FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003655101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003655101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCCCTACAAGCTAAAGGAGTGGAAGCGAGGGCAGTACGTGGAACTCAC42.9850746268656714No Hit
CTATGAATGGGTGGAACTCTCTGAGATATCCTACATTTCCCGTTAGAACA32.2388059701492535No Hit
CTACTCTGGTGGCTCTAGCAGGTCTGGCTAAAACAAAAGGATTCCATACA32.2388059701492535No Hit
ATGCTGGGGAAAGGGAAGAGAGTGGCTCAGCCTGCAGGTAAATAGGCTAG21.4925373134328357No Hit
TCAGAGCATGTAGCAAATTTATTATCCGTGGGTGAGAAACTGGCCTCTCT21.4925373134328357No Hit
TAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACG21.4925373134328357No Hit
GCCTGTTCCTCATGCGTGCGAGCCGTGGATGGGAAGGCGGTCTGCGGTCA21.4925373134328357No Hit
GCCAGCCCGTACGTGGAATTGTTGGCTCTCCCAACAACCTCCTCTATGGT21.4925373134328357No Hit
CTGCAGAGCACCCGCTTCTCCCTGGCCTTCATCCCGAGTTGCACTAACCA21.4925373134328357No Hit
TTATTTCTATCTACCAGAAAATAAAGTCGTATCTTTTCTTAGTATAATAT21.4925373134328357No Hit
GTCAGGTGGTCACCCATCTTCTTGATAAGCTTCACTTCCTCATCTAGGAA21.4925373134328357No Hit
GAGCGAGAGGGCTCCTTCCACAGCAATGACCTCTTCTTGGATGCCCAGCT21.4925373134328357No Hit
TCCTAATACTGTCATCCTCAAAGAAAGTGTATTGGTTTTTTAAAAAAGAA21.4925373134328357No Hit
TTACCAAGTACTGCAAGAAATGGCAGGATGAGGATGGCAAGAAGCAGCTG21.4925373134328357No Hit
TTGTTGATGAAGCCATCAATGAGGACAACAGTGTGGTGTCCTTGTCCCAG21.4925373134328357No Hit
GTATTGGCGGCGCAGATGGTAAACATGGCCTGCATGAATATCTGCAGACC21.4925373134328357No Hit
GCCCTGCGGCGTCTCGAATACCGCAACCACTTGCGGCATGGCCCGTGCCG21.4925373134328357No Hit
CGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATTGTGTAGGCGAATAGG21.4925373134328357No Hit
GTACAAGAGATAGAAAGACCAGTCCTTGCTGAAAGACAAGTCTGAATGCT21.4925373134328357No Hit
ACATGGTGAAACCCCGTCTCTACTAAAAATGCAAAATTAGCTGGGTGTGG21.4925373134328357No Hit
GAATTGTTTTTACTTTAACAATGATTGGAATTAAGCCAAAGGTAGTTAAA21.4925373134328357No Hit
ATAGTCTACTTACTCTTTCTACAACATGCCATGCTGGTTCCAAACTGAGA21.4925373134328357No Hit
GCTCAAGGAGGAGCAGGAAGTGGCTATGCTGGGGGCGCCCCACAACCCTG10.7462686567164178No Hit
GTACATGGGGGGTCTGGTACATCTTGCCGGTGTAGCCGCGTTCCTTCAGC10.7462686567164178No Hit
GGGGGAAGTAGTCGTCAAGCACTTGGTTGCACTGGGGCATGAGCAGCTTC10.7462686567164178No Hit
AAGGTCTTAAAGTTTATGTAGAGCCTCCAGCAAATCAGGGAGGAGAGAGG10.7462686567164178No Hit
TGAATAAATTCTTTTAAGAATGGATAAATACAGTTGAGGAGTTCAATATT10.7462686567164178No Hit
CCTTGAAGAAGTATGACGGTCGAACCAAACCCATTCCAAAGAAAGGTACT10.7462686567164178No Hit
GCACCCGGACCTGATTTAGATAATGAGATTCTAAACCTTGAGCTGGTACT10.7462686567164178No Hit
CACTTATGCCTACGGGGTCATGGACAGTGGCTATCGGCCTAATCTTAGCC10.7462686567164178No Hit
GCTCCGATAATAGCCAGTGGCACTGGAGCAAGTACTGGGTTAAGGCTAGA10.7462686567164178No Hit
CAACCTGCACGAGCTGTGCGTGCTGCGGCGTTACCGGCTGCAGCGGCGGG10.7462686567164178No Hit
CTTCACGCCAATGGCGCCTCAATATTCTTTATCTGCCTCTTCCTACACAT10.7462686567164178No Hit
CGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAG10.7462686567164178No Hit
AGATTTTGCATGAATTATTTTTTAAACAAAACTATATGAAAAATATGTAC10.7462686567164178No Hit
