FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003655129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003655129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTAAAAGTACAAAAAATTAGCTGGGCATGGTGGCAGGCGCCTGTAGT34.225352112676056No Hit
ATATGCTGTACCTTTTTTGCTCATTAATAATCTAATAACATGATGTACTC22.8169014084507045No Hit
AGAAAAGGGAGGTCGAGGCACCTGTGTTGTGGAAGGAAAACACAAACGGC22.8169014084507045No Hit
CTCTCCACAGGGTCCCCCTCCTGCCATGGGACCCGAACCACATACAGTAG11.4084507042253522No Hit
GGGTGAGCCCGTCGACCTCGAGCGTGTACACCGACCTGGGGGTGCCATCG11.4084507042253522No Hit
GACATTGACATTCTTTTTTTTTTTGAGATGGAGTCTCACTCTGTCGCCCA11.4084507042253522No Hit
CTTAATGATGAAGTCATTGGTTTGACTCTCAGACAACTGATAGGATAATA11.4084507042253522No Hit
CTTCGGCATGACTCCAGGTGGACAAGAGGAAGGCAAGGGAGACCTCACTA11.4084507042253522No Hit
CCATCTATGAGCAATGGGACCTTACCACCCATTGAGTCTGTTGGTACCTT11.4084507042253522No Hit
ATTCCATTCCGTTCCATTGCACTAGTTTTGTTTCCATTCCTGTCCATTCC11.4084507042253522No Hit
CTCCTGGGCCCCTGTCTGTACAGTTCTGAAGTCCTACAAGAATGTTGAGA11.4084507042253522No Hit
TTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATG11.4084507042253522No Hit
CCGCAAGGGCTGTTAGCCGACGTGCGTGAAATTGCCCTGGGCAACAATGC11.4084507042253522No Hit
CAGCATCGCATCGCGCGAGTACGGCCGCATGCCCGACGGCCGCGTGGTCA11.4084507042253522No Hit
AGCTCATTGTAAGAGATGACACTGGCGTAGGTGGCACCTCCCCTGTCTCC11.4084507042253522No Hit
GAGCGAGACTCCGTCTCAAAAAAAAAAAAAAAAAAAAAAAGGAACCTATT11.4084507042253522No Hit
GTCCAAGGGGCACACATGGGGAGTTCTACACATCCCAGGGCACAGCCCTC11.4084507042253522No Hit
ATTCTATTTGATGCCATTCCATGACATTCCATTCCATTCGAGTCCATTCC11.4084507042253522No Hit
CCCAAAGTGCTGGGATTACAGGTGTGAGCCACTGTGTCCAGCACTTTCTT11.4084507042253522No Hit
AATTAAACCCAATCATGGCAACAAGGATCCAATCAAAGTACTGTTTTAGA11.4084507042253522No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11.4084507042253522Clontech Universal Primer Mix Long (96% over 26bp)
GATATATACATTAAACACACAATTACTATACATTGAGAAGAGTGCCATAA11.4084507042253522No Hit
CTGTGAGAGAGAGTTCGTCCCACCGGGAAACTGAGGCGGTGGGCGGTTAG11.4084507042253522No Hit
GTGCTTGTCAGTGACTTCCCCGACTGGATTTTGAACCCATCTTTCTGAAG11.4084507042253522No Hit
GGTGATTCACTCAAGAAAGAGGAATGTAGGGGCCCCGCCAGCGTGGATTG11.4084507042253522No Hit
GCGCCTACCTCGTCGGGTGTGCCGGCACGGCCTGCCGCCGAGCCTTCCAG11.4084507042253522No Hit
GTATTCACCACCTGCTAAGTAAAGAGCCCTTGGGCCCTGAACAACCAGCA11.4084507042253522No Hit
AAGTGGAAGTTAATAAGCTATCCAAGCTTACATATCAAAGCAGAGAGTGT11.4084507042253522No Hit
CCTTCATGGAGAGCACTGTTGGTTGGAAGCAGAGTGAAGGACAGGCCTGT11.4084507042253522No Hit
GCTGAGTATTTTGTGGACTGTCCCTCAATTTGAGTTCTCCTGGCGTTTTT11.4084507042253522No Hit
ACAAAGTGGGGAGCGGTCATGTGGGCCCTCAGCTCTCGACAGGAAGCTGT11.4084507042253522No Hit
TTCTTTTCTCTGCCGCCCCCCTCCCCCTGTTTTTTTTTTAAAAATAAATA11.4084507042253522No Hit
