FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003652799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003652799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1487
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA140.941492938802959Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG120.8069939475453934Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA70.4707464694014795No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT60.4034969737726967Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT50.3362474781439139Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC50.3362474781439139Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT40.26899798251513113Clontech Universal Primer Mix Long (100% over 23bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT40.26899798251513113Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG40.26899798251513113Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG40.26899798251513113Clontech Universal Primer Mix Long (96% over 26bp)
GTGTAGGGAAAGTGAGATCTCGTGGTCCCTAATCCCATGTACTCTGCGTT30.20174848688634836No Hit
CCACTGCCTCCTCCGCTTTCGAAGCGACATCAGCGAGGAGGCAGTCGTGA30.20174848688634836No Hit
CTTCCGATCTCTGGTACCGACAGAATCTGAGGCAAGAGCGTCGTGTAGGG30.20174848688634836No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC30.20174848688634836Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT30.20174848688634836Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA30.20174848688634836Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCTAGAGTCACCAAAGCCGCATTAAATCAGTTATGGTTCCTTTGGTCG30.20174848688634836No Hit
GGGCCGGAGAGGGGCTGACCGGGTTGGTTTTGATCTGATAAATGCACGCA30.20174848688634836No Hit
GTTAACAAGACGGCGCAGGTTGATGCTTCCAATAAACATGATCTTTCTGC30.20174848688634836No Hit
AATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATT30.20174848688634836No Hit
GCCCTGTTCTTCAGCACCGTGCTCGTTTTCCTCAGGAAGTCCCTGACACA30.20174848688634836No Hit
AATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTT30.20174848688634836No Hit
ATAAAATAATGTAAAATTTTTTTTAATATTTGAAATTTGTCTATAAGGAA30.20174848688634836No Hit
CAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAG30.20174848688634836No Hit
TAGCGGCGCAATACGAATGCCCCCGGCCGTCCCTCTTAATCATGGCCTCA30.20174848688634836No Hit
GCTACCATCTGTGGGATTATGACTGAACGCCTCTAAGTCAGAATCCCGCC20.13449899125756556No Hit
GGATTCAAAACCAGGCAATGTGGGTCCCGGGCACTCTTGACCAGTGCCTG20.13449899125756556No Hit
GACTGCGCACTGCTGGCGGTGCCCGCCGCCGAGCAACTGGCGGTTTCCAC20.13449899125756556No Hit
CAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCG20.13449899125756556No Hit
GCTCCTGGCTCATGGTGGGAATTCCTGCCTCAAGTCCTCCATCGGTTTCC20.13449899125756556No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA20.13449899125756556No Hit
AAGTGTAGATTCGTGTCGCCGTCATCCCATGTACTCTGCGTTGATACCTG20.13449899125756556No Hit
CAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCG20.13449899125756556No Hit
CCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGG20.13449899125756556No Hit
GTGTCAGTATCATCTATTAAACTTGAGAACCTCAGAATCAGGGCATTGCA20.13449899125756556No Hit
GACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATA20.13449899125756556No Hit
TTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGT20.13449899125756556No Hit
CTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCG20.13449899125756556No Hit
GCCCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTG20.13449899125756556No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA20.13449899125756556No Hit
GGCCAACTTAACGTGGGTTTCTGTATTCCCTGGTGTTGCTCTTAACCTGA20.13449899125756556No Hit
TCAATTAACTTCTACAGTGGAGACTACTTCTGGGACTGGAATATAAAAAA20.13449899125756556No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCAC20.13449899125756556No Hit
