FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003655289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003655289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAA33.1578947368421053No Hit
GGCTGTAGTGCAGGTGATGAGACTATTTTATAGGATCTGTGTATGGGGGC22.1052631578947367No Hit
CTTTTGAAAGTCAATCAACACATAATGATGATGATAATGACTGTCTCTTA22.1052631578947367No Hit
CGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGTGA22.1052631578947367No Hit
ACCTATGGCATCAAGTGGAAGCGTCCCACGGAACTGCTGTCAAACCCCCA22.1052631578947367No Hit
GCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAAACGGTAACGCAGGTG22.1052631578947367No Hit
GTTGAGTGGAGTTTATTGTACATAAGACTTGCATTTGTAGTTAGAGAAAA22.1052631578947367No Hit
TCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGT22.1052631578947367No Hit
GTGTTGGACACTTACTGAAGCAGAGAAGATGTCCTTTGAAACTCAGAAAA22.1052631578947367No Hit
ATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCAC22.1052631578947367No Hit
AAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGAC11.0526315789473684No Hit
CCACCTGGCTCAGGAGGGTGAGGCGGGAGAATCACTTGAACCCTTGAACC11.0526315789473684No Hit
GAGTACTTTTTTTTTTTTTACAAAAGAAAGGCCTTACATATTTATTACTG11.0526315789473684No Hit
AAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC11.0526315789473684No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACG11.0526315789473684No Hit
GATTAAGATGAGATCAAACATCATAGCAGAACATTAGCAAATTTTATCTG11.0526315789473684No Hit
ATGTTAATAGGGTGTTGAGAAATAGCGAAATGAGATTAAAGACATGGGAT11.0526315789473684No Hit
ACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAG11.0526315789473684No Hit
GAGAGGAATTACGGGAACAGAAAGAAAAGTCCGAAAGAGCTGCTTTTGAG11.0526315789473684No Hit
TCTTCAACATTTGCGGTAGAACTAGATGCGCCATCGTTAGAGAAGCCATA11.0526315789473684No Hit
GTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGG11.0526315789473684No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11.0526315789473684Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCTCACGACGGTCTAATCCCAACTCACGTTCCCTATTAGTGGGTGAACA11.0526315789473684No Hit
GACCGATATGTCAAAATTAAGCGTAACTGGCGGAAACCCAGAGGCATTGA11.0526315789473684No Hit
AAGGGAGTCCATTACCACCAGACATGCCTTACAAGAGCTCCTGAAGGAAG11.0526315789473684No Hit
ACCTCTGCTTCTGAGGTTCCTCAGCCATGAGCAGCCTTAGCATGGCCTTA11.0526315789473684No Hit
CGATGTGTCCCGTATTTGGTAAACCAAATCCCACAAACCAGTGCCCAAGG11.0526315789473684No Hit
CCCCCCCCTCCACGCGCCCCGCGCGCGCGGGAGGGCGCGTGCCCCGCCGC11.0526315789473684No Hit
ACACAACAGACATCCCCTGACAAGGTGGATTATGAATATAGTGAACTACT11.0526315789473684No Hit
ACCAGATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACC11.0526315789473684No Hit
TATGTGAGATGGGTCTCTTGAAGACAGCATATGGATCTTGCTTCTCTATC11.0526315789473684No Hit
CCTGGGCTTAAGCAATTCTCTTGTCTCAGACTCCCGAGTAGCTGGGACTA11.0526315789473684No Hit
GTGTGGGAATCTATCGCCAGACCCCTACCCACCGATGACCCAGTCGGCGG11.0526315789473684No Hit
GAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAG11.0526315789473684No Hit
GTGCTCTGCTCTCAACCTCTGCGAGTCTCTCCAGAAGCACCTAGCAGAGC11.0526315789473684No Hit
GGCGAGGTTTTAACCATGTTGGCCAGACTGGTCTCGATCTCCTGACCTCG11.0526315789473684No Hit
CTCCTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTT11.0526315789473684No Hit
GTCCTCAAGTAAAGCCACATAACCATTTAAAGTAAATGAATTGAACATGA11.0526315789473684No Hit
CAGGTGGACAGTGCCATTGTTGCTGCAGTGAGAAGGGTCCAGGTTTACCA11.0526315789473684No Hit
TAGTGAGACCCTATCTATTAAAAAATAAAGTAAATAATAAAGTTTCCAAA11.0526315789473684No Hit
GTCATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG11.0526315789473684No Hit
CTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAG11.0526315789473684No Hit
