FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003655130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003655130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAACAGTCAACCCTACTTTCTAATGATAGCAATCTCAGGCCTGCTATG34.225352112676056No Hit
TTTCCAGCCTCCCTGCCGCTTTGCGCTGGGGTCTCTGGGCCCGAGGCGCT22.8169014084507045No Hit
GAGTACATCATGTTATTAGATTATTAATGAGCAAAAAAGGTACAGCATAT22.8169014084507045No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT22.8169014084507045No Hit
ACTTGGGAGGCTGAGGCAGGAAAATCGCTTGAATCCGGGAGGCGGAAGTT11.4084507042253522No Hit
CACTTTAGCAAACCTTCTCTCTTCCCTCAAGAGAGAGAATCTTCAAGATG11.4084507042253522No Hit
TTCCAGCACTTTGGGAGGCTGAGGTGGGCAGATCACCTGAGGTCAGGAGT11.4084507042253522No Hit
AAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGA11.4084507042253522Clontech Universal Primer Mix Long (95% over 21bp)
GGTCATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCG11.4084507042253522No Hit
GATCACGCCACTGCACTCCAGCCTGGTGACAGAGTGAGACTCCGTCTCAA11.4084507042253522No Hit
TTTATACACAGATCATCGTTACATGTGTCACAACTTATACACCAAGCTAG11.4084507042253522No Hit
GCACTCTGGGAGGCTGAGGCAGTCAGATCACCTAAGGTTGGGAGTTCGAG11.4084507042253522No Hit
AAACAATAGTGATTATGTGGAGTGCAGTCAGGGCACGTTCAGATCCGGAA11.4084507042253522No Hit
AAATAAATGAAATGACAAAAGATACTTACATGAGGCTAAAAAACACTTAT11.4084507042253522No Hit
GAGTTAAACTGACTCTGCAGGATTCTTTCTTAAGTAAGAATTGGAAAAGA11.4084507042253522No Hit
CACACCACCAGGATGGACATAATAAAACAAACAAAAAACAGATAATAGCA11.4084507042253522No Hit
CTTTTCCACATGCCAGCTACAGGCCCAGGTTTCCCAAGTTTCTGGAGCCC11.4084507042253522No Hit
TAGAATAGACGGTAATGGAATGGACTCGAATAGAATGGACACGAATGCTG11.4084507042253522No Hit
CTATATACGTAAAAGGTAGCCTGGGTGTTGCGTTTACTTTTAGCATTTAA11.4084507042253522No Hit
CACAGGAGCTGCTGTGGTTGACTGAAACCTTTTAGATCTGAACTGGGTGA11.4084507042253522No Hit
GAGCTGAGATCACACCCTTGCACTCCAGCTTGGGCAACAAGAGTGAAACT11.4084507042253522No Hit
GGCTACGCCCCCACCCTCACGATCCGCGACGCCGCCGGCCAGGTCGTGTA11.4084507042253522No Hit
GGGTATTTCACTTGCCGGCGCGTACTCAATGGCCACAGGCTGTGCCGGCT11.4084507042253522No Hit
GTGAACTTGTACACTTAAAAATTTTTAAAGAAAAAGATGGTGGTTGAACA11.4084507042253522No Hit
CACGTGGCCCGGCCGTCACACACGGCCCCAGGTGGGCCTCTTATTAGCTC11.4084507042253522No Hit
ACACAGAAAACATTCGATGTACTTCAAGCGAGTGTCTGGGGCACCCTCCC11.4084507042253522No Hit
GTGCATGACCATGAGTTCATGAATTTAGCTCTTTAAACTCCAGCTTCTTC11.4084507042253522No Hit
ACAAAATGCCTTAGACTGGTAAGTTATAAACAATAGAAATTTGTTGCTCA11.4084507042253522No Hit
ATTAAATTGTTCTCTTTTAGAATAGCTCAACAAAGCCTTGTGCAGGGAGG11.4084507042253522No Hit
CCAGGAGGCAACATTCCTCTCTGCCTTAGTCATCCCAGGGCAAGGTACAG11.4084507042253522No Hit
GAGTGAGATGACTGGGAGGAAGAGGAGGATCTGGACCTGTCTCTCGATCT11.4084507042253522No Hit
ATGTGGATGCCATAAAATTTCTTTATTTGCTTTCAGTTAGCCCATAGGTT11.4084507042253522No Hit
