FastQCFastQC Report
Tue 10 May 2022
EGAF00003655544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003655544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1623897
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36420.22427530810143745No Hit
GCTCAGGACTGTTGCCTCAAGTACAGCCAAAGGAAGATTCCCGCCAAGGT34790.2142377256685615No Hit
GTCCTGCTGCCTCCTCTCATGCTCCCTGGGAGACTCTCCCTCCTCGGTCT25140.15481277445552274No Hit
CTCTGGACCTGGCCTGCTGTGGGCAGCTCAGCCATGCAGGGTAGAGCTGG23710.1460067972291346No Hit
GTACTGGGGAGCCGTATCAGGTCCAGGGTCCTGATGATTCTCCTTCAAGG23020.14175775926675152No Hit
AGCTTAGGCTGCTCCATCCCAGCTATCCTGTTCTTGCCCCGCAAGCGCTC22620.13929454885377582No Hit
TCCTTATCCTGGTTCTGGCCTTTGGCATCCCCAGGACCCAAGGCAGTGAT22480.13843242520923432No Hit
GGGTAGAGCTGGGAATGCAGATGGGGTGGTTAAAGCAGGAGAAAGAGTGT22360.1376934620853416No Hit
GCTATGTGCAGACCCAAAGGAGCTCTGGGTGCAGCAGCTGATGCAGCATC21590.1329517820403634No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18010.11090604884423087No Hit
CCCTGGGAGACTCTCCCTCCTCGGTCTCTCTGGACCTGGCCTGCTGTGGG17310.10659543062152342No Hit
ATCCTGTTCTTGCCCCGCAAGCGCTCTCAGGCAGAGCTATGTGCAGACCC17210.1059796280182795No Hit
CCCCAGGACCCAAGGCAGTGATGGAGGGGCTCAGGACTGTTGCCTCAAGT16960.10444012151016968No Hit
GGAGAAAGAGTGTGGCAAGGCCAGCATGGGGTGGCTGCTCCAGGGCCCCT16520.10173059005589641No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)