FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003655102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003655102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGCAGAAGCAGGGCGACCATCGACATCACCGGCGTGGTGTCGACCAG42.9850746268656714No Hit
GTTTGCATTTTGGGATCATAAAGATGCCTCCTAGTCCCGTAAGCTGTCAT32.2388059701492535No Hit
GTTTTAAGGCTTGTTTTGAAATAACATGTGGACAGCATCATGTGGTCACT32.2388059701492535No Hit
TCTCTCATCTTAAAAAAAAAAAAAGCCTGCTCAAACCTGCCCCCACCCAA21.4925373134328357No Hit
CTAGAAAACTGACTTTGATTTTTCTCTTAGGTTGTTTTGTTTTGTTTTGT21.4925373134328357No Hit
GTCGTGCGCGAGGTGCTGGTCGCGGCGGCCGCGCGGCTCTGGGGCGTGGC21.4925373134328357No Hit
GTTTCTGGCCTGCTGAAGATCATTGGATTTTCCACATCAGTAACTGCCCT21.4925373134328357No Hit
CCCAGGAAGTGGCCAAAAGCTAGACAGATCCCCGTTCCTGACATCACAGC21.4925373134328357No Hit
ATCTCATCCTGCCCAAACACTTGGTTCACAGGTACCTGCTGCTCAAGCCT21.4925373134328357No Hit
CAATAGATTAGTTAATGCTATGAGAAGACTAAGCCATCTTATTCCTTTCC21.4925373134328357No Hit
CGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACG21.4925373134328357No Hit
CATAATGACATGTGGATGTTGACAGCAGACCACGGAGGATATGTGAAATA21.4925373134328357No Hit
GTCATCAATGGGCAGCACATGGATACGTTTGCCGTACTTCACATCAGGGC21.4925373134328357No Hit
GCTCTGGAAGGCGTGAGCCACTTCTTCCGCGAACTGGCCGAGGAGAAGCG21.4925373134328357No Hit
ATCCAGCGTACTCCAAAGATTCAGGTTTACTCACGTCATCCAGCAGAGAA21.4925373134328357No Hit
TGCTGGGGGAGAAGGAGGCTCGGGACAAAGTGGGAGAAGTGCTGGGAAGG21.4925373134328357No Hit
GAAAAATAATGAAAAAAAGTATGCTGCACAACAATAGAAAAACAAAAATC21.4925373134328357No Hit
GTACATGGGAAGTCTCGGGTGGTCGGGAACCCCTTTGATAGCAAGACCGA21.4925373134328357No Hit
TATTCGCACCCTCGAAAAATTCGCTAAACTCTGACGCGCACAGGCGGAGG21.4925373134328357No Hit
AGGCACCCCTCCCGTCGACCCCACAGTTCAGTGTGAACACCGAGCGCTCG21.4925373134328357No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA21.4925373134328357No Hit
GGTGAGACAGGTTATGCCATCTTCCAAAGTGTCTAATTGCCTCAGGCGTG21.4925373134328357No Hit
GATTTCAATACAGCCCAAACCAAAAGGCTTACAGTCAACAGAGGGTTAGC21.4925373134328357No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTGGGTTTATGTTAAACCCTGTCGTT10.7462686567164178No Hit
GGCATGGAGGCCTCTGCTCTCACCTCATCAGCAGTGACCAGTGTGGCCAA10.7462686567164178No Hit
GGGCCGCGCGGCGGAGCGGCTGGAGTGCGCGCCTCCTAGCGGAGCCGGGG10.7462686567164178No Hit
TGATTGGCTTCCCGGTACTGGTGCAGCAGGTCACTGACATCTGTACTTTC10.7462686567164178No Hit
ATCATGATGTCGCTGGCCATCGCCGAGCACATGGCCGCCAACGGCGTGAA10.7462686567164178No Hit
GACCTCAACAGCGTGACCATCAACGGCACCTTGGTACCCGCCCCTGAGAG10.7462686567164178No Hit
CAGAAAAGGTTCTGCTTCCTAACCTGTGATGCAGCCAGTTACCTTGGAGA10.7462686567164178No Hit
GTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGT10.7462686567164178No Hit
GTATAAAGCTTCAGTTATGCAAGACAAGTAAGGTCTAAAGATCTGTTGTA10.7462686567164178No Hit
CTGCAGCAGCGGCACTACAAGCCAAATCAGATGAGAAGGCGGCGGGTGCA10.7462686567164178No Hit
ATTTAAGTATCAACAGCCAAAACAGGCAAGGGAGCTAAATGTAACAAGTA10.7462686567164178No Hit
