FastQCFastQC Report
Tue 10 May 2022
EGAF00003655635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003655635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601524
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAAC90891.5109954050046215No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA68481.1384416914370832Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG56660.9419408036919559No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG40490.6731235993908804Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGAACGT35070.5830191314062282No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT32470.5397955858785353No Hit
GTACATGGGCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACT30490.5068791935151382No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30300.5037205498034991No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG25140.4179384363716161Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT24850.41311734860121957Clontech Universal Primer Mix Long (96% over 26bp)
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA24700.41062368251308345No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG24680.41029119370133194Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22730.3778735345555622No Hit
TATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGAACGTC22350.371556247132284No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT21700.36075036075036077No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT21310.3542668289212068No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT20210.33597994427487515Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGAACG19990.33232256734560883No Hit
ACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCC19460.32351161383419447No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT18200.30256481869385093No Hit
GAGTACATGGGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCA17550.2917589323119277No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCTGGCAAGGATCTCGTATG17100.2842779340475193No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC16910.2811192903358802Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGC16150.2684847154893238No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT15370.2555176518310159Clontech SMART CDS Primer II A (100% over 26bp)
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT14810.24620796510197432No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGAC14680.2440467878255897No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT14200.23606705634355404No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA14120.2347371010965481No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAG13930.231578457384909No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13780.22908479129677287No Hit
ACATGGGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT13540.22509492555575503No Hit
GTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGAACGTC13410.2229337482793704No Hit
CCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGT13260.22044008219123423No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA13250.22027383778535853No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT12830.21329157273857735Clontech Universal Primer Mix Long (96% over 26bp)
GGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGAT12740.21179537308569565No Hit
GCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCC11510.19134731116297937No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT11380.18918613388659472Clontech SMART CDS Primer II A (100% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGT11010.18303509086919226No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA10760.17887898072229869No Hit
ATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGAC10730.17838024750467146No Hit
GTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT10670.17738278106941702No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC10140.16857182755800268No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGA10120.1682393387462512No Hit
ACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGG10050.16707562790512098No Hit
ATACACATCTCCGAGCCCACGAGACTTTCTGGCAAGGATCTCGTATGCCG9970.16574567265811505No Hit
CATGGGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTT9970.16574567265811505No Hit
GTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG9660.16059209607596706No Hit
TATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGAACGTCT9650.1604258516700913No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC9630.1600933628583398No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAG9480.1575996967702037No Hit
GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG9390.156103497117322No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG8850.14712629920003192No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC8740.14529761073539876Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA8700.14463263311189578No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTA8590.14280394464726262No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT8510.14147398940025668Clontech SMART CDS Primer II A (100% over 26bp)
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC8460.14064276737087797No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT8370.13914656771799627Clontech Universal Primer Mix Long (96% over 26bp)
TCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATG8320.13831534568861759No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAG8300.1379828568768661No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCTGGCAAGGATCGCGTATG8160.1356554351946057No Hit
GTACATGGGCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGAT7970.1324967914829666No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTAA7820.13000312539483044No Hit
TGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCG7790.12950439217720325No Hit
GAGTACATGGGCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAA7690.12784194811844582No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA7540.12534828203030968Clontech Universal Primer Mix Long (96% over 26bp)
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG7520.12501579321855819No Hit
GGGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGA7520.12501579321855819No Hit
GTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTT7410.12318710475392504No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA7400.12302086034804928No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA6910.11487488446013791Clontech Universal Primer Mix Long (95% over 21bp)
GGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGG6890.11454239564838643No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC6760.11238121837200178Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGA6560.10905633025448692No Hit
GTACATGGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACT6450.10722764178985376No Hit
GGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC6380.10606393094872357No Hit
ACACATCTCCGAGCCCACGAGACTTTCTGGCAAGGATCTCGTATGCCGTC6320.10506646451346913No Hit
GCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT6250.10390275367233893No Hit
CACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCC6210.10323777604883595No Hit
ATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGAACGTCT6200.1030715316429602No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6120.10174157639595428No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)