FastQCFastQC Report
Tue 10 May 2022
EGAF00003652895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003652895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1863536
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAG58910.31611946321401896No Hit
GTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCCAAGCCTGTGG45240.2427642932575491No Hit
GTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCGGGTGGGCAGCGTCTTGT42640.22881232238067847No Hit
CTCCTGGGGCTCTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTT31690.17005306041847326No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA31270.16779928050759416No Hit
GCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCC30380.16302341355358846No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT29460.15808656232023421No Hit
GGGATGGACGGTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCGGGTGGGC29230.15685234951189567No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG29080.15604742811515313No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC28620.153579002498476No Hit
GCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCCAAGCC28550.1532033725133295No Hit
CCCCGAGCCCCCGCGCCATGAAGCTCGCCGCCCTCCTGGGGCTCTGCGTG28230.15148620686694542No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA27990.15019833263215737No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA27690.1485884898386723No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT26920.14445656000206059No Hit
GTACATGGGAGCCGCAGGTTCCCCGCGCGCCCCGAGCCCCCGCGCCATGA26170.1404319530183479No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG25240.1354414403585442No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT25120.13479750324115017No Hit
CCTGGGGCTCTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAG24310.13045092769874045No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA24310.13045092769874045No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT23400.12556773789183573No Hit
CCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAG23010.12347494226030513No Hit
GGTCTATAGAGCTCACACCCATACTCCCAGCTGGGACCCAGCCTTCCCTG22560.12106017807007752No Hit
GTCTTGTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGT22340.11987962668818847No Hit
CTCCCACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG22020.1181624610418044No Hit
CTTCTGGGAGCCCTCTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCA21830.11714289393926385No Hit
GCCAAACACTGTCAGGGCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCC21630.1160696654102738No Hit
GAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGG21580.11580135827802629No Hit
TTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGG21500.11537206686643027No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA21270.11413785405809172No Hit
CTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCAGCAGGAGCTTCAGC20940.11236702698525812No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA19930.10694722291385839No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC19730.10587399438486834No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG19670.10555202582617132No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA19650.10544470297327233No Hit
ACCAGAGACAGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTC19620.1052837186939238No Hit
GGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCC19220.10313726163594371No Hit
GTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGTCAGGG19220.10313726163594371No Hit
TTCTTAGTGGGCTCGGCCAAGCCTGTGGCCCAGCCTGTCGCTGCGCTGGA19080.10238600166565068No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)