FastQCFastQC Report
Tue 10 May 2022
EGAF00003655176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003655176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485810
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGT10640.219015664560219No Hit
GACAGGATCCTCCCTGTCCCATGGCCTAAATGCCTCTCAGAACTCACAGA10420.21448714518021447No Hit
GTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGG9560.19678475124019676No Hit
ACTTCAGACATCAAATCCTGCAGACAAGGGGAGCCCTCAGTCTGCAGGGC9090.18711018711018712No Hit
TCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC8950.18422840205018423No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC7630.15705728577015704No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG6690.1377081575101377No Hit
GGACGCTCTCTGGCCTTCGGCCTCCATCCTGAGGCTCCACTTGGTCTGTG6600.13585558140013584No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT5970.12288754863012288No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCA5740.11815318746011816No Hit
GTACATGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGG5600.11527140240011527No Hit
GCTCCACACAGTCAGGGCTTCCCTGAAATCTGCCTCTCTCTGTCATTATG5430.11177209197011177No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC5370.11053704123011054No Hit
GGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCT5340.10991951586010991No Hit
CTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGG5250.10806693975010807No Hit
CCCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGC5240.10786109796010787No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAG5150.10600852185010601No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG5110.1051851546901052No Hit
ATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTG5010.10312673679010312No Hit
TTTCTAGTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT4940.10168584426010169No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT4920.10127416068010128No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA4890.10065663531010065No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)