FastQCFastQC Report
Tue 10 May 2022
EGAF00003655094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003655094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634400
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT12070.19025851197982346No Hit
GTACATGGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAAT8900.14029003783102145No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC8580.13524590163934427No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA7650.12058638083228246No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC7520.11853720050441362No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC7370.11617276166456494No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT7270.11459646910466582No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC7020.11065573770491803No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT6990.11018284993694831No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT6960.10970996216897856No Hit
CCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACACAAACTACC6670.10513871374527113No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA6630.10450819672131147No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT6610.10419293820933165No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG6560.1034047919293821No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA6370.10040983606557376No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)