FastQCFastQC Report
Tue 10 May 2022
EGAF00003655380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003655380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1104350
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTACTACTGTGCGGCAGATCATGGCAGTGCGACAACCTTCGTGGGTGT52580.47611717299769096No Hit
CTATATTACTGTGTGAAGAGAAAGGGGTACTCTGCTCCGGAGGACTTCGA52320.4737628469235297No Hit
GTAGTAATCACTCTCATCGTCTTGTTGGATGTTCTTAATGATCAGGGATC32960.2984561054013673No Hit
ATATTACTGTGTGAAGAGAAAGGGGTACTCTGCTCCGGAGGACTTCGATC30170.2731923756055598No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC29340.2656766423688142No Hit
GGCCTGAGTCGATCCCTGATCATTAAGAACATCCAACAAGACGATGAGAG28820.26096799022049166No Hit
GCTCTATATTACTGTGTGAAGAGAAAGGGGTACTCTGCTCCGGAGGACTT28290.2561687870693168No Hit
GGTATCAGCAGAGACCAGGGAAGGGCCCCCGATTTGTGATGCGAGTGGGC25820.2338026893647847No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21890.19821614524380857No Hit
GTAATATAGAGCCGTGTCCTCAACTCTCAGGCTGTCCATTTGCAGATACA21670.19622402318105672No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG21610.19568071716394259No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20600.18653506587585458No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT20400.18472404581880744No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19640.17784216960202834No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA19080.17277131344229638No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA18790.17014533435957804No Hit
GACCAGGGAAGGGCCCCCGATTTGTGATGCGAGTGGGCCCTAGTGGGATT18780.1700547833567257No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG18190.16471227418843665No Hit
GTCTTGGGCTCAGGCCTGAGTCGATCCCTGATCATTAAGAACATCCAACA18110.16398786616561778No Hit
GCCATACACTGGGTCCGCCAGACTCCAAGCAGGGGGCTGGAATGGGTGGC17060.15448001086612034No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT17020.1541178068547109No Hit
GTACATGGGGGAGAGACTGAAGAACCCAGCATTGCAGCAGCTCCACCATG16730.15149182777199258No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16610.1504052157377643No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16270.14732648164078416No Hit
CACCTGCACCCTGAGCAGCGGCTACACTAATTCTAAAGTGGACTGGTATC16210.14678317562367005No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG15890.1438855435323946No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15840.14343278851813285No Hit
GTACATGGGGAGAGACTGAAGAACCCAGCATTGCAGCAGCTCCACCATGG15620.141440666455381No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG15450.13990129940689092No Hit
GCCTTGGGCTGACTCAGGACGGTCAGCCTGGTCCCTCCGCCAAAAATCAC15440.13981074840403857No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14700.1331099741929642No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT14650.1326572191787024No Hit
CCTCACAGGGTCCCTCTCCCAGCTTTTACTGACTCAGCCACCTTCTGCAT14570.13193281115588357No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG14460.13093675012450764No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG14280.1293068320731652No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13930.12613754697333274No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA13830.12523203694480917No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13740.12441707791913796No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC13600.12314936387920496No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13430.12160999683071491No Hit
GATCATGGCAGTGCGACAACCTTCGTGGGTGTGATTTTTGGCGGAGGGAC13260.12007062978222484No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT13200.1195273237651107No Hit
ACTCAGGCCTGAGCCCAAGACTGAGAAGCGATCAGGGATGCCATCCCCCC12960.11735409969665415No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG12780.11572418164531172No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA12420.11246434554262688No Hit
GCCATGATCTGCCGCACAGTAGTAATCACTCTCATCGTCTTGTTGGATGT12330.11164938651695568No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA12140.1099289174627609No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12130.10983836645990855No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC11970.10838955041427084No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTG11960.10829899941141848No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCAGCACTAGAAGTCGGCGGTGTT11930.10802734640286141No Hit
CCTGCACCCTGAGCAGCGGCTACACTAATTCTAAAGTGGACTGGTATCAG11720.10612577534296191No Hit
CAGTAATATAGAGCCGTGTCCTCAACTCTCAGGCTGTCCATTTGCAGATA11640.10540136732014307No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11640.10540136732014307No Hit
TTACTACTGTGCGGCAGATCATGGCAGTGCGACAACCTTCGTGGGTGTGA11550.10458640829447187No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC11520.10431475528591479No Hit
ATGCCATACACTGGGTCCGCCAGACTCCAAGCAGGGGGCTGGAATGGGTG11440.10359034726309595No Hit
CCTCAGTCTCCTCACAGGGTCCCTCTCCCAGCTTTTACTGACTCAGCCAC11350.10277538823742471No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC11330.10259428623172002No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT11260.10196042921175351No Hit
ATATAGAGCCGTGTCCTCAACTCTCAGGCTGTCCATTTGCAGATACAGCG11190.10132657219178703No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA11140.10087381717752524No Hit
GTACATGGGAAGGGCCCCCGATTTGTGATGCGAGTGGGCCCTAGTGGGAT11070.10023996015755875No Hit
GGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAGTATGC11050.10005885815185403No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)