FastQCFastQC Report
Tue 10 May 2022
EGAF00003655731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003655731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1381332
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA73780.5341221371835302Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG39920.28899641795021036Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAGAGTCACTCCTGCCTTCACCATGAAGTCCAGCGGCCTCTT39860.2885620545965778No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT27530.19930038542508247Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26490.19177142062878438No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG25640.18561793978565616Clontech Universal Primer Mix Long (96% over 26bp)
GAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGAATCAAGCTTTCACAG25190.18236021463341182No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG23470.16990846516261116Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT18300.1324808228579371Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTCTGGAAAGTCCTTCAAAGCTGGAGTCTGTCCTCCTAAGAAATCTGCC16630.12039104284849696No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15820.11452713757445711No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC15630.11315165362128729Clontech Universal Primer Mix Long (96% over 26bp)
GTCCAGGGAAGAAGAGATGTTGTCCTGACACTTGTGGCATCAAATGCCTG15110.10938717122313825No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)