GAAAGAATATTTTAAGCCAAAAGAAAGAATAAAAAATGAAAATAGAGCAG11.4084507042253522No Hit
GTGTGATAGATCATTTGAGAGGCATAAATAATGATGATCTGTTATGGCTC11.4084507042253522No Hit
GAAGCGCTCTCTAGGGCAAGCAGATAGAGATCGACGAGGTCGATGATGTG11.4084507042253522No Hit
GTGTGTGTGTGTATAAAACATTTTCTTTATCCATTTATTAATTGATAGAC11.4084507042253522No Hit
CCCCCATAGTGCCCGAAACATTTGCCTCTTGATACTTATTTTCCTAACTC11.4084507042253522No Hit
CATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATGGGCGA11.4084507042253522No Hit
CATGTGAACAGCAGTTGAACATGGTTCAGTCGGTCCTGAGAGATGGGCGA11.4084507042253522No Hit
CTTGTCATGGCACGCAGGTCCCCTGCATAACCGTTAGTTATGCATGAAGC11.4084507042253522No Hit
GTCTGAGTCCCCAAACCTCAAAAGTAGGGAAGCCGCCAGTGTAGCCTTCA11.4084507042253522No Hit
GTGTTAGAGTGCTCACCCTCTACTTTTTTTTCTTCTTCTAAGGTCCTAAA11.4084507042253522No Hit
GTCTCCCACCTACCTGCCTGGGTCTCTGCCCCTCAGCTTCTCACAAGTGG11.4084507042253522No Hit
ATCCATTAGAATAGTAGCATTGAAATACAAGCTCAGTTTATATCTGTCTC11.4084507042253522No Hit
CAGCCAGCCAAGGCAGCCAAGCCTCCCAGCCAAAGCCAGCCAGCCTAGCC11.4084507042253522No Hit
TAGCAGCGCACCGCGGCGGCGCGCATCGCCTTGTGCTGCTCCTGGCGCTC11.4084507042253522No Hit
TCGCTATTCTAACTGGCGTGAGATGTTATCTCATTGTGGTTTTTATTTGC11.4084507042253522No Hit
ACTGCAGCCTCCACCTCCCAGGTTCAAGCGTTTCTCCTGCCTCAGCCTCC11.4084507042253522No Hit
GTCTCAGCTTACCGCGATGAGGGGGGGGGGCATCAACCAGTTGGTCAAAG11.4084507042253522No Hit
GATTTCGTTGGAAACGGGTTCATCTTCACAGAAAAACTAAACAGGAGCAT11.4084507042253522No Hit
AAATGAAATTGGGTTATTTTGACTGACACCCAGCAGCGCTGGCCATCTCC11.4084507042253522No Hit
AGCCCCATCTCTACTAAAAATACAAAAATTAGCCAGGCATGGTGGTGTAC11.4084507042253522No Hit
GCACAATGAAAAGGCCTGCCAGATTCATCATCGATAAATCATCTTGTTGC11.4084507042253522No Hit
TTCATGAATAATGCAACTGTAACATTCATGTACACATTTTTATGTGGACA11.4084507042253522No Hit
ATTTGTGTTGAAGAAAAATGTTGTATTAGTTTTAAGATCTCAAGGACCTA11.4084507042253522No Hit
CCTCTGCATGGTCGACCACCGGCTTGGGGTAGGTCTCACCGAGCTTGATG11.4084507042253522No Hit
CTGCCAGATAAACCAGTCACCTCCCAAATAACCCAGTCACCTCTCACATA11.4084507042253522No Hit
GAGATATTTTTGGTCTTTGCTTTTCTGATTAAAAAAAAAAAAAAAGGCAG11.4084507042253522No Hit
GGTGAGGGATAAAAGATGACAAATTGGGTTCAGTATGTACTGCTTGGGTG11.4084507042253522No Hit
GAATGAAGCTCTTGGGTCTGTGATCCCTCCATGAAGCAAGGCCCATGAGC11.4084507042253522No Hit
GGGTACAGTGAGCTAACTTGCCAAGGGTACAGTGAGCTAACTTGCAGGAG11.4084507042253522No Hit
TTCCAATCCATTCGATTCCATTCGATTCAATTCCATTTGATTCTCTTTCA11.4084507042253522No Hit
CCTGAATCAAGCTTAGGCTGAGCTCGATGAGTGACTACAATGTTGAATAT11.4084507042253522No Hit
ATATGCCTGGGAAGCCAAGAAGCAACATGGCGGAAGTGGAAAGTTAGTAA11.4084507042253522No Hit
CAATAACTGAGCCATAATGGGTCCAGTGGTTCCTACCAGAATATTTCTCA11.4084507042253522No Hit
GATTACAGGCATGAGCCACCATTCCCAGCCAGGAGAGAGATCTTAAATTA11.4084507042253522No Hit
GATCACGCCACTGCACTCCAGCCCAGGGGATAGAGTGAGACCCTGTCTCA11.4084507042253522No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)