FastQCFastQC Report
Tue 10 May 2022
EGAF00003655379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003655379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1104350
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTACTACTGTGCGGCAGATCATGGCAGTGCGACAACCTTCGTGGGTGT51230.4638927876126228No Hit
CTATATTACTGTGTGAAGAGAAAGGGGTACTCTGCTCCGGAGGACTTCGA49570.4488613211391317No Hit
GTAGTAATCACTCTCATCGTCTTGTTGGATGTTCTTAATGATCAGGGATC32040.2901254131389505No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC29090.2634128672975053No Hit
GGCCTGAGTCGATCCCTGATCATTAAGAACATCCAACAAGACGATGAGAG28890.2616018472404582No Hit
ATATTACTGTGTGAAGAGAAAGGGGTACTCTGCTCCGGAGGACTTCGATC28620.2591569701634446No Hit
GCTCTATATTACTGTGTGAAGAGAAAGGGGTACTCTGCTCCGGAGGACTT25600.23181056730203287No Hit
GGTATCAGCAGAGACCAGGGAAGGGCCCCCGATTTGTGATGCGAGTGGGC25000.2263775071308915No Hit
GTAATATAGAGCCGTGTCCTCAACTCTCAGGCTGTCCATTTGCAGATACA21750.1969484312038756No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21320.19305473808122425No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT21220.19214922805270068No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20990.1900665549870965No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG20040.18146420971612262No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19070.17268076243944402No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA18990.17195635441662516No Hit
GTCTTGGGCTCAGGCCTGAGTCGATCCCTGATCATTAAGAACATCCAACA18840.1705980893738398No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA18610.1685154163082356No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG18460.16715715126545025No Hit
GACCAGGGAAGGGCCCCCGATTTGTGATGCGAGTGGGCCCTAGTGGGATT18320.16588943722551727No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT17580.1591886630144429No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17500.15846425499162403No Hit
GCCATACACTGGGTCCGCCAGACTCCAAGCAGGGGGCTGGAATGGGTGGC16790.1520351337891067No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG16690.15112962376058317No Hit
GTACATGGGGGAGAGACTGAAGAACCCAGCATTGCAGCAGCTCCACCATG16530.14968080771494544No Hit
GCCTTGGGCTGACTCAGGACGGTCAGCCTGGTCCCTCCGCCAAAAATCAC16230.14696427762937475No Hit
CACCTGCACCCTGAGCAGCGGCTACACTAATTCTAAAGTGGACTGGTATC15940.1443382985466564No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15880.14379499252954228No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15680.14198397247249514No Hit
GTACATGGGGAGAGACTGAAGAACCCAGCATTGCAGCAGCTCCACCATGG15590.14116901344682392No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG15460.1399918504097433No Hit
CCTCACAGGGTCCCTCTCCCAGCTTTTACTGACTCAGCCACCTTCTGCAT15420.13962964639833386No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG15420.13962964639833386No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15410.1395390953954815No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG15160.13727532032417258No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14950.13537374926427312No Hit
GATCATGGCAGTGCGACAACCTTCGTGGGTGTGATTTTTGGCGGAGGGAC14680.1329288721872595No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT14360.13003124009598407No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14150.12812966903608455No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14020.12695250599900396No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA13920.12604699597048036No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTG13450.1217910988364196No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT13120.11880291574229183No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT13090.1185312627337348No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC13030.11798795671662063No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA12950.11726354869380179No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12940.11717299769094944No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA12850.11635803866527823No Hit
ACTCAGGCCTGAGCCCAAGACTGAGAAGCGATCAGGGATGCCATCCCCCC12770.11563363064245936No Hit
GGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAGTATGC12070.1092950604427944No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12000.10866120342282791No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG11970.10838955041427084No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11790.10675963236292843No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG11710.10603522434010956No Hit
ATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGA11680.10576357133155251No Hit
TTACTACTGTGCGGCAGATCATGGCAGTGCGACAACCTTCGTGGGTGTGA11580.10485806130302892No Hit
GCCATGATCTGCCGCACAGTAGTAATCACTCTCATCGTCTTGTTGGATGT11570.10476751030017659No Hit
CAGTAATATAGAGCCGTGTCCTCAACTCTCAGGCTGTCCATTTGCAGATA11540.1044958572916195No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11540.1044958572916195No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC11360.10286593924027708No Hit
ATATAGAGCCGTGTCCTCAACTCTCAGGCTGTCCATTTGCAGATACAGCG11300.10232263322316294No Hit
CCTGCACCCTGAGCAGCGGCTACACTAATTCTAAAGTGGACTGGTATCAG11240.10177932720604879No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA11220.1015982252003441No Hit
CCTCAGTCTCCTCACAGGGTCCCTCTCCCAGCTTTTACTGACTCAGCCAC11130.10078326617467287No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCAGCACTAGAAGTCGGCGGTGTT11120.10069271517182052No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC11100.10051161316611583No Hit
ATTCTAAAGTGGACTGGTATCAGCAGAGACCAGGGAAGGGCCCCCGATTT11080.10033051116041111No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)