FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003652800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003652800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1487
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA161.0759919300605245Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG120.8069939475453934Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG80.5379959650302623Clontech Universal Primer Mix Long (96% over 26bp)
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA50.3362474781439139No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT50.3362474781439139Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT50.3362474781439139Clontech Universal Primer Mix Long (96% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50.3362474781439139No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC50.3362474781439139Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC40.26899798251513113Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT40.26899798251513113Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.26899798251513113No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC30.20174848688634836No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT30.20174848688634836Clontech Universal Primer Mix Long (100% over 23bp)
CACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCAT30.20174848688634836No Hit
AATTGGGACTCATCAAATCCAACTTGCTGGGAAACTGATGGAGGACTTGA30.20174848688634836No Hit
AGCGTGGTGTGGTGCCCCCGACCTACAACTTGCGCCAATTATCGAGCTGG30.20174848688634836No Hit
GGTCGATGCGATGTCGCGGCTGACGGTCGCCAGGTCCGTCGCCGCGCCGA30.20174848688634836No Hit
AAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCG30.20174848688634836No Hit
ATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGGGGCGTGCGATCGGC30.20174848688634836No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC30.20174848688634836Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT30.20174848688634836No Hit
GGAGTGGGGGGAGGAGTTGCAGGCCGTGCTCCGCAGCAGCCAGGGGACAA30.20174848688634836No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT30.20174848688634836Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT30.20174848688634836Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG30.20174848688634836No Hit
CCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCT30.20174848688634836No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.20174848688634836No Hit
AAAATAATTATAGACAGTAGGTAAGTGACTGTGTGTGGCTATATTCCAAT30.20174848688634836No Hit
GCTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCG20.13449899125756556No Hit
TTCTGATATGGCGGCGGTCGGTTTCCAGTTTCGTTTCCCCGGGACCTCTT20.13449899125756556No Hit
TGCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTT20.13449899125756556No Hit
GGGAAAGACCCCTGGTTGGCCACGCGGCGGCGACTAACCTCAGACGACTC20.13449899125756556No Hit
GCCGCTGGTCTGCGCGAAGGCGGCCACGCCGTCCACGTTGGGAAACGCCG20.13449899125756556No Hit
CTATCAGAATGCCCTATACCTGTGAGATAGGCCAGGGACTTCTGTCCACA20.13449899125756556No Hit
CGGTCTGGACCAGGAGCGGGTTCGCCGGATCGCGTTCGATTTTCCTTCGC20.13449899125756556No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG20.13449899125756556No Hit
TTTTATTCTCTTGGTAATCTCTTCCAGTTAACTACTTCCGTTGTAGGTGA20.13449899125756556No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGT20.13449899125756556No Hit
ATCCTAGAGGACAAAATTAAACTCAATAGAGTGTAGTCTAGTTAAAAACT20.13449899125756556No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT20.13449899125756556No Hit
TTAAAAATCAACTTCATGAAGTCACAGATTTTTTAACAGTAAATAGTTTT20.13449899125756556No Hit
GGTGTTGAGGTTGCGGTCTGTTAGTAGTATAGTGATGCCAGCAGCTAGGA20.13449899125756556No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA20.13449899125756556Clontech Universal Primer Mix Long (95% over 21bp)
GTACCGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGAGGGGCCTGT20.13449899125756556No Hit
AAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACAGTAGG20.13449899125756556No Hit
GTACGAGAGGAACCGCAGGTTCAGACATTTGGTGTATGTGCTTGGCTGAG20.13449899125756556No Hit
CTTCCGATCTCTGGTACCGACAGAATCTGAGGCAAGAGCGTCGTGTAGGG20.13449899125756556No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT20.13449899125756556Clontech SMART CDS Primer II A (100% over 26bp)
GGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCT20.13449899125756556No Hit
TTTTTAGATTGATTGCTTTGTGACATTCACTTTTTAGAATATTTAGATAA20.13449899125756556No Hit
CACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCC20.13449899125756556No Hit
TCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATT20.13449899125756556No Hit
CATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACG20.13449899125756556No Hit
TTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATT20.13449899125756556No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA20.13449899125756556No Hit
ATACGAATGCCCCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAA20.13449899125756556No Hit
TTGGACACAGAATCCGAGTGATGCTGTACCCCTCAAGGATTTAAACTAAC20.13449899125756556No Hit
GAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTG20.13449899125756556No Hit
GGCTCAAGTCATCTACCTGCCTCAGCCTCCCAAAGTGCTAGGATTACAGG20.13449899125756556No Hit
CATCCCATGCGAGTCGATGGAAAATCAGCGCCATGGTCGAAGCGCGCACC20.13449899125756556No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACA20.13449899125756556No Hit
GAATTTTGGTAAATAGAACTAATCTTTGCACATACACTTCCCTCCACCAC20.13449899125756556No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG20.13449899125756556Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC20.13449899125756556No Hit
GGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTC20.13449899125756556No Hit
AGTTGTAAGAACGAACAGAAAGATCCAATTCTTCAATTGTCATTTCAAGA20.13449899125756556No Hit
GAACTGGGAACAGGTGAGAGCCCTGGCCTCTACTAAGTTGGCAGGGCAGG20.13449899125756556No Hit
GCCAGGCGTGTTCGGCTGGCCCGACCATGCCTGCCGCGGCTGCTCCATGG20.13449899125756556No Hit
GTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCC20.13449899125756556No Hit
GTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGATGCTTCCAATA20.13449899125756556No Hit
ATCCACAGCCCCGACCTTTGATGAGAACTCAGCTGTCCAGCTGCAAAGGA20.13449899125756556No Hit
CTCTACTCCGAGGCTGGAGTGCAGTGGCACAATCACAGCTCACTGCAACC20.13449899125756556No Hit
CCTTTGTGGGAATCACAAAAAGGCACCCCATGGGTGCCAGTGAGTTGCCC20.13449899125756556No Hit
GCAAGGCGTCGAGCCGCTTCTGCCCGGCCTCGGCACTGCCGAAGGTCTCG20.13449899125756556No Hit
ATCTACAAAGGAGTTCTTCACCAAGCACAAGTACTTTGGTTTAAAAAAAA20.13449899125756556No Hit
GCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTC20.13449899125756556No Hit
AGTCTACACCTTTCCCTACACGACGCTCTTCCGATCTCACTGTCTACTGG20.13449899125756556Illumina Single End Sequencing Primer (96% over 29bp)
CTTGTTGCCCAGGCTGGAGTGCAATGGCATGATCTCAGCTCACTGCAATC20.13449899125756556No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCATGGTATCAA20.13449899125756556Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT20.13449899125756556No Hit
TGGTGACGATGCGCGTGCGCACCGAGTTCCTCGACTCCGGGGAGGTGTCC20.13449899125756556No Hit
ATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGG20.13449899125756556No Hit
CACCAAATCGCACTGGCTCCTGGACTCTTTTCCTATCTTCACCACGAACT20.13449899125756556No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA20.13449899125756556No Hit
TTTTCCCCCTTGACTTACATTAAAAAAAGAAAGACTAAAGTCTCTAAGTA20.13449899125756556No Hit
GTCGCCATGTTCCCCCAAAATCCATCCATGGCAGCTGCTGGGATGAATGC20.13449899125756556No Hit
CGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGG20.13449899125756556No Hit
GCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAGTACCTGTCTCTTA20.13449899125756556No Hit
ATTCCATTCCATTCCATTCCACTCCGTTCCGTTCCGTTCCGTTCCGTTCC20.13449899125756556No Hit
AGATCGTGTTCGACGACGTGCGCTTCGGCTATGGCCGTGAGGTTATTGAC20.13449899125756556No Hit
