FastQCFastQC Report
Tue 10 May 2022
EGAF00003655632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003655632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences791861
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55110.6959554770344796No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA37390.4721788293652548Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG19930.25168558623293735Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT15980.20180309422992165Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAGAGTCACTCCTGCCTTCACCATGAAGTCCAGCGGCCTCTT14290.18046096474002382No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG13710.1731364469269228Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13700.17301016213704173No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG12860.16240223978703333Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC9130.1152980131614008Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT9050.11428773484235238Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCCAGGGAAGAAGAGATGTTGTCCTGACACTTGTGGCATCAAATGCCTG8330.10519522997091663No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)