FastQCFastQC Report
Tue 10 May 2022
EGAF00003656511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003656511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99405
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4190.4215079724359942Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACCCACGGCACAGTATCTCGTATG4180.42050198682158846No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4160.418490015592777No Hit
CTTATACACATCTCCGAGCCCACGAGACCCACGGCACAGTATCGCGTATG3190.32090941099542275No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA3020.30380765555052563No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2390.24043056184296563Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2350.2364066193853428No Hit
ATACACATCTCCGAGCCCACGAGACCCACGGCACAGTATCTCGTATGCCG2130.21427493586841706No Hit
ATACACATCTCCGAGCCCACGAGACCCACGGCACAGTATCGCGTATGCCG2080.2092450077963885No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2070.2082390221819828No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1960.19717318042351992Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1620.16296966953372566No Hit
ACACATCTCCGAGCCCACGAGACCCACGGCACAGTATCGCGTATGCCGTC1570.1579397414616971No Hit
ACACATCTCCGAGCCCACGAGACCCACGGCACAGTATCTCGTATGCCGTC1500.1508978421608571No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA1400.14083798601679995No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1370.13782002917358283Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1200.12071827372868568Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1070.1076404607414114Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1050.10562848951259998No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT1010.10160454705497712Clontech SMART CDS Primer II A (100% over 26bp)
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA1010.10160454705497712No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1000.1005985614405714No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)