GAACTAATGTGTTCTTCAAAGAAAGCAGACAGGCGCAAACTCATGCTGTA10.7462686567164178No Hit
GAGGGGGGGATGGAGTCTCACTCTTTCACTAGGCTGGAGTGCAGTGGTGT10.7462686567164178No Hit
CCCTGGAGACGGAGGTTGCAGTGAGCCGAGATTGCGCCACTGCACTCCAG10.7462686567164178No Hit
TTTCGGTGGAGCGTCAGTGGGCTTTTGCTCCAAGGAGTGCCTGCTCATTT10.7462686567164178No Hit
TCTATGAACCATGTGAATGGATTACTTATCCTCTCCATGCAAGAAAACAA10.7462686567164178No Hit
GATTCAAGGAGGTGACATCACCACTGGAGATGGCACTGGGGGTGTGAGCA10.7462686567164178No Hit
GGCCAAATAAGCGCCGGCTATGCCCCTGTATTGGATTGCCACACGGCTCA10.7462686567164178No Hit
ACATGGAGGAATGAAGTCCTAATTACTATACATGCATATTTTTTTGACAG10.7462686567164178No Hit
CCCTGAGCTCCTCCAGGCTGAAGCCGCGGCCGGCGCGCACCTTCGTGTGG10.7462686567164178No Hit
GCATTTTCACAAATATCTTCTTTAATGCTCCCAAGAACATTAAATAGTGT10.7462686567164178No Hit
CAGCCAGTTACTTCATGCTTTTCTGGGCCACCCCGGGTCTGAATGGCCAG10.7462686567164178No Hit
ATTACTGTACCTCTTTTCTTCATAAATTACCCAGTTTTAGGTATCTTTAT10.7462686567164178No Hit
ACACACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGA10.7462686567164178No Hit
ATGTCTGGGCCGAGAGCTACCTGATCGCCGCGGCCTTCACGACGCTCACG10.7462686567164178No Hit
ATAGAAGTACAGTTGTTACATATTTAATGAATACAATTTGATGGGTCTGA10.7462686567164178No Hit
TCTCCACACTAGAAGACGACGAACAACGAAGAGCGACGATGCCCGTTTCA10.7462686567164178No Hit
GGGCACCAGCTCCTTGAGGCGTGAGTAACAGCCGTTCATGTCGTAGAGCA10.7462686567164178No Hit
CCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGC10.7462686567164178No Hit
GGGTGGCCCGGTGGAAATCCTGCCCTTTCTGTACCTGGGCAGTGCGTATC10.7462686567164178No Hit
GCTTTTTGATGGGTTTGTTTGTTTGTTTCTTGAAAATTTGTTTAAGTTCT10.7462686567164178No Hit
GACCATGGCCGGATCCAGCCCTAACACGCCTCAGCCCCTGTCTCTTATAC10.7462686567164178No Hit
GTATTATAAATCCTGGTCTTCCATCTGGTGTTTTTGATGGAGGGACATAT10.7462686567164178No Hit
TTCTTGGCCTCCTTCTGGGCCAGAGCGGAGTTGAACGTCACCTTCACCAT10.7462686567164178No Hit
CCTTGACAGGTAGTAACTTTTCACCCCACATCACTGAACGCTTAACACTC10.7462686567164178No Hit
ATTTTATTTATGTCCCATGTGTTTCTTCTTCCTTTGTTTCTCCTTTACTG10.7462686567164178No Hit
GATAAAAGGAACCACAAAAATCCTTAGTAACTGGGTAAGAGAATATGAAG10.7462686567164178No Hit
CCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACA10.7462686567164178No Hit
GTGTGTGTCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT10.7462686567164178No Hit
ACAGTAAGGTGTAATTACAGAATTTACGAAATGTAAATGGCCTCTACAGA10.7462686567164178No Hit
GAAGTCAGCAGCAACCATGTCTTGATTTACCTTGATAAGGTGTCAAATCA10.7462686567164178No Hit
ATTTTATTTTGATTCTGCAAGCTCAAGTTGCTGTCAAAATATAAAATGAT10.7462686567164178No Hit
GTCGGGCGCCCTGTTCTGAAGGCAAAGGGTCCCCTGCCTCGCACGACTCG10.7462686567164178No Hit
GAGCTAGTAGAACCTCGCAATGACAGCCGCAATGGCAGACCCAATGGAGC10.7462686567164178No Hit
CCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCACG10.7462686567164178No Hit
GGTGGGCGATGCGGCGCACCGTATCCATCCCATTGCCGGTCAGGGCCTCA10.7462686567164178No Hit
CACTGCTGTTTCCCGTGGGGGTGTGGCTAGGCTAAGCGTTTTGAGCTGCA10.7462686567164178No Hit
GGTGAGGGGCAGCTGTGGTCACAGCTACTCGGCAGGCTGAGGTGGGAGGA10.7462686567164178No Hit
