FastQCFastQC Report
Tue 10 May 2022
EGAF00003655758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003655758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2885451
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCTGCGGT133100.4612797098269906No Hit
GTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGG97000.3361692851481449No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA92870.32185609805884763No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC88370.3062606157581605No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG85920.2977697420611197No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCATGCCCAG82080.28446159716453334No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT81810.2835258682264921No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG79470.27541621743013484No Hit
TTTCTATTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT69960.2424577648346827No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGTGCCACCTCTG68580.23767515026247193No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA66480.23039725852215132Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTG65420.2267236560246561No Hit
CCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAG63800.22110928239640876No Hit
GTACATGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGG61860.21438589669344585No Hit
GTACATGGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTG61320.21251443881736337No Hit
GTTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGC58680.20336508920096025No Hit
GGGTGTTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCT52870.18322958871940645No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCA50790.17602101023375546No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT50010.17331779330163638No Hit
CGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCACCCTCC49490.17151564868022365No Hit
GAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAG46260.16032155805106377No Hit
GGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCA45950.1592472026036831No Hit
GGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATG45690.15834613029297673No Hit
CCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTT45590.1579995640196281No Hit
TCGCCACACAGGGTGTTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC45200.15664795555356859No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA44210.1532169494474174No Hit
GCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCT43570.15099892529798634No Hit
GCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGG42030.14566180468841786No Hit
GGGTTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAA41740.14465676249570691No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGCAGAGAGCATTCCATCTGG41500.14382500343967025No Hit
GCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCAATAGAAAAGTCCACAA40820.14146835278089975No Hit
TCTCCAGGGTTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTC39150.1356806960159781No Hit
GTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGC37820.13107136458044166No Hit
CTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGG37540.13010097901506557No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37360.12947715972303808No Hit
GCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCTGAGTGAGCACCTG37270.12916525007702434No Hit
GAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTC36200.1254569909521943No Hit
AGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCATGCCC34900.12095162939866247No Hit
GGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAG34480.11949605105059834No Hit
CCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCTGCGGTGT32930.11412427381369497No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG32920.11408961718636013Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTA32340.11207953280093821No Hit
GCAAAAGACCCTTTGCCCGGGGCGTTTCCTTTCCCGTTGGGGCTTCCACT32230.11169830990025477No Hit
GTCTTGAGGCTCTCAATAGAAAAGTCCACAACTTCCAGATGGAATGCTCT32140.11138640025424101No Hit
GATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGC31610.10954959900549342No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30400.10535614709797532Clontech Universal Primer Mix Long (96% over 26bp)
GTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGT29460.10209842412849846No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)