FastQCFastQC Report
Tue 10 May 2022
EGAF00003653267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003653267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385144
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC19190.4982551980557921No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT17220.44710549820326945No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG15030.3902436491286376No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC14440.37492470348752677No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA13420.3484411025486571No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA11510.29884926157489144No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA11390.2957335438173774No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT10660.2767795941258335No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC10530.2734042332218599No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA10000.25964314645950604No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA9710.25211349521218035No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT9680.25133456577280183No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT8930.23186132978833893No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT8810.22874561203082483No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC8620.2238123922480942No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG8460.2196581019047421No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT7920.20563737199592882No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT7480.19421307355171052No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG7390.19187628523357497No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG7320.19005878320835842No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA7310.18979914006189894No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT7300.18953949691543942No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC7160.18590449286500632No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG6830.17733626903184266No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT6640.172403049249112No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG6550.17006626093097646No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC6400.16617161373408387No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA6240.16201732339073177No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT6230.16175768024427226No Hit
GAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCG6110.15864196248675821No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT6070.15760338990092018No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG5830.15137195438589204No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC5720.14851587977483746No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT5570.14462123257794487No Hit
GTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCC5460.1417651579668903No Hit
CTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCT5440.1412458716739713No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT5360.13916872650229525No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT5320.13813015391645722No Hit
TTCCAGAGCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGA5280.13709158133061922No Hit
GCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGC5260.1365722950377002No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC5160.13397586357310512No Hit
ACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGA5050.13111978896205057No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA5050.13111978896205057No Hit
GAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGG4990.12956193008329353No Hit
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCA4930.12800407120453647No Hit
GAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGG4800.1246287103005629No Hit
GTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAG4770.12384978086118438No Hit
TCCCTATACGCGATGGATCGAGGTCATGACTGGACACTGCATCGGAAGAC4690.12177263568950836No Hit
TTTATGAACTAGCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTT4680.12151299254304884No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG4650.12073406310367032No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC4620.11995513366429181No Hit
TTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGGTCAGGGCCGCT4610.11969549051783229No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG4530.11761834534615626No Hit
GAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCA4470.11606048646739921No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4410.11450262758864217No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG4300.1116465529775876No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG4230.10982905095237105No Hit
GTACATGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCA4220.10956940780591155No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA4200.10905012151299254No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC4190.10879047836653304No Hit
GGCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATA4150.107751905780695No Hit
TCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCA4140.1074922626342355No Hit
GCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTC4050.10515547431609995No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC4020.10437654487672142No Hit
ATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGC3960.10281868599796441No Hit
ATTCAAATGAGCATCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGA3960.10281868599796441No Hit
GTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGT3960.10281868599796441No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA3950.1025590428515049No Hit
ATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTG3940.1022993997050454No Hit
TCATGCGACTGTTCCCCGGGGTCAGGGCCGCTGGTATTTGCTGTAAAGAG3930.1020397565585859No Hit
GTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAG3910.10152047026566688No Hit
GGACAAGGGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGG3880.10074154082628836No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)