FastQCFastQC Report
Tue 10 May 2022
EGAF00003655723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003655723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences648606
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA45190.6967249763338605Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG27470.4235236800152944Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT23370.3603111904607728Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20440.3151373869498586No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG17320.26703422416690564Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG16150.24899553812329825Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGACGCTTTAATCTCGTATG15690.24190340514888853No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13420.2069052706882144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12840.1979630160683065No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT11760.18131192125882276Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11170.17221548983512333Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT10760.1658942408796712Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA10250.15803122388630386No Hit
ATACACATCTCCGAGCCCACGAGACTAAGACGCTTTAATCTCGTATGCCG9900.1526350357536008No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG9860.15201832853843472No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT9460.1458512563867741Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACAT8710.1342879961024104No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8180.12611662550146005No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8140.125499918286294No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGACGCTTTAATCGCGTATG7190.11085312192609999No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC6980.10761540904647815No Hit
ACACATCTCCGAGCCCACGAGACTAAGACGCTTTAATCTCGTATGCCGTC6880.10607364100856298No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT6870.10591946420477145Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGC6860.10576528740097996No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT6710.1034526353441072No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT6590.10160251369860902No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)