FastQCFastQC Report
Tue 10 May 2022
EGAF00003655831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003655831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219771
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA97574.439621242111107Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT46972.137224656574343Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG45652.077162136951645Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG36481.659909633209113Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG29591.3464014815421506Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTGTTTATTGCCCATCTCGTATG26921.224911385032602No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21700.9873914210701138No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC21300.9691906575480842Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA20160.9173184815102993No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT18980.8636262291203116Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT17410.7921882322963447Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTGTTTATTGCCCATCGCGTATG16080.7316706935855959No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC14240.6479471813842591Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT13820.6288363796861278Clontech SMART CDS Primer II A (100% over 26bp)
ATACACATCTCCGAGCCCACGAGACTGTTTATTGCCCATCTCGTATGCCG13630.6201910170131637No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12760.580604356352749No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC12480.5678638218873282Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT10510.47822506154133165Clontech SMART CDS Primer II A (100% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC9370.4263528855035469Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8910.40542200745321266No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA8880.40405695018906046No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA8590.39086139663558883Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT8360.3803959576104218Clontech Universal Primer Mix Long (100% over 23bp)
ATACACATCTCCGAGCCCACGAGACTGTTTATTGCCCATCGCGTATGCCG7770.3535498314154279No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT7770.3535498314154279Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA7540.3430843923902608No Hit
ACACATCTCCGAGCCCACGAGACTGTTTATTGCCCATCTCGTATGCCGTC7530.34262937330221005No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG7480.3403542778619563Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA7330.33352899154119514No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG7310.3326189533650937Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA7180.32670370522043396Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG6860.3121430944028102Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC6820.3103230180506072Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG6710.30531780808204906Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC6330.28802708273612077Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG6100.27756164371095365Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT5950.27073635739019253Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG5820.26482110924553287Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC5710.2598158992769747Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5570.25344563204426424Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC5470.2488954411637568Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA5440.24753038389960458No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA5160.23478984943418377No Hit
ACACATCTCCGAGCCCACGAGACTGTTTATTGCCCATCGCGTATGCCGTC4920.22386939132096592No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT4810.21886418135240773Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC4800.218409162264357Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA4670.2124939141196973Clontech Universal Primer Mix Long (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4600.2093087805033421No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT4500.2047585896228347Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA4470.20339353235868246No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA4330.19702326512597204Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT4200.19110801698131238Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC4170.18974295971716015Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAA4120.18746786427690643No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA4080.18564778792470343Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT3960.1801875588680945Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA3950.17973253978004378No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG3930.17882250160394228No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAA3820.1738172916353841No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA3710.16881208166682593No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT3570.1624418144341155Clontech Universal Primer Mix Long (96% over 25bp)
ACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA3380.15379645176115136No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA3360.1528864135850499No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3280.14924626088064394No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC3260.14833622270454247Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAAAAAAAAAAAAA3120.14196595547183205No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC3090.14060089820767982Clontech Universal Primer Mix Long (96% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA2970.13514066915107087Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2960.13468565006302013No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT2830.12877040191836048No Hit
GGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2730.12422021103785302No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA2690.12240013468565007No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA2680.12194511559759932No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA2670.12149009650954859No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2610.11875998198124411No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT2590.11784994380514263No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG2580.11739492471709188Clontech Universal Primer Mix Long (96% over 26bp)
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA2570.11693990562904114Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAA2460.11193469566048296No Hit
CATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2450.11147967657243223No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT2430.11056963839633073No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA2380.108294542956077Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT2370.10783952386802627Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA2350.10692948569192477No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA2340.10647446660387402Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACTCAAAAAAAAAAAAAAAAAAAAAAA2340.10647446660387402No Hit
CACATCTCCGAGCCCACGAGACTGTTTATTGCCCATCTCGTATGCCGTCT2320.10556442842777254RNA PCR Primer, Index 20 (95% over 21bp)
GTATAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2300.10465439025167105Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG2300.10465439025167105Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT2210.10055921845921437Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2200.10010419937116362No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)