FastQCFastQC Report
Tue 10 May 2022
EGAF00003656507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003656507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104276
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCCTTTATTTAATCTCGTATG6590.6319766772795274No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3820.3663354942652192No Hit
ATACACATCTCCGAGCCCACGAGACTCCCTTTATTTAATCTCGTATGCCG3740.3586635467413403No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCCTTTATTTAATCGCGTATG2990.28673903870497525No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2920.2800260846215812No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2570.24646131420461084No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2490.2387893666807319No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2310.2215274847520043Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACATCTCCGAGCCCACGAGACTCCCTTTATTTAATCTCGTATGCCGTC2020.19371667497794315No Hit
ATACACATCTCCGAGCCCACGAGACTCCCTTTATTTAATCGCGTATGCCG1730.165905865203882No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA1640.15727492423951822No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.14960297671563927No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG1520.1457670029536998No Hit
ACACATCTCCGAGCCCACGAGACTCCCTTTATTTAATCGCGTATGCCGTC1430.137136061989336No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1390.13330008822739653Clontech Universal Primer Mix Long (96% over 26bp)
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA1360.13042310790594192No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT1330.12754612758448733No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC1310.12562814070351758No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1130.10836625877478998No Hit
GTACATGGGAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCC1120.10740726533430513No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT1110.10644827189382024Clontech SMART CDS Primer II A (100% over 26bp)
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC1060.10165330469139591No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1050.10069431125091105No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)