FastQCFastQC Report
Tue 10 May 2022
EGAF00003655687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003655687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948703
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA70110.7390089416814324Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG37890.39938737413078695Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30280.31917259669253706Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG26260.2767989560484156Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG24990.2634122586309941Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23240.24496602203218498No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT17320.18256503879507074Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGCATCTTCTATCTCGTATG16550.17444869469159474No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC15580.16422420926254055Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15420.16253769620207797No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT14470.15252402490558162Clontech Universal Primer Mix Long (96% over 26bp)
CTCTTAGGGGTTTTGTTTTTCTCTTTTGAGAGAGGCCATCATTATCATTG14170.14936181291721434No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA13420.14145628294629614No Hit
ATACAGAATAGTCAAATATTTTGCACAAGGAAATCTTGTTGATGATCCGT12140.12796417846259578No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT11560.12185056861841903Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT10750.1133125962498274Clontech SMART CDS Primer II A (100% over 26bp)
CCTCCGAGGTCCGGGTCACCAGTCTCTGCTCTTCCCAGCCTCTCCGGCGC10140.10688276520681393No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGCATCTTCTATCGCGTATG9980.1051962521463514No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9890.1042475885498412No Hit
CCGTAGAACTGATGCTTGCAGAACGCAGTCTCTTAGGGGTTTTGTTTTTC9780.10308811082077321No Hit
CTTTTATTGTGAATGGTAACAGCTCGGGGCCTTGAATCTAACCAACAGGT9680.10203404015798412No Hit
ATACACATCTCCGAGCCCACGAGACCGAGCATCTTCTATCTCGTATGCCG9500.10013671296496374No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)