FastQCFastQC Report
Tue 10 May 2022
EGAF00003655425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003655425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170801
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCATAGATTTATCTCGTATG9390.5497625892120069No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5760.3372345595166305No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5360.31381549288353117No Hit
ATACACATCTCCGAGCCCACGAGACGAGCATAGATTTATCTCGTATGCCG5160.30210595956698144No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCATAGATTTATCGCGTATG4260.24941305964250796No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3690.21604088969034138No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT3610.2113570763637215No Hit
ACACATCTCCGAGCCCACGAGACGAGCATAGATTTATCTCGTATGCCGTC3460.20257492637630928No Hit
GTACATGGGAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCC3370.19730563638386192No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC2960.1733010930849351No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2810.16451894309752285No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG2730.15983512977090297No Hit
ATACACATCTCCGAGCCCACGAGACGAGCATAGATTTATCGCGTATGCCG2700.15807869977342054No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2650.15515131644428312No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG2360.1381724931352861No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA2240.13114677314535628No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT2090.12236462315794404Clontech SMART CDS Primer II A (100% over 26bp)
GATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGG2020.11826628649715167No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG2010.11768080983132417No Hit
GCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACT1960.11475342650218674No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC1940.1135824731705318No Hit
ACACATCTCCGAGCCCACGAGACGAGCATAGATTTATCGCGTATGCCGTC1920.11241151983887683No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA1890.11065508984139437No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT1880.11006961317556688No Hit
GGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCC1870.1094841365097394No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT1830.10714222984642947No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)