGTGACGACGACCAG10.3246753246753247No Hit
TCCCTGCTGGTACCAGTAGACAGTGAGATCGTCGTGTAGGGAAGAGTGAG10.3246753246753247No Hit
ATCCGCTGCCACCAGGGCTTCTTGTAGAAGACGCGGTCGCCGTCGGTGGG10.3246753246753247No Hit
CCGGAGAGCGCTCGAGCAGCTCCGCGAGGGCCACCACCGCCGGGCGCGGG10.3246753246753247No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTTCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTCTGCAGGT10.3246753246753247Illumina Single End PCR Primer 1 (100% over 26bp)
CAGTATCCGGATCGCGGCGCCACTTTGATTACCGTGCTGGCTGCTGACCA10.3246753246753247No Hit
TTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTCGTTTTTACCCA10.3246753246753247No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT10.3246753246753247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGTGAACGGCGACCACCGAGATCTACACTCTTTACCAACACGA10.3246753246753247Illumina Single End PCR Primer 1 (96% over 31bp)
CTCATGCACGAGTTCGATGTCGGCCGGTGACCGGACGAACGACAGCGCGA10.3246753246753247No Hit
GTCACGGCGGGAATCGCCGACCACCTGTGCCCGTGGCAGGCCTGCTACCG10.3246753246753247No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGATGTGGCCAGATCCA10.3246753246753247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAATATGGCGTTGATACCAA10.3246753246753247No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAT10.3246753246753247Clontech Universal Primer Mix Long (95% over 22bp)
ATGCTGATGCTCGGCGCGGATTTCTTCGACAAGGGCTTCAGCCGCTATGC10.3246753246753247No Hit
GTGTAGGGAGAGTGTAGTCTCGGCCGTCCCATGTACTCTGCGTTGATACC10.3246753246753247No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAAACTGC10.3246753246753247No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGGGACACGA10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
GATCTCGGTGGTCGCCGTACACCCATGTACCTGTCTCTTATACACATCTG10.3246753246753247No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA10.3246753246753247Clontech Universal Primer Mix Long (95% over 22bp)
GGCTCAGCTCGGCGCCCAATGCCGCGTCGCCGGTGGCGGCAACCGCCGCA10.3246753246753247No Hit
ATGGAAAGCAGTGGTATCAACGCAGAGTACATGGGAAAGAGTGTAGTCTC10.3246753246753247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGGTTTCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGATAGGACCACGATCTACACTCTTTCCCTACACGACCTGTCT10.3246753246753247Illumina Single End PCR Primer 1 (96% over 25bp)
CATGGCAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTATACA10.3246753246753247Clontech Universal Primer Mix Long (100% over 26bp)
CCCACACCGACGCTCCCCGGCTGCTTGGGAGCCCGGCGGCCGGGAACACC10.3246753246753247No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT10.3246753246753247Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGTAGGCCACCGAGATCTACACTCTTTCCCCTGTCTCTTATAC10.3246753246753247Illumina Single End PCR Primer 1 (96% over 26bp)
TTTTTTGGGTTGATACCACTGCCCCATGTACTCTGCGTTGGTACCACCTG10.3246753246753247No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTGTCTCTTAT10.3246753246753247Clontech SMART CDS Primer II A (96% over 25bp)
TCGTAACCCTACGTATCCCGCGGCAAGCCAACCGTGAGACAATTCCGGCC10.3246753246753247No Hit
GAACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCT10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
GTTCTGGCCGGGCTCGCCGCCCGCCTTGGCGTTGGTGCCGTGGCGCAGGT10.3246753246753247No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTGTGCGTTGATACCACTGCTTCCC10.3246753246753247Clontech Universal Primer Mix Long (95% over 21bp)
CTTCCGATCTATCCCTTTTTTGGGCCTGTTGGTACCAAAAAAGGGATAGA10.3246753246753247No Hit
GCCTCGAACCTGTTGCCGACAGGGAGGATATCGACTACCTGCTTGAACAT10.3246753246753247No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.3246753246753247No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAACTGTGCGTTTATACA10.3246753246753247No Hit
ATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAC10.3246753246753247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTTGATGGTGCCGGTGAGCACGTCGAGCGTGCCGCCCGACAGGTTGATG10.3246753246753247No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCCTG10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
GTGATGCGCCGCCGGTGCTGCGCTTCGACGACGGCCTCGTCGTCCCGGTG10.3246753246753247No Hit
GTGTAGGGAAAGAGTGAGATCTCGGTGTCGCCGATCACCCATGTACTCTG10.3246753246753247No Hit
TCATATAATATAATTGAATAAAAGACACACATATTATTAAAACACAAAAT10.3246753246753247No Hit
GGCCGGACCGGGTTCGGCGGCATCATCTCCGCGGCCAAGGCCCGGTGGCT10.3246753246753247No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA10.3246753246753247No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAATG10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
GGTCAACTACCGTGGCGACGGCAAAACCTGGAAGCCGCTGTCGGAAGCCG10.3246753246753247No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGACGCCCAT10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGTACGCGCCACCGAGATCTCACTCTTTCCCTACACGACGCTC10.3246753246753247Illumina Single End PCR Primer 1 (95% over 24bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTGCGTTTATACCACACCCATGTAA10.3246753246753247No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTAA10.3246753246753247No Hit
TGGTCGCGCTCCGCAATCGGCAACCCGCGGGCGGTCTCCGAGTGGCCGCA10.3246753246753247No Hit
CCAGCGTGAGCTTGGGGGCGCGCGGCAGCTCGCGCACGCCATTGGCATCG10.3246753246753247No Hit
ACACCCTTTCCCTACACGACGCTCGTTTTTCAGGACCTGTTGGTACCAGA10.3246753246753247Illumina Single End PCR Primer 1 (95% over 24bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGAGGTAGTTCGGTGTCGCCGAATCCCATGTACTCTGCGTTGATAC10.3246753246753247No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACACTGGTTTGAA10.3246753246753247No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTGGGAAGCAGTGGTAT10.3246753246753247Clontech SMART CDS Primer II A (100% over 26bp)
GGTAATGATACGGCGACCACCGAGATCTAACTTTTCCTACACGACGCTCT10.3246753246753247Illumina Single End PCR Primer 1 (96% over 26bp)
CTTCTCGTTCCTGGAGCTGCTGGCGCGGCTGCAGGCGCTGAACCGGCGCG10.3246753246753247No Hit
AGGGAAGAGGAGTCGTGGCATCCCATGTACTCTGCGTTGATACCACTGCT10.3246753246753247Clontech SMART CDS Primer II A (96% over 25bp)
GTATCAACGCAGAGTACATGGGAATAGCGACACCGAGATCTACACTTTTC10.3246753246753247No Hit
GTGATGCATTGGTATTTGTTGCGCCAAATAAACCAACGTACTGAATCGTG10.3246753246753247No Hit
CCCCCACCACCGGTTCGAGGGGGCGCGCCATCGCACGGAGCGCGTCACCG10.3246753246753247No Hit
TCCCTACACGACGCTCTTCCGATCTCATGATGCAGCAACTCCAGTCCTTG10.3246753246753247Illumina Single End PCR Primer 1 (100% over 25bp)
GTACTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT10.3246753246753247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA10.3246753246753247Clontech Universal Primer Mix Long (95% over 21bp)
TTTTGATAATCGGCCATGGATTGCACGGCCGCGTCAAATTTTTCCGGGCT10.3246753246753247No Hit
GAGTACATGGGTACGGCCACGAGATCTACACTCTTCTACACGGAGCTGCC10.3246753246753247No Hit
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA10.3246753246753247Clontech Universal Primer Mix Long (95% over 21bp)
CTGTCCCAAGTTCTGCAGGTACCAGAGATCGGAAGAGCGTCGTGTAGGGA10.3246753246753247Illumina Single End PCR Primer 1 (100% over 25bp)
GGTATCAACGCAGAGTACTGGGAAGCAGTGGTATCAACGCAGAGTACATG10.3246753246753247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTCCTGAAAAACGAGAGCGTCGTGTAGGGAAAAGTGTAGATCCTGTCTC10.3246753246753247No Hit
CAGTGATATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.3246753246753247Clontech Universal Primer Mix Long (100% over 23bp)
