FastQCFastQC Report
Tue 10 May 2022
EGAF00003655930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003655930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225476
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG106974.744185633947738No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA97624.329507353332505Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG51742.2947009881317744Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT49682.203338714541681Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG34371.5243307491706433Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG30741.3633380049317887Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC21890.9708350334403661Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT19470.8635065372811297Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT19090.8466533023470347Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19030.8439922652521777No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA18670.828026042683035No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC15450.6852170519257039Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT15230.6754599159112278Clontech SMART CDS Primer II A (100% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13350.5920807536057053No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC12440.5517216910003725Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT11640.5162411964022778Clontech SMART CDS Primer II A (100% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA9500.4213308733523745Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT9390.41645230534513644Clontech Universal Primer Mix Long (100% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8880.3938334900388511No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT8740.3876244034841846Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG8330.369440650002661Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC8190.36323156344799445Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG8170.3623445510830421Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA7460.3308556121272331No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG7330.32509003175504264Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA7210.3197679575653285No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC7110.31533289574056667Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA6980.30956731536837623No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG6720.29803615462399546Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG6690.2967056360765669Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA6370.282513438237329Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC6120.2714257836754244Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC6060.2687647465805673Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT5770.255903067288758Clontech SMART CDS Primer II A (100% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC5670.25146800546399617Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC5560.24658943745675815Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG5390.239049832354663Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT4970.22042257269066332Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4800.21288296758856817No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4790.21243946140609202Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA4530.20090830066171123Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA4480.1986907697493303No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA4390.19469921410704466Clontech Universal Primer Mix Long (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG4140.18361155954514005No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA4110.1822810409977115No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT4080.18095052245028295Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT4000.1774024729904735Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA4000.1774024729904735No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA3930.1742979297131402No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA3830.16986286788837837Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT3800.16853234934094982Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA3650.16187975660380705No Hit
GTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAA3650.16187975660380705No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC3580.1587752133264738Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA3400.15079210204190246No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAA3360.14901807731199773No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC3060.1357128918377122Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAAAAAAAAAAAAA3040.13482587947275987No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2940.130390817647998No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT2860.12684276818818854Clontech Universal Primer Mix Long (96% over 25bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT2790.12373822491085526No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA2760.1224077063634267No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC2710.12019017545104578Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG2680.11885965690361724Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA2670.11841615072114105Clontech SMART CDS Primer II A (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT2610.11575511362628395Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2580.11442459507885538No Hit
GGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2550.11309407653142685No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT2520.1117635579839983No Hit
GTATAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2480.10998953325409355Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA2480.10998953325409355No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA2460.1091025208891412Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA2450.10865901470666502No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA2420.10732849615923647Clontech Universal Primer Mix Long (95% over 22bp)
GCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG2380.10555447142933172Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA2350.10422395288190317No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA2260.10023239723961752Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)