FastQCFastQC Report
Tue 10 May 2022
EGAF00003655919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003655919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37967
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10572.783996628651197Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG5811.5302762925698634Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG4271.1246608897200199Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC3030.798061474438328Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2540.6690020280770143Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1980.5215055179497985Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC1870.4925329891748097No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1800.4740959254089077No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1790.471462059156636Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1600.42141860036347356No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA1550.408249269102115No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1320.3476703452998657Clontech Universal Primer Mix Long (100% over 23bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG1110.2923591540021598Clontech Universal Primer Mix Long (96% over 26bp)
GGCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATA1040.2739220902362578No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG1000.26338662522717093No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC970.25548502647035587No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG900.23704796270445386Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC860.22651249769536702No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG850.2238786314430953Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG840.2212447651908236Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG830.21861089893855187Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA810.2133431664340085No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA810.2133431664340085No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG810.2133431664340085No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA800.21070930018173678Clontech Universal Primer Mix Long (96% over 26bp)
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG790.20807543392946506No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC780.20544156767719335Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCA760.20017383517264994No Hit
ACACACGATAGTGATGTAGAGATCTTAAATCCTGATCTTCATATCGCGAC730.1922722364158348No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG730.1922722364158348No Hit
CTCTTAGCATAGTGTCCGCTCATACATTAGATGGACGGCTCATTAGTATC660.17383517264993284No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG650.17120130639766112Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA630.16593357389311772Clontech Universal Primer Mix Long (95% over 21bp)
GCGTGACGCAGTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCA620.163299707640846No Hit
TTTATGAACTAGCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTT620.163299707640846No Hit
ATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGATCACG610.1606658413885743No Hit
GCACAAAGCTTGTTCCGTTAGCTGGCATAAGATTCCATGCCTAGATGTGA590.15539810888403086No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.15539810888403086No Hit
GTGTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCG590.15539810888403086No Hit
GCCCGATCCCGGAAGATACGCTCTAAGCTCGGCAATCGCTCTTGCCGTGC580.15276424263175914No Hit
CAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGAT560.14749651012721576No Hit
GTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTAT560.14749651012721576No Hit
TTGATGTACAGGGTGAAGGAACTGTAACGGCAGCTGATATTACACACGAT560.14749651012721576No Hit
GTATAAGGATGCTCTGTGAACTGTCATCGGTCCGATCAATTAGTCTAGTG560.14749651012721576No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG550.14486264387494405No Hit
TGTATAGACAGTGCGCGTATCATATAGGATGCGGCGATCTCATACAGCGT550.14486264387494405No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550.14486264387494405No Hit
GAATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGT540.14222877762267233No Hit
CCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTCC530.13959491137040062No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA530.13959491137040062No Hit
GATCCGGCGCGATCCGGATTTCGTGCCCGATCCGCTGATCACCGACTACC510.13432717886585718No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT510.13432717886585718No Hit
GCTTAGAGCGTATCTTCCGGGATCGGGCAAAGGGGCAACTCGAGCTAATC510.13432717886585718No Hit
GGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATA500.13169331261358547No Hit
CTCGTGGAAGAGCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTC500.13169331261358547No Hit
AGGCTACCCGGATCGATGACGCGAATTGGGGACATTCAAATGAGCATCCT490.12905944636131375No Hit
AGCTTGTTCCGTTAGCTGGCATAAGATTCCATGCCTAGATGTGATACACG480.12642558010904206No Hit
CCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATCTTTTCCCTATCT480.12642558010904206No Hit
GCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCTC480.12642558010904206No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA480.12642558010904206No Hit
CTATAGTCATCGTCCATAACACGATCTAGTGAAACCTTCAAGCATGAATT480.12642558010904206No Hit
ACCAGAGACTTTGTGCTGTCGCCTAAGGGACTATCACCCTCTACGGTCGC460.12115784760449864No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC460.12115784760449864Clontech SMART CDS Primer II A (96% over 26bp)
CTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTGATCCCTTCCG460.12115784760449864No Hit
ATTCAAATGAGCATCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGA460.12115784760449864No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT460.12115784760449864Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTC460.12115784760449864No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA460.12115784760449864Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTGCAG460.12115784760449864No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT450.11852398135222693No Hit
ATACATAAGTGTATAATGTCTCGGACCCGTCGCAACGGTTGTTAATATGA450.11852398135222693No Hit
CAGAGGTCCCGCCGTACGCTGAGGACCACCTGAAACGGGCATCGTCGCTC450.11852398135222693No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT440.11589011509995523Clontech Universal Primer Mix Long (96% over 25bp)
ATCATATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAG440.11589011509995523No Hit
GTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC440.11589011509995523No Hit
AACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTG440.11589011509995523No Hit
GAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCAGC440.11589011509995523No Hit
TCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCA430.11325624884768351No Hit
CGATATGACAAGTTAACAAGACGGCGCAGGTTGATGCTTCCAATAAACAT430.11325624884768351No Hit
AGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGGTACC430.11325624884768351No Hit
GAGACATAACGATAGCGACTCTGTCTCAGGGGATCTGCATATGTTTGCAG430.11325624884768351No Hit
GGCTTACGACACGACTAAGTGCTTGCCGACGTTACTAATGTGTCAGCGGC420.1106223825954118No Hit
AATTGGGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACCGCCGCC420.1106223825954118No Hit
GAATAGGTACCAAGAAAAAAACGAACCTTTGGGTTCCAGAGCTGTACGGT410.1079885163431401No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA410.1079885163431401No Hit
GTATCACGATGTGCGCGTGGCGCTGCCCCGTCGAACGATCAGCCAGGGTG410.1079885163431401No Hit
CTGTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGC410.1079885163431401No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA410.1079885163431401Clontech Universal Primer Mix Long (96% over 26bp)
ACTAATGTGTCAGCGGCCCAACTGTAATTGATCCACACTGACGAATCATG400.10535465009086839No Hit
TTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGTGA400.10535465009086839No Hit
AAATAGCGCTGAATCGCTTCTTTAAAGGCTGCAGCGTCGTCCTCAAATTT400.10535465009086839No Hit
TAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCC400.10535465009086839No Hit
CTGCAAACATATGCAGATCCCCTGAGACAGAGTCGCTATCGTTATGTCTC400.10535465009086839No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC390.10272078383859667No Hit
CAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACTGATTGCCGAGCAA390.10272078383859667No Hit
GATATAGGTCGTGGCTCCCTGTATATCGAGGTGAGTCTATCTGGATCAAC390.10272078383859667No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT390.10272078383859667No Hit
CAGTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGAT380.10008691758632497No Hit
GCGTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGTGTG380.10008691758632497No Hit
ACAGCAATTGAAGTGAAGATGATTGACCGCACGCAATTGATATTAGATAT380.10008691758632497No Hit
CGCCCTTCACCACGCCGGGGCCCAGGCCCAGGTCGCACGCTTCAAGCGAG380.10008691758632497No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)