FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003655290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003655290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAAC33.1578947368421053No Hit
GGGCCTTCTATAGGCAGAGCCTGGACAAGAGTTAGATCATCTCGGTGATC22.1052631578947367No Hit
CCCCCCTGTGTCCTCCAAATAGAATTTGGATACATTTATATATTACCTTT22.1052631578947367No Hit
CATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTG22.1052631578947367No Hit
GTGCACGAACACTAGCTTCCCGTCCTTGATGGGCAGCAGGTCATCCACGA22.1052631578947367No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGA22.1052631578947367No Hit
TCATTATCATCATCATTATGTGTTGATTGACTTTCAAAAGCTGTCTCTTA22.1052631578947367No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT22.1052631578947367No Hit
CTGTCAACAGAGTCTCCCTTTGACTAAGAAAGCTGTGTTTTTTTCTGCTT22.1052631578947367No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG22.1052631578947367No Hit
CCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATG22.1052631578947367No Hit
GTCCTGGCCAACATCCGCTCACCTGTCTTCAACCATTCCCTGTACCGCAC11.0526315789473684No Hit
GCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGC11.0526315789473684No Hit
GTTAAGCGACTCTCTGCCTCAGCCTCCAGAGGAGCTGGGATTACAGGTTC11.0526315789473684No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT11.0526315789473684No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC11.0526315789473684No Hit
CAGTTGTGGTAGGCACAGCTCGCCGCCCGCAGGGAGCCGATTAGCACAAT11.0526315789473684No Hit
AAGTTCCACTGAATTCCTAAGGAAAATACAACAATTCCGACACCATTTAA11.0526315789473684No Hit
GCCAGCGCCAGCGCGTCGCACTGGCCCGCGCACTGGCGGTGCGACCGCGC11.0526315789473684No Hit
TATCAACGCAGAGTACATGGGAAGAAGACACAAGGAATTGTACTGAAGAG11.0526315789473684No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG11.0526315789473684No Hit
GGTGGTGCAGTAACAGTACTTGGTGCTCTTTATTGCTTGCTAAATGAGGT11.0526315789473684No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGACATCCTTGGAT11.0526315789473684No Hit
GTATTGTAATTCTTTTTTAAAGTTATTCCAGTGACTTTCCAGCTTAAAAT11.0526315789473684No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT11.0526315789473684No Hit
GTGTCACTGCATGTGAGATGGGTCCCTTAAAGACAGCATATCAATGGGTC11.0526315789473684No Hit
GCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAG11.0526315789473684No Hit
CAACAGCCATATTAAAGATCCTGACAGCCCTGAATTAAAGATACCTGTTT11.0526315789473684No Hit
CTGTAAAGATCTAAAAAAAAGTGAAAAGACAAGAGTCAAATTAAAACTTC11.0526315789473684No Hit
TCTCAGTACACAATCATTTAATATTTCCCTGTCTTACCCCTATTCAAGCA11.0526315789473684No Hit
GAAGACTGATGCGTTCCTCCTCACTTGTTTTTTCGATAGTTTTTATTTGT11.0526315789473684No Hit
GACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGC11.0526315789473684No Hit
TCTCCTTGTCATTGAACTCGGCGACGCCGCGGGCCTCGCCGCCACGGTGG11.0526315789473684No Hit
GGTAGACACAGCTTGAGTAAACTTCTTATTTACCTTTTCCAAATGTAATG11.0526315789473684No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11.0526315789473684Clontech Universal Primer Mix Long (96% over 26bp)
ACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCCATTCGTGATGGG11.0526315789473684No Hit
GCCATAGGACGTGCACTTCGTCAAGCATGCCGCAGGCCGACACCAGGGTG11.0526315789473684No Hit
TATGAATATAAAGTTTGGTAGATAATGAAGGATTTTCAGGTTCCTGGTTT11.0526315789473684No Hit
GAATACCATCTATCTCACTGAGGCTCAGGTAACCTCCACATATCAGTTGA11.0526315789473684No Hit
GAATACTTGTTTCTTCCTACCGTCAGGGTGAACCCAGTACTTACCTAGAT11.0526315789473684No Hit
TACATGCAGGAGATGGTCGCCGACCACCAGAAAGCGGTCAGCGACTTCCG11.0526315789473684No Hit
