FastQCFastQC Report
Tue 10 May 2022
EGAF00003655672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003655672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2127341
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGG107480.5052316483347052No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA101970.4793307701962215Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAG86270.40552971996497034No Hit
GAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGG82900.3896883480363515No Hit
GTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGC66030.3103874743165294No Hit
GGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGA64910.3051226860197777No Hit
GGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCC62320.2929478630835395No Hit
ATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCA60940.2864608917893276No Hit
GTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGAGGGAG55740.26201723184012343No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG53290.2505005074409791Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52110.24495367691404435No Hit
TAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCT50320.23653941704691442No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT49850.2343300862438133Clontech Universal Primer Mix Long (96% over 26bp)
GGACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCATC49220.23136864282689049No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45820.21538624978318005No Hit
GTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCT43860.2061728702638646No Hit
GAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTG38940.18304540738884834No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG37820.17778061909209666Clontech Universal Primer Mix Long (96% over 26bp)
GGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCA36730.17265685191043656No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG36700.172515830795345Clontech Universal Primer Mix Long (96% over 26bp)
GCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTC35880.16866125364950893No Hit
GTACATGGGACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCA35450.1666399509998632No Hit
CTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTC34800.16358449350621268No Hit
GATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTCATGGACACAC34150.16052903601256216No Hit
GATCTGCCCGAGCTTTCAGCGTGTCATCGAAACTCTCCTCATGGACACAC32960.15493519844726353No Hit
AAACCCTCCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTT32490.15272586764416235No Hit
GCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTT31540.1482601989995962No Hit
CTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGA31300.14713203007886372No Hit
CACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTT29550.1389057983651892No Hit
ATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGT29130.13693150275390736No Hit
AGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAG29080.13669646756208806No Hit
GAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAG28790.1353332634495363No Hit
CCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTCTCTGCTGC28700.1349102001042616No Hit
GTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGAGGGTG28410.13354699599170985No Hit
TCTAAATGCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTT28100.13208977780243036No Hit
CCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACAT27460.1290813273471437No Hit
GGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGA27400.12879928511696057No Hit
TTTCAGCCCTGATCAAGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGC26520.12466266574094138No Hit
ATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTT26180.12306442643657035No Hit
TGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGC26010.12226530678438484No Hit
AAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAA25930.12188925047747398No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25730.12094910971019691No Hit
ACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCT25540.12005597598128367No Hit
GATCAAGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGCTGGTGGACAC25440.11958590559764515No Hit
CTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCA24380.11460315953107658No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC23990.11276988503488629Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAG23850.11211178649779231No Hit
GTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTT23740.11159470907578992No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23550.11070157534687668No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT23100.10858625862050326Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACT22860.10745808969977076No Hit
GCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGCTTTT22680.10661196300922138No Hit
AATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTT22290.10477868851303106No Hit
TAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTCTTC21760.10228731547974679No Hit
GAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTC21530.10120615359737813No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)