FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003654739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003654739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9
Sequences flagged as poor quality0
Sequence length100
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGAGCAGCTTCGTGCCAGTTGAGGCCAGCCAATCTGGCTGCGATCATG555.55555555555556No Hit
GCTCAAGAATCCATTCTGGCGCGGCAAGCGCTTCCAGGCTGTTATCCAAC222.22222222222222No Hit
GTTTGCTGGCTGGCAAACTGATGTAGCGGATCGCGCTGACGCTGATACTC111.11111111111111No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT111.11111111111111Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)