FastQCFastQC Report
Tue 10 May 2022
EGAF00003655795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003655795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1646086
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGG139660.8484368374434871No Hit
TTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGG131150.796738445014416No Hit
CCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAG103310.6276099790654923No Hit
GGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCC86510.5255496978894176No Hit
GGACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCATC77260.46935579307520997No Hit
GGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGA76040.46194427265647114No Hit
GTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGC72620.441167715417056No Hit
ATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCA70840.43035418562578137No Hit
GTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCT60310.3663842593886346No Hit
GGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCA55010.3341866706842777No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA49750.3022320826493877Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTT46720.28382478193727423No Hit
GCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTC45220.2747122568322676No Hit
AGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAG43780.2659642327314612No Hit
TAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCT43560.2646277290493935No Hit
GAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAG41880.25442170093178607No Hit
ACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCT39420.23947715975957515No Hit
CTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTC39120.2376546547385738No Hit
GTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGAGGGAG38940.236561151725973No Hit
GAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTG37760.2293926319767011No Hit
AAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAA37490.22775237745779991No Hit
AAACCCTCCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTT36680.2228316139010963No Hit
GGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGA36080.2191866038590936No Hit
GTACATGGGACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCA35750.2171818483359922No Hit
CACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTT34700.2108030807624875No Hit
GTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGAGGGTG33540.20375606134794902No Hit
ATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGT32580.19792404528074475No Hit
CATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAG31850.19348928306297486No Hit
GACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGC31430.19093777603357298No Hit
TGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGC30840.187353516158937No Hit
CCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTCTCTGCTGC28530.17332022749722675No Hit
CTCAGAGACGGAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATG28250.17161922281095884No Hit
GGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATC28170.17113322147202517No Hit
TCTAAATGCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTT27740.1685209642752566No Hit
TTTGTGATGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGAC27170.16505820473535404No Hit
TTTGCGATGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGAC27040.1642684525595868No Hit
CTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGA26300.15977294017445018No Hit
GCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCA26250.15946918933761664No Hit
GAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTC25970.15776818465134873No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24780.1505389147347101No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG24620.14956691205684272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTT24490.14877715988107548No Hit
TCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACT23380.14203389130337055No Hit
GATCAAGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGCTGGTGGACAC23270.1413656394623367No Hit
GTACATGGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGG23020.13984688527816894No Hit
GGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAG23010.13978613511080223No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT22870.13893563276766827Clontech Universal Primer Mix Long (96% over 26bp)
CTGTCACCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCT22830.13869263209820143No Hit
CCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACAT22590.13723462808140036No Hit
GGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGAGGGTGTGTCCATGAG22470.13650562607299982No Hit
TTTCAGCCCTGATCAAGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGC21770.13225311435733003No Hit
GCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGCTTTT21250.12909410565426108No Hit
AATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTT20820.1264818484574925No Hit
GCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAAT20710.1258135966164587No Hit
GCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATG20540.1247808437712246No Hit
CTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCA20190.1226545879133897No Hit
GTACATGGGAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAG19740.1199208303818877No Hit
GTATCAACGCAGAGTACATGGGATACTAGCCCACCAGACTCAGAGACGGA19040.11566831866621793No Hit
ACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTC18940.11506081699255082No Hit
GATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTCATGGACACAC18930.1150000668251841No Hit
GTGTCCACCAGCTTCTTCAGCTGAGCCCCTGCCTCCCTCATGTCTTGATC18810.11427106481678358No Hit
CCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCC18340.11141580695054815No Hit
GATCTGCCCGAGCTTTCAGCGTGTCATCGAAACTCTCCTCATGGACACAC18280.11105130594634789No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG18230.11074755510951431Clontech Universal Primer Mix Long (96% over 26bp)
GGGGGAGGGTGTCCACCAGCTTCTTCAGCTGAGCCCCTGCCTCCCTCATG18220.11068680494214762No Hit
CAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTGG17620.10704179490014494No Hit
GACTCAGAGACGGAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCA17620.10704179490014494No Hit
CTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGC17460.10606979222227758No Hit
TAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTCTTC17420.10582679155281072No Hit
ATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCATC17420.10582679155281072No Hit
GGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGC17410.105766041385444No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG17330.10528004004651034Clontech Universal Primer Mix Long (96% over 26bp)
CTCATGTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGA17120.1040042865318094No Hit
CATGGGACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAG16790.10199953100870794No Hit
GAGTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGG16490.1001770259877066No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)