FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003655479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003655479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCA36.382978723404255No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT24.25531914893617Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG24.25531914893617Clontech Universal Primer Mix Long (96% over 26bp)
ATGATTGGGTCCCCAAGGCACGAACCATTTTGTCTTGTGATGGTTCTTAC24.25531914893617No Hit
GTTTTTATCTTCTAATTTCCCTATCTTCTCTATTCTTTTCTTCGCCTTCC24.25531914893617No Hit
ACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTG24.25531914893617No Hit
GTTCCTGATTGGAGGCTGTTTCTGAGTATTTTCCATTTCCTAGGCTGCTT24.25531914893617No Hit
CTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCATC12.127659574468085No Hit
GAGCTCTACCTGAAAAAACAGCTTCAGAGTCCTCGTCACCTCCACTCACT12.127659574468085No Hit
GAGCCGCATCGCCACGCCTTTCATCAGAGCGCTTTCCGATCAGACGGAAT12.127659574468085No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTGAAAA12.127659574468085No Hit
GCTCTGGTCCGTATTGCGCCGCTAGAGCGCCGCTAGAGGTGAAATTCTTG12.127659574468085No Hit
TCTTGTCATCTTCCAGATTTTAGAGGAAAGGCTTTTAGTTTTTCATTCAC12.127659574468085No Hit
CATACTAAATGGGCAAAAGCTGGAAGCATTCCCTTTGAAAACAGGCACAA12.127659574468085No Hit
GCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTT12.127659574468085No Hit
GGTCAAACAATAATGAAGCAAAAATAATTCAAAATGAATAAAACATCCAA12.127659574468085No Hit
CCACCACGACCGGCTAATTTTGTATTTTTTACAGAGACAGGGTTTCACTA12.127659574468085No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC12.127659574468085Clontech Universal Primer Mix Long (96% over 26bp)
GCGAAAGCTCAGCTCAAGCCAAAACAAACGTGGACCAGAAGAGTGTGCAG12.127659574468085No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC12.127659574468085Clontech Universal Primer Mix Long (96% over 26bp)
GGCCTGGGTTGCCAGGCCAGAAGACTCACTTCTTGCTTTTGAACGTGCCC12.127659574468085No Hit
TATTTGGATTGCAAAGTATAAATATGAACCAGGTTTGGAAATACAAGTTG12.127659574468085No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCCGTC12.127659574468085No Hit
GTCAAGATGCTCCTCAGAATCTCCCCAAAGCAATGATCACCAAGTCCAAG12.127659574468085No Hit
CTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTC12.127659574468085Clontech Universal Primer Mix Long (96% over 26bp)
GTCTTAAAAATGAGAAATTTCTCTCATTTACATCCTTGGCAAGAATGGGA12.127659574468085No Hit
GTTATAAAATAAACTATAGTAAGACTGTTAGAAAACAGAATTTTCAACAT12.127659574468085No Hit
CACCCTATCTGTGCAGTTTCCAGTTGGAGATTTCAATCGCTTTGAGGCCA12.127659574468085No Hit
GCCTTCTCGACCGCCTGGTGCGCCGCTACGCGGAGACCAAGGACTCGGGC12.127659574468085No Hit
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCA12.127659574468085No Hit
GCTCCAGGGCGGCGATCCGCTCGGTGGCCCGTTCGATCGCATCGGAGTCC12.127659574468085No Hit
CCTCTATATTTCACAGATCCAAAAAGCCACTGTGGAACCACAGAGAATGG12.127659574468085No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAT12.127659574468085Clontech Universal Primer Mix Long (95% over 22bp)
GGTGGGCGATGTGGTCGCGGAGTTGGTCTGCTCCGGTGAGGGGGGAGGAC12.127659574468085No Hit
AAAAACGAGGAAAGGCTGGAAAAACAGACAAACATAACAGGCATTCTGTG12.127659574468085No Hit
AAAAAAAAGAGGTTAAAAATGATGTGCTTTGTATCTGTCTCTTATACACA12.127659574468085No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG12.127659574468085No Hit
GGGCACTGCGGTGTGTAGATGCCTGGAGGGAGGGGGATGGCGCGTGCGGC12.127659574468085No Hit
GAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTT12.127659574468085No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)