ATTTAGATCACTGATTTGTTTGAGTAAATTTTGTTTACGGTATGTGGTCA20.13449899125756556No Hit
TCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTCCC20.13449899125756556No Hit
CACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCA20.13449899125756556No Hit
CACTTTGGGAGGCCGAGGTGGGCGGATCACCTGAGGTCAGGAGTTGGAGA20.13449899125756556No Hit
GATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCA20.13449899125756556No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA20.13449899125756556No Hit
GTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGG20.13449899125756556No Hit
TCCTCGTTCATCGACGAGTAGCCCTCGACCTCGCGCTTGATCTCGAGCGC20.13449899125756556No Hit
CAGACGACAAGAAACCAGGGGAAAGAAAAATTAAGAGGCATGCCGGCGAT20.13449899125756556No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGTTGTTGCCATGGTAATCCT20.13449899125756556Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGC20.13449899125756556No Hit
ATACAGGACCCAAAAGGCTGAAAGGGGGCAGTGAAGTCGGACGGACTCAA20.13449899125756556No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA20.13449899125756556No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC20.13449899125756556Clontech Universal Primer Mix Long (96% over 26bp)
GTCGAAGGCCGTGCGGTGCCAGTCTCCGGAGAGCCGTTCCACCCGCAGCC20.13449899125756556No Hit
GGCTATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCC20.13449899125756556No Hit
CCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCA20.13449899125756556No Hit
GACTTGGCTTTTAGCAGAATTACAGTTTGCACCTGTATGGGTAAAACAGT20.13449899125756556No Hit
CCTTCCAGGTCGAAGAAGCGCAGCAGCAGATTCACCACCGTTGACTTGCC20.13449899125756556No Hit
CCTGAGGACTAGCATTAATTGACAGCTGACCCAGGTGCTACACAGAAGTG20.13449899125756556No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC20.13449899125756556Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT20.13449899125756556No Hit
GGCGGGGGACCGGCTATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGT20.13449899125756556No Hit
GTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGCTGATCTCCTCAT20.13449899125756556No Hit
TCACGGCCCCCACGGCCTGGGGACCCAGCTCAGCCACACACTTCTGGGAG20.13449899125756556No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCCTGTCTCTTATACAC20.13449899125756556No Hit
TGGTCCACCAGTGGAAGTGACTGCCGAGGAAGGGCGGTGAGGAGGGCGAT20.13449899125756556No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.13449899125756556No Hit
GACTATAGGTATGTGCACCACGCCTGGCTTAAAAGTCTTTTTGGTATTTA20.13449899125756556No Hit
CCCTAGCTCTATCCAAAGTTCATTTAAAAATGGGTTACTCCTGAGCTCTT20.13449899125756556No Hit
GAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCG20.13449899125756556No Hit
ATACATTTTAAGCAAACAAAACAACAAAAAATACATAGAAACTGAAACTA20.13449899125756556No Hit
GTCCACGGTCATCCAGCGCATCGCGGCGCATTTCACCGCCGAGCGCCTCG20.13449899125756556No Hit
GTATCAACGCAGAGTACATGGGCTATCTTGGATATGATATGGCGCACTAC20.13449899125756556No Hit
CATTCTCAAGCGCGCCAAGCCGGCGGCTGCGGCGCCGATGGAATCTGTGC20.13449899125756556No Hit
CCAATAAATGGTATTAAGTCAATTAACTTTCCATTTGGAAGGGATAAAAT20.13449899125756556No Hit
ATATTCATCCCTGTAGCATTGTTCGTTACATGGTCCATCATAGAATTCTC20.13449899125756556No Hit
GTACATGGGATGTCGGTGACCACCGAGATCTACACTCTTTCCCTACACGA20.13449899125756556Illumina Single End PCR Primer 1 (100% over 33bp)
GGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCC20.13449899125756556No Hit
GATCCTGAAGAACTGTGGACAGAAGTCCCTGGCCTATCTCACAGGTATAG20.13449899125756556No Hit
GTTGCAAACTATGATAAACTTACACTTGATGTTTGGACTGATGGAAGCAC20.13449899125756556No Hit
CTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTC20.13449899125756556No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG20.13449899125756556No Hit
CGTCACGCACCGCACGTTCGTGGGGAACCTGGCGCTAAACCATTCGTAGA20.13449899125756556No Hit
GGAGACGGCGAGCTCGTCGGTCCGCTCCGCCCGTCGCCCCACCAACTCCC20.13449899125756556No Hit
ATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGT20.13449899125756556No Hit
TGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTCTTCAGCTGA20.13449899125756556No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT20.13449899125756556No Hit
TCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT20.13449899125756556No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC20.13449899125756556No Hit
GCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGTATGTGCTTGG20.13449899125756556No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT20.13449899125756556Clontech Universal Primer Mix Long (96% over 26bp)
AACCATGCCACTGTTTCCATGGGCCCGAGTTACTTTTCCCCAGATGAAAC20.13449899125756556No Hit
CCCTACAGCCAGAAGAGATATGAGGAAATTGTTAAGGAAGTCAGCACTTA20.13449899125756556No Hit
CTCCTGCCCTGCCAACTTAGTAGAGGCCAGGGCTCTCACCTGTTCCCAGT20.13449899125756556No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA20.13449899125756556Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA20.13449899125756556No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT20.13449899125756556No Hit
GTACATGGGTGTCGACCGAGTCTACACTCTTTCCCTACACCTGTCTCTTA20.13449899125756556No Hit
GAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACC20.13449899125756556No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTAC20.13449899125756556No Hit
GTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGC20.13449899125756556No Hit
TTTCAGCCCTGATCAAGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGC20.13449899125756556No Hit
CTCCTCAGATTCATTGAACTAGGTCTGTCCCAATGTATGGGATGGCGGAT20.13449899125756556No Hit
GGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGC20.13449899125756556No Hit
CGTTCGTGCCGTGCCACTCGTCCACGCCGAAGTCGGCGTCGAAGCTGTCG20.13449899125756556No Hit
CTTACCGCATGCCGCCCAAGGCCATCATCAAGGGCCTGCTGGCGCCGATG20.13449899125756556No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG20.13449899125756556No Hit
ATATGACCTTGTTTAGAAAAAGGGTCTTTGCAGATGTAATCAAGTTATGA20.13449899125756556No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTAT20.13449899125756556Clontech Universal Primer Mix Long (95% over 21bp)
ATGTTAGCCAGGATGGTCTCGCTCTCCTGACTTCATGATCTGCCCGCCTC20.13449899125756556No Hit
GCAAACGCTCAGCATTCGTGACGATGGGAGAAAAGTCGGCGCCGGCGACC20.13449899125756556No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT20.13449899125756556No Hit
CATGGGTTGCAAACTGAAATATAAATAGGAGAGAGAGAGGCACTGTAAAT20.13449899125756556No Hit
GAGAAGTACACCTTTTTGCTAGCATCTCGCACGGCAAGAGCGATTGCCGA20.13449899125756556No Hit
GCCCCTCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGCGGTACCTGT20.13449899125756556No Hit
GAATGCAATGGAATGGAATCATCCGGAATGGAATGGAATGGAATGGAATG20.13449899125756556No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG20.13449899125756556No Hit
CACTGTTTTGAGCACTGTTATTAATTGTGATTTCCTTTTTCTGTTTTTCA20.13449899125756556No Hit
TTGCTCCACAGTCTGCTCCTTTCTGAATGCAAAATCCAATGAACCGTGGG20.13449899125756556No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.13449899125756556No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG20.13449899125756556No Hit
TAATAACTTTGAGCAGCATCATTCCTGGCCATTGGTCTATGCTCCAAAAT20.13449899125756556No Hit
GTGTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCG20.13449899125756556No Hit
GTTTCCCGTGTTGAGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGC20.13449899125756556No Hit
AAGGAGGTTAAAACCATTAGTTCCAGTAATGCCAGTTCCCAAACATGCAC20.13449899125756556No Hit
GCATGTTAATTTCCCTTAGAAGTTCCAAGCCCTGTTTCCTGCGTAAAGGT20.13449899125756556No Hit
GCCCAGGAGCCTTTGTGCGTGCACTGCCTGCCCATCTGTACATCCCATAC20.13449899125756556No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.13449899125756556No Hit
GAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATCTTTTCCCT20.13449899125756556No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)