GGGTATAGGTTTGTCATGTAAGGGAGTAGTTGATGATACTGCTACAAAAA11.0526315789473684No Hit
AGACTTGAGAGGACTAAGAGATTTTCTTCTCTCTTAGGGGTTGTTTGTAA11.0526315789473684No Hit
TCTCACGACGGTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAA11.0526315789473684No Hit
GGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGA11.0526315789473684No Hit
GTATTCATCTCGCGACGCAGCGTGTCGCGCAGCTTCGCGTCGAGCGCGGT11.0526315789473684No Hit
ATCTGTGTTTGTGATGGGTTCACCAGGGTCGGATTTTTTGTTCGTTTGGG11.0526315789473684No Hit
CAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTT11.0526315789473684No Hit
GTGTAACTGTCACTTAAAATGAGGTGTCTTCCTCTCCGCAACATCTGACT11.0526315789473684No Hit
TTGCTGCGGTTTGGGGATCGTCGGACCAACCCGCAACAATGAGGGAGTGC11.0526315789473684No Hit
TGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAAT11.0526315789473684No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA11.0526315789473684No Hit
TGGGAGGACTGCTTGAGCCCAGAGTTGAAGACCAGCCTGGGCAACATAGT11.0526315789473684No Hit
ACCAAAAAAATAGAGTATAATGGCCAGGTGCGGTGGCTCATGCCTGTAAT11.0526315789473684No Hit
TAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCC11.0526315789473684No Hit
TTTCCTGACTTTGTAATGATCACCATTCTAACTGGTGTGAGATGGTATCT11.0526315789473684No Hit
GAATGGCGGCGGTGATGATGTCACCGTTGCCCGGCGGGGTGTCAATGATG11.0526315789473684No Hit
TGGCATCCCTCATCAGGGTTGCCAGATGGTGATAACAGTGTAAGGCCTTG11.0526315789473684No Hit
CTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGAT11.0526315789473684No Hit
GACATAACGATAGCGACTCTGTCTCAGGGGATCTGCATATGTTTGCAGCA11.0526315789473684No Hit
GTGTGTAATATGTCCCTTGAAAGAATGCAGAAGCATTAATATAAAAATAT11.0526315789473684No Hit
GCGTTATTCCCATGACCCGCCGGGGCGCGTCCGGGGCAACAAACTCAGTG11.0526315789473684No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA11.0526315789473684No Hit
GATATCTGCACCAGCCTGCAAAGCTTCCATGAACGCTTTGGTCCCAGACT11.0526315789473684No Hit
CCATGGCTCTGGAGAAGTCTCTTGTCCGGCTCCTTCTGCTTGTCCTGATA11.0526315789473684No Hit
GTCGTTTACAGTGTCTCTCACAAAAGAAGAACTCCCTCTGGCAGGGGATG11.0526315789473684No Hit
GGTAACTATGACTTCTTCGAGAAAGAGCGCGATATCAGAAAAGACCAACT11.0526315789473684No Hit
TAAAAAAAACATTTGTTTTGTTGTTTTTGTCGTTGTTATCCTTTATCCGT11.0526315789473684No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT11.0526315789473684No Hit
TCCGATTACACGTATCTGAACATAACCTTCTTCCTCCTCTACAGAAAGAG11.0526315789473684No Hit
GTCCAGGTGCCACAGGCAGCCCTGGGACATAGGAAGCTGGGAGCAAGGAA11.0526315789473684No Hit
AAAAAAAAGAGAAAAGAAAAAAGAGAAAGTCAAACAGCTCCAGATGGAGA11.0526315789473684No Hit
GGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCAATAATCAAGAACGA11.0526315789473684No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCT11.0526315789473684Clontech SMART CDS Primer II A (96% over 26bp)
GCGCGGCGCCTGGCCGCCGGTCGTGCCGCGGGTGCCGGTTGTGCCCGAAC11.0526315789473684No Hit
GCGCGTGCCCCGCCGCGCGCCGGGACCGGGGTCCGGTGCGGAGTGCCCTT11.0526315789473684No Hit
CCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAAC11.0526315789473684No Hit
GACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACCCAAC11.0526315789473684No Hit
ACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCC11.0526315789473684No Hit
ACTGAATTCATTCTCAAGAGTATAAGTCAATTCCTGAGAATTTAAATATT11.0526315789473684No Hit
GAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT11.0526315789473684Clontech SMARTer II A Oligonucleotide (95% over 21bp)
GTATATACAAGTGTGTAGTCCTGTTGATGGGATGTTTCCCCGAGTTCTTT11.0526315789473684No Hit
GTATTACAGGCGGGACTTTCCTCTGTGTTGGCCCAGATGGTTACACCTAG11.0526315789473684No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)