GTGTAGGGAAAGAGTGTAGTCTCGGTGGCCCGTCACCCATGTACTCTGCG10.3246753246753247No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGATCGGGA10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
GGTGAGATCTCGGTGTCCTCACCCATGTACTCTGCGTTGATACCACTGCT10.3246753246753247Clontech SMART CDS Primer II A (96% over 25bp)
TCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTCTGCAGGTACCAG10.3246753246753247Illumina Single End PCR Primer 1 (100% over 25bp)
CCCAGAAATTCACCTGGACCTACACCGCGCCGCATGCGACGAGCCAGTGG10.3246753246753247No Hit
GACTAATGTTTGTTTCCCCAACCCCCAAATTCCAATGTTGAAACTCTAAT10.3246753246753247No Hit
TCGTCATCTACGACGGTGCGTACCTGCCGCTCAACGGCGTCGAGATGTCG10.3246753246753247No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.3246753246753247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCTGTACTCTGCGTTGATA10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGACGGCGACACATCTACATCTTTACCAAAACGACGCGCCT10.3246753246753247No Hit
TGGAGCAGACCGCCGAGGAGCGCGCGCAGGGGCTCAGCCGGCTCCGCTGG10.3246753246753247No Hit
GATCGGAAGAGCGTCGTGTAGGGAAATGTAATCTCGGTGCGCCGTCCCCA10.3246753246753247Illumina Single End PCR Primer 1 (96% over 26bp)
AACACACTGGGCAGGGCTTGCACCGCTTCGTCGACCCGGCCGACGGCAAG10.3246753246753247No Hit
CTGCTTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGTGATCGGCGACCACCGAGATCTACACTCTTTCCCTACACGA10.3246753246753247Illumina Single End PCR Primer 1 (97% over 38bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTGTTA10.3246753246753247No Hit
ATCAACGCAGAGTACATGGGAATGATACGGCGACCACCGAGATCTACACT10.3246753246753247Illumina Single End PCR Primer 1 (100% over 30bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT10.3246753246753247Clontech SMART CDS Primer II A (100% over 26bp)
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCTGTCTCTTAT10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCGATCTATCCCTTTTTTGGCCTGTTGGTACCAAAAAAGGGATAGAT10.3246753246753247No Hit
GAGGCAAGCACCTCCGGGACCTTTCTGATACATAAAAAAACGCCTGCTCT10.3246753246753247No Hit
GTGAAGGTGCCGCGCGGGCTGTCGATCTTCGCCTTCTCGACGGCGGCCGC10.3246753246753247No Hit
CCCTACACGACGCTCTTCCGATCTGGGCCTCCAGTTCCTCCGGGATGAGG10.3246753246753247Illumina Single End PCR Primer 1 (100% over 24bp)
GCTAAAACCACTATCGGCGGCAACCTGGAGAATGGCGGCACGGTTCAAAT10.3246753246753247No Hit
ACACGACGCTCTTCCGATCTCATGATGCAAGGACTGGAGTTGCTGTAGGG10.3246753246753247No Hit
TAAATACCATCTGGCCAATCTTCATTCCTTCGCGCAACAATAGAATATGC10.3246753246753247No Hit
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.3246753246753247Clontech Universal Primer Mix Long (95% over 21bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGTATCAACGCAGAGT10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
GACTACACTCTTTCCCTACACGACGCTCTTCCGATCTATCCCTGTTGGTA10.3246753246753247Illumina Single End PCR Primer 1 (100% over 35bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTTTTTAACAAAGAT10.3246753246753247No Hit
GCTCCGTGTAGGGAAAGAGTGTAATCTCGGTGTCGCGACCCATGTACTCC10.3246753246753247No Hit
GGCCACCGGCGGCGAACTCACCCTCGTCAGCCAAGCCTCTGTCTCTTATA10.3246753246753247No Hit
GGGTGCCGTTGAGCTTGTGCTGCTGGACCTGGGTCTCCCCGATCTCGACG10.3246753246753247No Hit
GTATCAACGCAGAGTACATGGGTGCGGACCACGATCTACACTTTTCCCTA10.3246753246753247No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACTCTTGCGTTTAAACCACT10.3246753246753247No Hit
GTGTAGGGAAGAGTGTAGTCTGGTTCCCATGTACTCTGCGTTGATACCAC10.3246753246753247Clontech SMART CDS Primer II A (95% over 21bp)
GGTCTGGCTCGCGCTGAGCGGCACCCTGGCGATCGGGCGGGCGCACATCA10.3246753246753247No Hit
TCCCTACACGACGCTCTTCCGATCTATCCCTTTTTTGGTACCAACAGGCC10.3246753246753247Illumina Single End PCR Primer 1 (100% over 25bp)
