FastQCFastQC Report
Tue 10 May 2022
EGAF00003655636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003655636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601524
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAAC100211.6659351912808134No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG71811.1938010785937054No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA60881.0120959429715188Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG60401.0041162114894835No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG39340.6540054927151702Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGAACGT37340.6207566115400217No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT37090.616600501393128No Hit
GTACATGGGCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACT32150.5344757648905114No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30750.5112015480679075No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT25650.4264169010712789Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG25150.4181046807774918Clontech Universal Primer Mix Long (96% over 26bp)
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA23820.3959941747960182No Hit
TATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGAACGTC23240.38635199925522506No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22610.3758786016850533No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG21840.3630777824326211Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCC21390.3555967841682127No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT20280.33714365511600536No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT18290.30406101834673266Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT17620.29292264315305794No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT17370.28876653300616434No Hit
GGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGAACG17090.28411168964164357No Hit
GAGTACATGGGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCA16930.28145177914763164No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT16670.2771294245948624No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGAC15930.26482733856005747No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC15610.2595075175720337Clontech Universal Primer Mix Long (96% over 26bp)
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT15330.25485267420751295No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT15290.25418769658400997Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA15090.2508628084664951No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA13620.22642488080276102No Hit
GTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGAACGTC13610.22625863639688526No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13430.22326623709112187No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT13350.22193628184411593Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGC13270.22060632659711No Hit
GGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGAT13030.2166164608560922No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG12650.21029917343281399No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAG12380.20581057447416895No Hit
ACATGGGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT12040.2001582646743937No Hit
CCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGT11760.19550342130987292No Hit
GTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT11650.19367473284523976No Hit
GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG11580.19251102200410958No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT11240.1868587122043343Clontech SMART CDS Primer II A (100% over 26bp)
CATGGGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTT11020.183201335275068No Hit
ACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGG10010.16641065028161803No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC9950.16541318384636355No Hit
ATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGAC9830.16341825097585466No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT9750.16208829572884872Clontech Universal Primer Mix Long (96% over 26bp)
CCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGA9560.15892965201720963No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGA9530.15843091879958238No Hit
GTACATGGGCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA9470.15743345236432796No Hit
TCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATG9200.1529448534056829No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA9060.1506174317234225No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAG9000.14961996528816804No Hit
TATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGAACGTCT8590.14280394464726262No Hit
GTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG8470.1408090117767537No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT8420.13997778974737501Clontech SMART CDS Primer II A (100% over 26bp)
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG8310.13814910128274183No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC8140.13532294638285422Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGGCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAA7830.1301693698007062No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC7790.12950439217720325No Hit
GTACATGGGCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGAT7590.1261795040596884No Hit
CACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCC7560.12568077084206117No Hit
GTACATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG7410.12318710475392504No Hit
GTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTT7370.12252212713042207No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC7310.12152466069516761No Hit
ACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGG7120.1183660169835285No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC6930.1152073732718894Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGT6870.11420990683663494No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA6840.1137111736190077Clontech Universal Primer Mix Long (96% over 26bp)
ACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCT6710.11154999634262308No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT6710.11154999634262308Clontech Universal Primer Mix Long (100% over 23bp)
GCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCC6610.10988755228386565No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6550.10889008584861119No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6420.10672890857222654No Hit
TCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTC6130.10190782080183002No Hit
ACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGAACGTCTGCCCT6060.10074410996069982No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)