FastQCFastQC Report
Tue 10 May 2022
EGAF00003655543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003655543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1623897
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAGGACTGTTGCCTCAAGTACAGCCAAAGGAAGATTCCCGCCAAGGT32720.20149061178141225No Hit
AGCTTAGGCTGCTCCATCCCAGCTATCCTGTTCTTGCCCCGCAAGCGCTC28740.176981668172304No Hit
GTCCTGCTGCCTCCTCTCATGCTCCCTGGGAGACTCTCCCTCCTCGGTCT27560.16971519745402572No Hit
CTCTGGACCTGGCCTGCTGTGGGCAGCTCAGCCATGCAGGGTAGAGCTGG23790.14649943931172973No Hit
GGGTAGAGCTGGGAATGCAGATGGGGTGGTTAAAGCAGGAGAAAGAGTGT23510.14477519202264677No Hit
GTACTGGGGAGCCGTATCAGGTCCAGGGTCCTGATGATTCTCCTTCAAGG22240.13695449896144893No Hit
TCCTTATCCTGGTTCTGGCCTTTGGCATCCCCAGGACCCAAGGCAGTGAT20470.12605479288403143No Hit
GCTATGTGCAGACCCAAAGGAGCTCTGGGTGCAGCAGCTGATGCAGCATC20320.12513108897916556No Hit
GTGATGGAGGGGCTCAGGACTGTTGCCTCAAGTACAGCCAAAGGAAGATT19000.11700249461634575No Hit
GGAGAAAGAGTGTGGCAAGGCCAGCATGGGGTGGCTGCTCCAGGGCCCCT18720.11527824732726275No Hit
CTTTAGGGGTCTGTGACCGCTCAGTCCTCTTGCAGCCTTTGGAGCCCTTT17830.10979760415839182No Hit
CCCCTAAAGGGCCATAGCCCAGTGAGCAGCCTGGAGCCCTGGAGACCCCA16890.10400905968789892No Hit
GTCTTGGAGGCCCCCCTGTCCTTCCTGCAGCCCTGGGCTGGTTTCTGTGG16750.10314693604335744No Hit
CCCTGGGAGACTCTCCCTCCTCGGTCTCTCTGGACCTGGCCTGCTGTGGG16670.1026542939607623No Hit
GTACATGGGACAGACCCCCAACTTGCAGCTGCCCACCTCACCCTCAGCTC16610.10228481239881594No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)