FastQCFastQC Report
Tue 10 May 2022
EGAF00003655663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003655663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1888931
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTT101270.5361233417207933No Hit
TCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGA72190.3821738327127883No Hit
CTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAG65000.3441099754305477No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA57440.30408733828816403Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCATCTTCC55350.29302287907816643No Hit
GGATAGGAGCATCATCTTCCATCCCCATCCACCTCCGCTCTTTATCTTGA39990.21170704488411698No Hit
GCCTTTGTAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTT34210.1811077270689083No Hit
GAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGAT31890.16882564794584873No Hit
ATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAG31060.16443162825958174No Hit
GTATCAACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTG30830.1632140083465198No Hit
GTCATGAAGCTGGTAACTATCTTCCTGCTGGTGACCATCAGCCTTTGTAG29120.1541612689928854No Hit
GTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAGAAGCCTCTGGTC26360.13954983003614213No Hit
TTTCACGGGTCACTACATTTTAGGGCATTTGATCTATAATTGGTAGAACA26310.1392851300550417No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG25900.13711459021001826Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT25870.136955770221358Clontech Universal Primer Mix Long (96% over 26bp)
GCTTTATTTGTCCTTTTCACGGGTCACTACATTTTAGGGCATTTGATCTA25670.13589697029695633No Hit
CATTTACACACTTCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCC25280.13383231044437305No Hit
GGTCACTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCA25230.13356761046327262No Hit
GGTTTCAGGCAGGGAGGATAGGAGCATCATCTTCCATCCCCATCCACCTC24920.13192647058045No Hit
GGCTAAGGAAGTGTGTAAATGAGCTGGGACCAGAGGCTTCTGAAGCTGTG23290.12329725119657627No Hit
GATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCAT23200.1228207912305955No Hit
TGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTTTGCTA22790.12065025138557205No Hit
ATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCATC22440.1187973515178691No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22300.11805619157078792No Hit
CCTTTACCTCTGGACAACATTCTTCCCTTTATGGATCCATTAAAGCTTCT22280.11795031157834776No Hit
CTATCTTCCTGCTGGTGACCATCAGCCTTTGTAGTTACTCTGCTACTGCC22200.11752679160858708No Hit
TAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTG21540.11403275185806151No Hit
TATCAACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTGG21440.11350335189586067No Hit
TTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTTTGCTAGATTTTTCT21090.11165045202815774No Hit
GTACATGGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATT20320.10757407231921122No Hit
ACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTTTG19510.10328593262538442No Hit
GGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTTTGCTAGA19400.10270359266696348No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG19320.10228007269720281Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)