CGGCGATCCGCTCGATGGGCGAGAAGGACGCGCCGGCCACGCCCACCTTC10.3246753246753247No Hit
GTACCTGTTCGGCCCCGAGATCGCGTTCCGCGCGCAGCCGCACGGCACCT10.3246753246753247No Hit
GATCGGAAGAGCGTCGTGTAGGAAAGAGTAATCTCGGTGTCGCCGACATC10.3246753246753247Illumina Single End PCR Primer 1 (96% over 25bp)
GCAGCTCCGTCGTGTAGGGAAAGAGGTATCTCGTGGTGGTCAAAACCCCT10.3246753246753247No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGACCGGTGGCGTCCCAT10.3246753246753247Illumina Single End PCR Primer 1 (97% over 37bp)
GTGTAGGGAAGAGTGTAATCTCGTGGTGCACCCATGTACTCTGCGTTGAT10.3246753246753247No Hit
GAGCAGCTCCGGTTGCAGCGACTCAAGCTCGGCCAGGCTCCCCGCGACCT10.3246753246753247No Hit
ATCTGTTCTGGTATCGACAAGACCTGGGACAGATCGGAAGAGCGTCGTGT10.3246753246753247No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG10.3246753246753247No Hit
TATCAACGCAGAGTACATGGGTCGCGACCGAGATTCACTCTTTCCTACAC10.3246753246753247No Hit
AAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.3246753246753247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCGGACTCGGCGAAGGCATGGATGGCGCTGGCGGCATGGACGGCACGGA10.3246753246753247No Hit
GTACATGGGATAACGACCACCGAGACTACATCTTTCCCTACACGACGCTC10.3246753246753247Illumina Single End PCR Primer 1 (95% over 21bp)
GAGTACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGCTG10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
CGCCGTAACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTGCCATGTACTCTGCGTTGAT10.3246753246753247Clontech Universal Primer Mix Long (96% over 28bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGAACGACCACGAGTCTACACTCTTTCCCTACACGACGCT10.3246753246753247Illumina Single End PCR Primer 1 (96% over 27bp)
TGGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.3246753246753247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCCCCCAGCTGGTGGCCCAGCTCGGCGGCGACGTGCTCGCCGTAGGCCG10.3246753246753247No Hit
GAGCACCCGGGACGGCGCCAGGCCGCACATCTCGGTGAAGGTGGAGAAGC10.3246753246753247No Hit
AGCTGATGCTCAATGAAGTTTTCACCTTGACAATAATCAGCAGTTTCTGT10.3246753246753247No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCGGTCTCTT10.3246753246753247Clontech Universal Primer Mix Long (96% over 26bp)
TCCTACAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGGACTGGAGTT10.3246753246753247No Hit
GTGTAGATCTGGTGTCGCGTTACCCATGTACTCTGCGTTGATACCACTGC10.3246753246753247Clontech SMART CDS Primer II A (95% over 24bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATAC10.3246753246753247No Hit
CCACGGTCCCGTCGACCACCGGCGGGAGGTCGGCCAGCCGCGGGTCGAGG10.3246753246753247No Hit
GTACATGGGTCGGCACCACCGAGATCTACACTCTTTCCCTACACCTGTCT10.3246753246753247Illumina Single End PCR Primer 1 (96% over 30bp)
GTACATGGGAATGATCGGCGACCACCGAGATCTACACTCTTTACCTACAC10.3246753246753247Illumina Single End PCR Primer 1 (97% over 35bp)
ACATGGGATCGCACGCTACCTTCCCTACACGACGCTCTTCCGATCTCATG10.3246753246753247Illumina Single End PCR Primer 1 (100% over 26bp)
GGTGGTCGACATCGAGCAGGTCTTCCACCACTGCGGCAAGGCGTTCATGC10.3246753246753247No Hit
GTGGTAAAGTTGTACCAATTGGGACGACGGTTGAAAACAATTTTCGATTA10.3246753246753247No Hit
ACGTCGAAGAGGGTCTCCCGCTCGCCGTCCCCGAGGTCGGCGAGACCGAA10.3246753246753247No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATCTGTCTCTTATA10.3246753246753247Clontech Universal Primer Mix Long (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGTAATGATACGCC10.3246753246753247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATCTGTCTCTTATAC10.3246753246753247Clontech SMART CDS Primer II A (96% over 26bp)
GCGCTGAACCGGCGGTCCGGCGGGAGGAGGTCGTCGAGGCCGGTGGCGGT10.3246753246753247No Hit
TCTTCACGAAGTCTTCAAGATTAGCATACCAGATGGCCGCTATACCCGAC10.3246753246753247No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)