GGGCAGAGGTGACACCTCCTCTAATCTCATGAGCCAGAGCTGGCACCATT11.0526315789473684No Hit
GAACTCTTAACAAATTTACAAGAAAAAAACAACCCCATCAAAAAGTGGGT11.0526315789473684No Hit
GCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCC11.0526315789473684No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT11.0526315789473684No Hit
AGTAATGACTGTAGCTCCTCAGTAGCAGAGAGCAGTAGCCCACTTAGAAA11.0526315789473684No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA11.0526315789473684No Hit
AATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTT11.0526315789473684No Hit
TCTGAATGCAGTCCTGGCAAACGTCCCCATTATCCTTTGGCTGGGGCTTG11.0526315789473684No Hit
CTAAAAATACAAAATTACCCAGGTGTGGTGGCACATGCCTGCAATCCCAG11.0526315789473684No Hit
ATTGTAGGTCATTAGCATGCATATCGAGTTTGCCCTTACGTGGTGGGAGT11.0526315789473684No Hit
ATCCAAGGATGCAAGACGGCTTGCCGCAGAAACGGCATAACTGCTTGCCG11.0526315789473684No Hit
AAACGACATAGTTGCAACAGAAGTTCCCCTTTGGTTTCTTCTACAGCAAG11.0526315789473684No Hit
GCACAATTCTCCGAGTGCTTTCAAGCAACTTCGGGAGGCAGTGACTAAGA11.0526315789473684No Hit
GGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCA11.0526315789473684No Hit
GTCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCCTGGGCGGGATTCTGAC11.0526315789473684No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGT11.0526315789473684Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGCTGGAGCTCCAGTGGGCCAGGAGCAGAAGAGAAAGATGCTAGCTGCT11.0526315789473684No Hit
AACGGATAAAGGATAACAACGACAAAAACAACAAAACAAATGTTTTTTTT11.0526315789473684No Hit
GTACTGAGATGTATGCTAAAATATGTTTTTAAAACACCTGAATAATTAAT11.0526315789473684No Hit
ATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG11.0526315789473684No Hit
ATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACG11.0526315789473684No Hit
ATAACTTTTGTTCCACGACCCATAGGTTCACCTGTGTCTGTCCTCACTGT11.0526315789473684No Hit
GGTTTGCACCAACCCTGTGTCATATTCCGGCGCCTCATCATTTGGTTACA11.0526315789473684No Hit
GGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATGTTTTC11.0526315789473684No Hit
GAAGTTCTTTGATCCAGAGGTGGGTGCAGCTGAAAGTAAACAGAATGGAT11.0526315789473684No Hit
GTACAGGTTTGTATCCTAGGAGCAATAGGCTATACCACATAGCCTAGATG11.0526315789473684No Hit
AATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATT11.0526315789473684No Hit
ATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGA11.0526315789473684No Hit
CCTCCAAGGCCAACCACCACAACACATCCACGCCACCCCAAACGAACAAA11.0526315789473684No Hit
ATATCAGGCCAATTATGGCTTCTCTAACGATGGCGCATCTAGTTCTACCG11.0526315789473684No Hit
TCGTTGTATGATATAGCATTTAGAAGTATTGTTCTGGCCTTGTGATGATT11.0526315789473684No Hit
CCGTGAAAGCGGGGCCTAACGATCCTTCTGACCTTTTGGGTTTTAAGCAG11.0526315789473684No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTG11.0526315789473684No Hit
CCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACA11.0526315789473684No Hit
GACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACCCAAC11.0526315789473684No Hit
AAGGTATAGTCTGCTATTTTATTTTTTTTGAAACAGGTTCTCATTTTCAT11.0526315789473684No Hit
GTACATGGGAAGCAGTGGTATCAACGGAAAAAAAAAAAAAAAAAAAAAAA11.0526315789473684No Hit
AACAAAAGCAAAATCGTCCCATTCCCCAGTGGATTCGGATGAAAACTGGA11.0526315789473684No Hit
TCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTT11.0526315789473684No Hit
GATCTCGGCACAGTAAGATTTGTTGCACATCAGCAGCACTTCCAGCTCCT11.0526315789473684No Hit
TTCTTATAGAAAAGAATTTCCAAACAAGAATTTCATATCCAGCCAAACTA11.0526315789473684No Hit
GTCTCACGACGGTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACA11.0526315789473684No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)