GGCTTGGTCAAGGTGATAAAGAACTGAGAGCCATTGGTGTCAGGCCCAGC10.7462686567164178No Hit
GTTTGTAATTACTGTGGCCCCTCAGAATGATATTTGGCCTCACGGGAGGA10.7462686567164178No Hit
GTTTCAGGCCGCAAACAATAGGAACCCGGAAGCGACCGGATTGATGGCCT10.7462686567164178No Hit
CTACAATATTATGGATTTATAAATTTCTCCTTAGAATTTTTAAAATTTTT10.7462686567164178No Hit
GGGTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG10.7462686567164178No Hit
GCTTGAGGAGCACGGTGGGGAGGCGGCGGCGGCAGAAGGACGAGGCCGTG10.7462686567164178No Hit
GAACAAGGAAGCTCATGATATGAAAAAAGCTTTAGGAAATACTGTCAGCT10.7462686567164178No Hit
GCGCCACCTGCTGCTGTGTCAGCGGCTGCACCGGCTCCTGGAAGTTCGTG10.7462686567164178No Hit
GAGTATATATTGAAGATACATTTGCAGAAGCATTCCCAATATGGGTTTCA10.7462686567164178No Hit
GGACGAGAAAGATCATTGAAATTCTGAGAAAACTTCTTTTAAACCTCACC10.7462686567164178No Hit
GTCAAAGAGATATTCCGCCAAGCCAGATTCGGGCGCTCCCATCTTGCGCA10.7462686567164178No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA10.7462686567164178No Hit
GGCTTAATTTGACCCAACACGGGAAACCTCACCCGGCCCGGACACGGACA10.7462686567164178No Hit
GCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCA10.7462686567164178No Hit
CTGTCAAGGAGTCCATAATGTTGGCCCACTCGTTTATAAGTTCCTCCCAT10.7462686567164178No Hit
GGGCTGAGGCGTGTTAGGGCTGGATCCGGCCATGGTCCTGTCTCTTATAC10.7462686567164178No Hit
ATGTCTTGTCTTAAAGTCTATTAATATAGCCACTCCAACTCTCCTATGGT10.7462686567164178No Hit
ACCTACCCGAGCGGCTGCCAGCTGCGCGCCGCCAGCCAGAGGGCCGAGAG10.7462686567164178No Hit
GTCTTCAAGCATTTCCAAGATCTTTGCTGCAAGGAGCATTGTACTCAGCA10.7462686567164178No Hit
GCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATAA10.7462686567164178No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA10.7462686567164178No Hit
TCACCAGACTTCAAGAATTTAGGGCCATCTTCCAGCTTTTTACCAGAACG10.7462686567164178No Hit
CTGCTGCTGCTGCTGCTGCTGCTGCTTTGGTCTTCTTAGTTTTGTTCTTT10.7462686567164178No Hit
GTCATGAAGATGCCCAAAATCAGGGCCCAGATGTTCAGGCACTTGGCGGT10.7462686567164178No Hit
TCAAAGATTAGGTTACAAAAAGGCTTGAATACTAGGAGGTAGCACTCTTT10.7462686567164178No Hit
ATGTGAAGATTCAGAGCCTTTCCGTCAGCCGGTAGATCTCCTTGAATATC10.7462686567164178No Hit
ACACCATGGAGACCATAGACTGGAAGGTCTTTGAGAGCTGGATGCACCAT10.7462686567164178No Hit
CGTATTTCCTGTCCAACATCCAGATGGCAAACGGTCTGCGCGAGTTTCTC10.7462686567164178No Hit
GAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCG10.7462686567164178No Hit
CCCCTCCGGTGAGCAGGGTCAGTGGAAGCCCCAACGGGAAAGGAAACGCC10.7462686567164178No Hit
GTACATGGGGCAGTAGCCGCCTCTGCCGCCGCGGAGCTTCCCGAACCTCT10.7462686567164178No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10.7462686567164178No Hit
ACATTATGTGTATTGTTAGATTTTCACATCTAAATTGAGAAGGGAGGAAA10.7462686567164178No Hit
GCTACTCTCGGGGGCTCTGGCCCTGACCCAGACCTGGGCGGGCTCTCACT10.7462686567164178No Hit
CCCAAGGTGCTGGGATTATAGGTGTGAGCCACCGCGCCTGGCCATGTGTT10.7462686567164178No Hit
GAGGCCTGCAGGGCTCGGCCGTAGGAGAAGGTCAGGGCCCAGGGCTTCAG10.7462686567164178No Hit
CCCATTCTCTCCTGCCTCAGCCTCCTGAGTAGCTTGGATTACAGGCACCC10.7462686567164178No Hit
