FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003655444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003655444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAGCACTCTGCGCGCCCGCTCTTCTGCTGCTGTTTGTCTAC23.278688524590164No Hit
GCACCAACACGTGTGTTCTCCATTCCACCAATCACAGACCAGTATCTACT23.278688524590164No Hit
ACCCTGGGCCCAGTCTGGGATGTACAGGGCTCTGGGAAGGGGGCAGTCCT23.278688524590164No Hit
CAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGGCACG23.278688524590164No Hit
ATGCTACAGTACTGAGGGGTATGTGTCCCCAATGCACAGCCACCCGCACA23.278688524590164No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC23.278688524590164No Hit
GGCGCAATGACGACCTAATTGGCGCACAGTACTAGGCATCGTCATCCAAT23.278688524590164No Hit
TCTTTCCTTGGCCTGGGAGCATCCGTTTCTGACGCACATGTTTTGTACAT23.278688524590164No Hit
CACCCACACTGTGCCCATCTACGAGGGGTATGCCCTCCCCCATGCCATCC23.278688524590164No Hit
ATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCAGT23.278688524590164No Hit
GACAATGGCTCCGGCATGTGCAAAGCTGGTTTTGCTGGGGACGACGCTCC11.639344262295082No Hit
CCCAGGGACTTGCCCTGCTGCCTGAGTGGGGGCAGAGCAGCAGACCGCTC11.639344262295082No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC11.639344262295082No Hit
ATCCAGGGCCTCGTGGCCGGAGAAACTGCCCAGCAGATCTGTGAGGACCT11.639344262295082No Hit
GCTTCATATTCGTGAGGGCATCTAAAATGCCACTCCACAGCACAGGTGCT11.639344262295082No Hit
AGTCAGTACCTTTCAGCTGTGACTGGCGGAAGGTGGCCGGCACCATCTCA11.639344262295082No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA11.639344262295082No Hit
GAGGCGGACGGGCCCGCAGCGCCGTGGTGGCGGCCGGGGGAGGCAGCTCC11.639344262295082No Hit
GTGCAAGCGCAACGAGCTGGTGATCCGCGTGCAGCCCAACGAGGCCGTGT11.639344262295082No Hit
AACATAATCTGAGTCATCTTCTCTCTGTTGGCCTTGGGGTTCAGGGGGGC11.639344262295082No Hit
GTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTA11.639344262295082No Hit
GTGTCTCGAAGCTTACAGAGGAAGCTGATATTGAAGCCAAAAGCACCAGC11.639344262295082No Hit
CGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAG11.639344262295082No Hit
TCTCAACCACCGCAGCCTTTGCTGCAGCCATCTCCATGGTGGTGGCCATG11.639344262295082No Hit
AGTCCAGGGCGACGTAGCACAGCTTCTCCTTGATGTCGCGCACGATTTCC11.639344262295082No Hit
CAAGAAGTGGTTTCAAGGAACCGAAATGAGCAGGAGTCTGCCGTGCACCC11.639344262295082No Hit
CCTGCGAACTCTGGCTCAGCACGACCCAAAAGATGTGCAATGAAGTCTGT11.639344262295082No Hit
GGTGTGAAAGGCATCTCAGCGGCTGCCCCACCATGGCTACCTGGGCCCTC11.639344262295082No Hit
ACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCACAGCAGT11.639344262295082No Hit
CTCCCAGGCTCTTCTGGAGCTGGAGATGAACTCGGACCTCAAGGCTCAGC11.639344262295082No Hit
GGGGGCACCCCCCAGCACCATCCTTCCAAGGTCCACCGTGATCAACATCC11.639344262295082No Hit
GAAGATGCCACCGTCAACAAGATTGCGCTCGAGCTGTGCACCTTTACCCT11.639344262295082No Hit
ATACATGGCTTGCTGCCTGTTGTACCGTGGTGACGTGGTTCCCAAAGATG11.639344262295082No Hit
CTTAATATTCTTTCTCTCTCTTCCTATGGACTAGGCAGGAGCTGATCTCA11.639344262295082No Hit
GGCTCACCTGAAGTCCTATCACTGCCGCTCTGCCCCTCCCCATGAGCCAG11.639344262295082No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC11.639344262295082Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCATCAGGGCCTCTGGTGTGGAGGCCCGCCAGGCCCTCTTGAAGCAGGGG11.639344262295082No Hit
GCCCTGGGCTTCTACCCTGCGGAGATCACACTGACCTGGCAGCGGGATGG11.639344262295082No Hit
GACGGGCACTGGGCGACTCTGTGTCTCGCTGAGGAAAAATAACTAAACAT11.639344262295082No Hit
ATGTCAACGAGTCCCGCTATCACTCCTCGACCAAACTTTTCCCCTTTCCT11.639344262295082No Hit
GTGTCTGGGTTTCATCCATCCGACATTGAAGTTGACTTACTGAAGAATGG11.639344262295082No Hit
CTCTTTCCACTGTTCCTTATCTTTATCAGTCCCTAAATCTGGGTGCAGAG11.639344262295082No Hit
TGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGC11.639344262295082No Hit
AAATAGAGGAGGGGCTGGAGCGGGGAAACGCGCCGGGGCCCTAGCGCACC11.639344262295082No Hit
CCGTGGAGAGAAGCCGCCGCCCGGGCTGCTGAGAGGCCCGGAGAGGGACT11.639344262295082No Hit
ATAGGAATCCTCTTCCCCTTGTTCATCAAACACATTTAGTTGCACGCTCA11.639344262295082No Hit
TTTCTCATGGTTCTGGAGGCTGGGAAGTCCAAGTTTAAGGCATGAGCATA11.639344262295082No Hit
CTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCAT11.639344262295082No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11.639344262295082No Hit
TTGCTAGGGTGGCGCTTCCAATTAGGTGCATGAGTAGGTGGCCTGCAGTA11.639344262295082No Hit
GATCCATGGCCTGTTCGATCTTGTTGTCTATGGCCACCACGCTGGCTCCG11.639344262295082No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)