FastQCFastQC Report
Tue 10 May 2022
EGAF00003654897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003654897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34494
Sequences flagged as poor quality0
Sequence length100
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC7222.0931176436481707No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT2870.8320287586246884No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG1600.46384878529599355No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC1470.426161071490694No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG1360.3942714675015945No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA1360.3942714675015945No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC1350.39137241259349453No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG1350.39137241259349453No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC1330.3855743027772946No Hit
GGCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATA1260.3652809184205949No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG1180.3420884791557952No Hit
GCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGC1100.3188960398909955No Hit
GGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGC1040.30150171044239577No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT940.2725111613613962No Hit
GTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTTGGATTCTGAGCCC940.2725111613613962No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA930.26961210645329625No Hit
CACTAGAAGTCGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGTGGT900.2609149417289963No Hit
CTGCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGA820.23772250246419666No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG800.23192439264799677No Hit
TTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGGTCAGGGCCGCT780.22612628283179684No Hit
TTCCAGAGCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGA780.22612628283179684No Hit
CAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGGCACG770.22322722792369687No Hit
GTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAAGTGTCTTCCGATGC730.21163100829129705No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG730.21163100829129705No Hit
GTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGG730.21163100829129705No Hit
CAGCTAAACCGACGCAGTTTTGCTCCTTGGATTCTGAGCCCGGGCATCGC730.21163100829129705No Hit
CCGATGCAGTGTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCC710.20583289847509711No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA690.20003478865889718Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT690.20003478865889718Clontech Universal Primer Mix Long (96% over 26bp)
TCATGCGACTGTTCCCCGGGGTCAGGGCCGCTGGTATTTGCTGTAAAGAG690.20003478865889718No Hit
GTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGGT680.19713573375079724No Hit
ATCGCAGACGGTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGC670.1942366788426973No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA660.19133762393459733No Hit
GTTGTAGGTCGGGGGCACCACACCACGCTCGCTTGGCAGTAACGCGAGCA640.1855395141183974No Hit
CGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATAAGATTC640.1855395141183974No Hit
TATTGAGGCTGCTTCGTGTCGGCACGAAGTGTCTTCCGATGCAGTGTCCA630.18264045921029745No Hit
CTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGATAATTGCG620.17974140430219748No Hit
GTGTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCG620.17974140430219748No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT610.17684234939409751No Hit
CGGTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTC610.17684234939409751No Hit
GGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATAAGATTCCATGC610.17684234939409751No Hit
GTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGCCTT610.17684234939409751No Hit
TCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGGTCAGGGCCGCTGG600.17394329448599755No Hit
GCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTG580.16814518466979766No Hit
CCCCTCGTTTATGAACTAGCCTATCGCAGACGGTATCAACAGGAACATCC570.1652461297616977No Hit
ATCTCAGCTAAACCGACGCAGTTTTGCTCCTTGGATTCTGAGCCCGGGCA560.16234707485359773No Hit
CTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTG560.16234707485359773No Hit
GAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCG560.16234707485359773No Hit
CGTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGTGTGG560.16234707485359773No Hit
CAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCG550.15944801994549776No Hit
TCACAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTA550.15944801994549776No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT550.15944801994549776No Hit
GCGTACGGCGGGACCTCTGAGAATTGGGATACAGGACCCAAAAGGCTGAA540.15654896503739782No Hit
GCCGTACGCTGAGGACCACCTGAAACGGGCATCGTCGCTCTTCGTTGTTC540.15654896503739782No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT540.15654896503739782No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC530.15364991012929785No Hit
GTTTTGCTCCTTGGATTCTGAGCCCGGGCATCGCCCCTCGTTTATGAACT530.15364991012929785No Hit
GTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCGTTACT530.15364991012929785No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA520.15075085522119788No Hit
ATATTGAGGCTGCTTCGTGTCGGCACGAAGTGTCTTCCGATGCAGTGTCC520.15075085522119788No Hit
GTAGATCACAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTC510.14785180031309791No Hit
TCCCTATACGCGATGGATCGAGGTCATGACTGGACACTGCATCGGAAGAC510.14785180031309791No Hit
GGTCAGGGCCGCTGGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGA500.14495274540499797No Hit
CAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTATAA500.14495274540499797No Hit
GTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATAAGATTCCATGCC490.14205369049689803No Hit
TCCCAATTCTCAGAGGTCCCGCCGTACGCTGAGGACCACCTGAAACGGGC490.14205369049689803No Hit
GTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTC490.14205369049689803No Hit
AGCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTTAGATATTGAG470.1362555806806981No Hit
TTCTGAGCCCGGGCATCGCCCCTCGTTTATGAACTAGCCTATCGCAGACG470.1362555806806981No Hit
CATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGCAT470.1362555806806981No Hit
ATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGAC460.13335652577259813No Hit
GTCGCAGCCCGGGTCGATTATAACAACGGTGCAATCTCAGCTAAACCGAC460.13335652577259813No Hit
GTGTCTTCCGATGCAGTGTCCAGTCATGACCTCGATCCATCGCGTATAGG460.13335652577259813No Hit
CCACACTAGAAGTCGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGT460.13335652577259813No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT450.13045747086449816No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT450.13045747086449816No Hit
GTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTAT430.12465936104829825No Hit
GTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGTGTGGT430.12465936104829825No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA430.12465936104829825No Hit
AGCATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGC420.1217603061401983No Hit
GCTTCGTGTCGGCACGAAGTGTCTTCCGATGCAGTGTCCAGTCATGACCT420.1217603061401983No Hit
CGTTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGGTCAGGGCCG420.1217603061401983No Hit
CACTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCAGCGTG410.11886125123209833No Hit
TCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTGGC410.11886125123209833No Hit
GCATAAGATTCCATGCCTAGATGTGATACACGTTTCTGGAAACTGCCTCG400.11596219632399839No Hit
GTATACGGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACG400.11596219632399839No Hit
GATTATAACAACGGTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTT400.11596219632399839No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT400.11596219632399839No Hit
GTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAGTATGCTGCAAACA400.11596219632399839No Hit
GAGCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGAAATAT390.11306314141589842No Hit
TTTATGAACTAGCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTT380.11016408650779846No Hit
CCCTATACGCGATGGATCGAGGTCATGACTGGACACTGCATCGGAAGACA380.11016408650779846No Hit
ATATAGGCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTG380.11016408650779846No Hit
GGCATAAGATTCCATGCCTAGATGTGATACACGTTTCTGGAAACTGCCTC380.11016408650779846No Hit
GCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTTAGATATTGAGG380.11016408650779846No Hit
GTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGGAAGGAGACATA370.1072650315996985No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA370.1072650315996985No Hit
CCTAAAGTATGCTGCAAACATATGCAGATCCCCTGAGACAGAGTCGCTAT370.1072650315996985No Hit
GAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAAGTGT370.1072650315996985No Hit
CATATGCAGATCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCG360.10436597669159853No Hit
GGGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCC360.10436597669159853No Hit
CTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCT360.10436597669159853No Hit
GAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGG350.10146692178349859No Hit
TCCCCGGGGTCAGGGCCGCTGGTATTTGCTGTAAAGAGGGGCGTTGAGTC350.10146692178349859No Hit
GTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGATACAGGACCCAAAA350.10146692178349859No Hit
CGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGG350.10146692178349859No Hit
GATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGA350.10146692178349859No Hit
CTTTCAGCCTTTTGGGTCCTGTATCCCAATTCTCAGAGGTCCCGCCGTAC350.10146692178349859No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)