FastQCFastQC Report
Tue 10 May 2022
EGAF00003655793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003655793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674837
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGG46310.6862397882747983No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA44570.6604557841375028Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGG42600.6312635495682661No Hit
CCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAG27900.4134331697876672No Hit
GGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCC25630.37979541726372446No Hit
GGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGA24530.3634951847631354No Hit
ATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCA23500.3482322397853111No Hit
GTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGC22970.34037849139866366No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT22260.3298574322391926Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG20180.29903517441989697Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCATC19040.2821422061920138No Hit
GTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCT18570.27517756139630756No Hit
GCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTT16370.2425770963951295No Hit
TAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCT16300.24153980887236473No Hit
AGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAG15710.23279695689477609No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG15030.22272044953077558Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCA13720.20330835446189227No Hit
GCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTC13430.1990110204390097No Hit
GGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCA13210.1957509739388919No Hit
CTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTC12820.189971800597774No Hit
ACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCT12560.18611901837036202No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG12300.18226623614295007Clontech Universal Primer Mix Long (96% over 26bp)
CACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTT11530.17085607339253775No Hit
AAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAA11130.1649287161195963No Hit
GTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGAGGGTG10840.1606313820967137No Hit
GTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGAGGGAG10810.16018683030124312No Hit
GACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGC10750.1592977267103019No Hit
ATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGT10740.15914954277847837No Hit
GAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAG10710.15870499098300775No Hit
GAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTG10640.157667703460243No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10570.15663041593747823No Hit
GGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAG10500.15559312841471348No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTCTACGGCGATCTCGTATG9160.13573648155035956No Hit
TGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGC8750.12966094034559458No Hit
GGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGA8410.12462268666359431No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT8380.12417813486812371Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTAAATGCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTT8310.12314084734535895No Hit
CCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTCTCTGCTGC8260.12239992768624126No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC8120.12032535264071176Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT8040.11913988118612347Clontech Universal Primer Mix Long (96% over 26bp)
GAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTC7830.1160280186178292No Hit
GCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCA7630.11306433998135845No Hit
GGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATC7620.11291615604953492No Hit
GCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATG7570.11217523639041724No Hit
GGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGAGGGTGTGTCCATGAG7390.10950792561759358No Hit
GTACATGGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGG7120.1055069594583581No Hit
TAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTCTTC7000.10372875227647567No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)