FastQCFastQC Report
Tue 10 May 2022
EGAF00003655660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003655660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439443
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20890.47537450818422416No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA19860.45193574593291963Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTT13540.3081173212453037No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11630.26465320872104003Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG10300.23438762251304493Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGA9030.20548740109638794No Hit
CTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAG7220.16429889655768778No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG7210.16407133575913144Clontech Universal Primer Mix Long (96% over 26bp)
ATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCATCTTCC6640.15110037024141926No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG6490.14768695826307396Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGAT6090.13858452632081975No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT5840.13289550635691091Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5150.11719381125652246No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT4550.1035401633431412Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAGAGTCACTCCTGCCTTCACCATGAAGTCCAGCGGCCTCTT4480.10194723775324672No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)