FastQCFastQC Report
Tue 10 May 2022
EGAF00003655631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003655631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences791861
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA37980.4796296319682368Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG22160.27984709437641203Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT18020.22756519136565637Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15310.19334201330789116No Hit
CTTATACACATCTCCGAGCCCACGAGACGACCATCTCTCGATCTCGTATG14110.17818783852216488No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG13860.17503071877513857Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAGAGTCACTCCTGCCTTCACCATGAAGTCCAGCGGCCTCTT13530.17086332070906385No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG12340.15583543071321862Clontech Universal Primer Mix Long (96% over 26bp)
GTCCAGGGAAGAAGAGATGTTGTCCTGACACTTGTGGCATCAAATGCCTG9980.12603222030129024No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9140.11542429795128185No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT8110.10241696459353346Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC8020.10128040148460399Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)