GACTACAGAGGTTGAGGCTGGAGAATCACTTGAGCCTGGAAGTTTGAGGT20.13449899125756556No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC20.13449899125756556Clontech Universal Primer Mix Long (96% over 26bp)
GGGCTATATTTTCTCTCCTCGAGTTGCTCCTCATGGCTGGGCTCATTTCG20.13449899125756556No Hit
GTTTCAGGGAATTATTCTTTTTTCTGCAATAGTTGGATTTATCTACGGGT20.13449899125756556No Hit
GAACCTGCGGCTCGGCCAAGCCTGTGGCCCAGCCTGTCGCTGCGCTGGAG20.13449899125756556No Hit
GTTCACCCTGAGTGCTCTTGCCTCAGTATGGCAACTGATTATGAGTTCAG20.13449899125756556No Hit
ATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAG20.13449899125756556No Hit
ATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGT20.13449899125756556No Hit
AGTTTAAAGTGATTTTATTCATCAGTGTCTCTGTTCAGGACAAAAAAAGA20.13449899125756556No Hit
GTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGAGGGTG20.13449899125756556No Hit
GAGTTAGCACCCACCATTGGATGAAGCCCAGGATAAGTGACCCCCCCGGA20.13449899125756556No Hit
GTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATCTGCTCGGGCGT20.13449899125756556No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG20.13449899125756556Clontech Universal Primer Mix Long (96% over 26bp)
GAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTC20.13449899125756556No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC20.13449899125756556Clontech Universal Primer Mix Long (96% over 26bp)
AATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTT20.13449899125756556No Hit
GTATCAACGCAGAGTACATGGGATGACGGCGACACGAATCTACACTTCTG20.13449899125756556No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACCTGTCTCTTATACAC20.13449899125756556No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTAT20.13449899125756556Clontech Universal Primer Mix Long (95% over 21bp)
GCATGTGAATATATCATTGGGGGAAAATTCCATGGCTTGTTAGAGCAAGT20.13449899125756556No Hit
CCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAAC20.13449899125756556No Hit
GCTCATATAAACTCTGCTAAATTTAATTTGTCTAAAGTTTTTCTTTTAAC20.13449899125756556No Hit
CTTCCGATCTGGTACACAGGTCCTGAAAAACGAGAGCGTCGTGTAGGGAA20.13449899125756556No Hit
CCAATAAACATGATCTTTCTGCTCCGCTGAGAAGTACACTTTTTTGCTAG20.13449899125756556No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT20.13449899125756556No Hit
GGGTTTCGTACGTAGCAGAGCAGCTCCCTCGCTGCGATCTATTGAAAGTC20.13449899125756556No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT20.13449899125756556Clontech Universal Primer Mix Long (96% over 26bp)
GAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCC20.13449899125756556No Hit
TCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCT20.13449899125756556No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA20.13449899125756556Clontech Universal Primer Mix Long (96% over 26bp)
GTGTAGGGAAAGAGTGTAGACTCGGTCGACACCCATGTACCTGTCTCTTA20.13449899125756556No Hit
GATCTATCCCTTTTTTGGCCTGTTGGTACCAAAAAAGGGATAGATCGGAA20.13449899125756556No Hit
TTCCTACACATCGGGCGAGGCCTATATTACGGATCATTTCTCTACTCAGA20.13449899125756556No Hit
TCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCC20.13449899125756556No Hit
CTGAGACACAGCTGGTACTGAGTGTGTGGTAGTAAAGTTCTGGGCCATGG20.13449899125756556No Hit
AATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACT20.13449899125756556No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA20.13449899125756556Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGCATCGCCAGAAGTGCCACGACGAGTTGAAGGCGATGCTGGCCGACCT20.13449899125756556No Hit
GAGGTAGAAGGATTAGAGACAAACCTTTGGGACCATGTAACGTATAACCG20.13449899125756556No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA20.13449899125756556Clontech SMART CDS Primer II A (96% over 26bp)
GGACGGTGTCTTTTTCTGGACAGCTGAGTTCTCATCAAAGGTCGGGGCTG20.13449899125756556No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTC20.13449899125756556No Hit
AATTGAATGATTACGCAGCTCAGAGTCAATCTGCAAAGACTGGGAGAGTT20.13449899125756556No Hit
GGTTAGGCCACTCGGGAAACTGAAGCACAGAGAGTTTAAGTAACTTCCTG20.13449899125756556No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)