GATCAGGGAAGCAGATATTGATGGTGATGGTCAAGTAAACTATGAAGAGT10.7462686567164178No Hit
ATCCGCAGACGTAAGGCCCGGCAAGCCAAGGCGCGCCGCATCGCCCCGCG10.7462686567164178No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTACAATGTTTATTTAAAAA10.7462686567164178No Hit
ATTTTCAACCACGTTACTGCTAAAAGAATTACTGAAATGAAGTGTATAAA10.7462686567164178No Hit
GGACTGCACAAATCATGGACTCTAAAAGTGTTTCCTTATTGGCTTATGAG10.7462686567164178No Hit
GCCAACCGTTGGGTCCCTACCCTCCTCCATACCCACCGCCTGCCCCTGGA10.7462686567164178No Hit
CCTCACGTAGTAGAAAGCGGTATCCTGATTAGTCTAACAGTTGTGTTAGA10.7462686567164178No Hit
ATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAA10.7462686567164178No Hit
CGCCCATGCCGCCCCATGCGAAGGCCAGCACGTAGGCCTCCGTCGGGTGC10.7462686567164178No Hit
TGACATCACTTACCAGGAGCCTCAGGGAACCCAGCCAGCACAGCAGCAGC10.7462686567164178No Hit
GATTGTGCCTACAGCTAAAATATCTGAAGATTCCAAAGTTCTGCAGCCTC10.7462686567164178No Hit
TGTTAGACACATTTGGAGTTAGTCACTCATCCCCAGTCTTTTCCCACTCT10.7462686567164178No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC10.7462686567164178No Hit
GAGGCTGAGGTGGGTGGATCACCTGAAGTCAGGAGTTTGAGACCAGCCTG10.7462686567164178No Hit
GGTGGCAGAATAGGGCGAGGAGCAGGGGCGTAGCCACACAGTGCAAAATT10.7462686567164178No Hit
TCAACGCAGAGTACATGGGACCCCAAGTGCCTGTGCCCAGAGGGCTTCAG10.7462686567164178No Hit
GCTACAGCGAGTCGATGTTCGCCATCGGCTGGCGCCATCGCTTCCAGGGG10.7462686567164178No Hit
GTCGTTGGAGAAAAGCTTGGTCAGGATGTCCGTCGTCACACCCATCTGCA10.7462686567164178No Hit
TTTCTGGACCACCCCGGGTCTGAATGGCCAGGTTGTCCCGGTCACCAGGC10.7462686567164178No Hit
GTTAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGA10.7462686567164178No Hit
ATCTTGATTTTCATGTCGTGTTAACCTTTTCCTTCCCCTCAGCCAAAGGG10.7462686567164178No Hit
ATCTGCCCTCTCCCAGTGGAGCCATATAGGCAGCACCTGATTCTCACAGC10.7462686567164178No Hit
AGAGCAGACCCCTGGCATCTGAGGATTTAAGAGTTCAACTGTTCTCAATC10.7462686567164178No Hit
GCTATGGATTGATTACCCTCCTCTTAGTTGGACTGGTTGGTGGATTTGCC10.7462686567164178No Hit
TGCCTGGACCACCTCATCCTTGGCCTGTGCCCAGGGCCCTGAGTTCTGGT10.7462686567164178No Hit
ACCATAAAGATACCTAAAACTGGGTAATTTATGAAGAAAAGAGGTACAGT10.7462686567164178No Hit
GATTAAAGGCCTAAATGTAAGACCTAAAACCACAAAAACCTTAGAAGAAA10.7462686567164178No Hit
TCCTAAATCTGTGGTCATATGTTTTCCACCAAATTTAGAACATTTTTAGC10.7462686567164178No Hit
CTCTAACGTGTGCCACCACCGGCGCGGCCCCTGGCTGAGAGCCACCAACG10.7462686567164178No Hit
TGTCTGCCTTGTGGTTGTCCTCCACAGGGATGCTCTTGTACTGGTAGTGA10.7462686567164178No Hit
GCTCATAGTAGCATTATTTGCAATAGCCAAAAGATGAAAACAACCCAAAT10.7462686567164178No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTT10.7462686567164178No Hit
GTGCTGAACTATATAGTGCATGGATGGTCTTGCCATTTATAACCTTTAGG10.7462686567164178No Hit
GATGCGCACAGCGCATAATCGGTGCATGGACGAGCAGACGCTGCGCATCT10.7462686567164178No Hit
CCATTTTCCGTATCTGCTTCGGGCTTCCACCTCATTTTTTTCGCTTTGCC10.7462686567164178No Hit
GTAGGAGGATCCCTTGAGGCCAGGAATTTGAGACCAGCCTGGGTAACAAA10.7462686567164178No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)