GGATGCCTTCGACGTGATCATCACTGACTCCTCAGACCCCATGGGCCCCG10.7462686567164178No Hit
GTATCTGATCGTCTTCGAACCTCCGACTGTCGTTCTTGATTAATGAAAAC10.7462686567164178No Hit
GTGTTCTTGTCGTAGCTTGCTTCGCTGCTGCCGGTGTAGAACAGGCCTTT10.7462686567164178No Hit
ATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCACCACCTCTGCCCAC10.7462686567164178No Hit
CTACAGAGTAGGGACACCCCCACTTCCATTTCAATTCTGAAGCAAGGAAG10.7462686567164178No Hit
TGAAAGGAGAGTCTCGGCATCCACAAAAAGTGTAGGACATTCAGAGCAGG10.7462686567164178No Hit
CCTCGGTTTTGCCGATGGCGATCCTTAAGAAATTGTGGTTTGTCGCCCTC10.7462686567164178No Hit
TTATAATGTCAGTCAAAATTGACCCAGAGGAAAGAAAACATTTTTGGAGA10.7462686567164178No Hit
GGGTATGGGGCCAGGCCCCTGGATGAATATCCTGTGGGCCTGGTTTATTT10.7462686567164178No Hit
CCTCTGTAGTAGGTTTCTTCTCTGCAGGCTTCTTTTCAGGGGCTGGTTTC10.7462686567164178No Hit
ACAAAAATTAGCCTGGCATCGTGGTGGGTGCCTGTAATCCAAGCTACTCA10.7462686567164178No Hit
ATCATATGTTCTAAAAGAGAAGGAAGAGAAAATCCTACTTTCTCAAAATG10.7462686567164178No Hit
GAAGAAGAAAAGATATAAGGTGAAGTAAGGACAACAGTGCAATAAAAAAA10.7462686567164178No Hit
GAGGTGCCTGAAACACTTATTGTTGGTGCTTAATTATGTTTGAATAATTT10.7462686567164178No Hit
ATCTGGAACAAGGTAAAAAGGGGTCACTATGGAGTTCAAAGGACAGAACT10.7462686567164178No Hit
GTCCAGGGCCTGTATTCAGTCAGAATCACTGCTGGAAGAGGAGGAGGAAG10.7462686567164178No Hit
GGAATCCCACGCGCAGCAGGCGCCCCTCGTCGGCGATGAGTTCCGCATGC10.7462686567164178No Hit
GTGTAGAAGGGATAGATGTAGTTTGCTGGGGAGCAGGTGTCGTAGGCTGC10.7462686567164178No Hit
CAATGTATCCTCCAACTAGGACCCTAATGTCAAAAATGAGCTAAGGAGGA10.7462686567164178No Hit
CCACGGAATCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTCCGG10.7462686567164178No Hit
GACTGGGAGAGCGGGCTGAATGCAATGGAGTGTGCATTACATTTGGAAAA10.7462686567164178No Hit
CCACGGGAGGCAGCCCAGAGGCCACCGGCACAGGGTGGTGGCCCCCAGAT10.7462686567164178No Hit
GCATGTTGTTGAGCGCCGCATCGAAACGCCCGGCCACAATGGACAGGCGA10.7462686567164178No Hit
GTGTGAGAACCGGCCTCGCTCTGGTTGTAGTAGCCGCGCAGGGTCCCCAG10.7462686567164178No Hit
GATCTACCTTCAAGTGTTTTGGCTCATTACTCGGTGGTATCAACGCAGAG10.7462686567164178No Hit
CGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCC10.7462686567164178No Hit
ATATTTGTTCTTTGTTCTTTTTCTAACTGATTTTGAATTATTTTTAATGA10.7462686567164178No Hit
CTCCATTCACCCCTGCCCTCTGCTCCTAGCAATCATCACTCTACTTTCTA10.7462686567164178No Hit
CTATTTAGGACGCTCATGGTAGCTTGGATCCGAGCAAACCTCTGTGTGTA10.7462686567164178No Hit
CCATCAACCTCAATGCCATTAACAAGTGCCCCCTGCTGAAGCCCTGGGCC10.7462686567164178No Hit
CGGCGAGACACAAAGCTCTGACAGTTGACGGCTTCTCGCGCCTTCAGAGG10.7462686567164178No Hit
ATCATATGTTCTAAAAGAGAAGGAAGAGAAAATACTACTTTCTCAAAATG10.7462686567164178No Hit
GGCACCGATTCCTTCTTCCAGCCGCGGCGCGCAGCCTTCTGGGTGCTGGC10.7462686567164178No Hit
GAAGAAATTCAAAAGAAAAGAACCCGCCGAGCAGTCAAATTCCAGAGGGC10.7462686567164178No Hit
GATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC10.7462686567164178No Hit
ACCGAGAACTGCGCGGTCCTGTCAGGCGCGGCCAACGGCAAGTGGTTCGA10.7462686567164178No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)