CAGTATGGAAAAGCCTCAAAAAACTAAAAATGGAACTACCATGTGATCCA11.4084507042253522No Hit
CCCCTTGCCTGTTAAGGTATTATAATTAAATCTTTTCCAATCTAATCTAT11.4084507042253522No Hit
CAATGGAATGGAATGGAATGGAATGGAACGCAGAGTGGAATGCATTGTAA11.4084507042253522No Hit
GCAGGGAGCTTGCCCCTGCTGGACTCGCTCAACCCCGCCCTGCTTCTTCC11.4084507042253522No Hit
GCACTTCCAGGCCCTGGGTGGGGTGGGGTGAGCACACTGCCACCCTCAAA11.4084507042253522No Hit
GTGCAAGGACCTGGGTCTGGACTGGCGCCGCGGCGAGGCCTACTTCGCGC11.4084507042253522No Hit
AGGGTGTACTCGGTGACCACGCGGCCGTCGGGCATGCGGCCGTACTCGCG11.4084507042253522No Hit
TCTGTTAAGCGGTGATAATTAATACCTCTCCAAAGCCTGGGAAGGTAGAA11.4084507042253522No Hit
ATCAGTAGTAGATACCTGTCTTATATTTCAGATGTTGGTTGTTTGTGTCT11.4084507042253522No Hit
GGTTATGTGAGGGGTGCCCGGGTTATTTGGGGTGATTGGTTTATCTGGCA11.4084507042253522No Hit
GGGAATCTGGTCGGTGGGAGCAAGGGAAAGCAAAAAGAGAAATTAGATAA11.4084507042253522No Hit
CTCTCACACACTCTACAGCCAGGCTGGAGGGCCAAGCTTCTGAGAGAAGT11.4084507042253522No Hit
CTATTACTCCAGGCTGGGCACTGGCAGCTGAGGGTGCAGGAACTGTGAGG11.4084507042253522No Hit
GGGCTAATATCCAGAATCTACAAAGCACTTAAACAAAATTACAAGAAAAA11.4084507042253522No Hit
GCTCTTCGGTAGTGTCGCCATCCACCCGCATGGGGGCAGCAGCGCTCCAC11.4084507042253522No Hit
GCTAGAAAAAGCAGGTGGAAGAAGGTGAAAGAATCTGACTTGCTGAGTCT11.4084507042253522No Hit
TCCCAGCACTTTGGGAGGCTGAGGCAGGCGAATCAGCTGAGGTCAGGAGT11.4084507042253522No Hit
TTATTAGCATTCCGTGTTTGATGATTTGCCACTCTAAACAATTTCTACAA11.4084507042253522No Hit
GGTAGGAGGTACCCCTTTTCCAGCCGGGATTTCCTCCCCCATATGGAGGG11.4084507042253522No Hit
ATTCTCCCTCCTTTTATTTAAATGGTGGCTGGGTCCCTGACAAGGTATGT11.4084507042253522No Hit
GTCCGCCGCGCCGCGCAGCACCTGGCGGTCGACCGTGGTCGGCTTCGCGA11.4084507042253522No Hit
CTCCAAGACTCACAGTACAGCCACAATGCCACCTCCTATTAACCCCATCC11.4084507042253522No Hit
CCCTTTTGATAACATCAGGCAGAGTTGAGAGCCTGGGGACAGGAAGTAGG11.4084507042253522No Hit
ATATAAACTGAGCTTGTATTTCAATGCTACTATTCTAATGGATCTGTCTC11.4084507042253522No Hit
TTTTCATATGACCCAGATGGTGTAGCCGCTTGACTGCAATTTTCCTTTAT11.4084507042253522No Hit
GATCGTCGCAGACCATGCGGACGGCGCGCACGGCGACCAGATCCCGATGT11.4084507042253522No Hit
GAGTTGAACATTCCTATAGATAGAGCAGGTTGTAAACAATCTTTTTGTAG11.4084507042253522No Hit
CCTACCAGATCTCCAAGCGTGGCAATGCGCTGCGCGTGATGGCGGAAGCC11.4084507042253522No Hit
GTAATGGAATGGATTGGACTGGAATGCAATGGACTCGAATAGATTGGAAA11.4084507042253522No Hit
CTCAGCAACTGGGAAACGGGGGAGGGAGAAATTTTGGATTGAGGCTGCCA11.4084507042253522No Hit
CCCCTTTATTTTTTCTCTGCTTTTCTCAAGCAGAAGGAGTTTTGTCCCAT11.4084507042253522No Hit
TCTCTGCCCTTTGTTCTGGAGGCTTGGCTGACTTGGAACACTGCGCAGGG11.4084507042253522No Hit
GTGCCTGGAGAGCACATGGGACCCTGGAGAGAAGGGAGTGACGGCCTCAC11.4084507042253522No Hit
GGGTGGCTTGGCTGGCTTGGCTGGCTGGGTGGCTTGGCTGGCTAGCTGGC11.4084507042253522No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)