FastQCFastQC Report
Tue 10 May 2022
EGAF00003656531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003656531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342772
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAACGGTCTTCCATCTCGTATG7600.22172172756234465No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4600.13419999299826124No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4310.12573955865706649No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4260.12428086308099845No Hit
CTTATACACATCTCCGAGCCCACGAGACGAACGGTCTTCCATCGCGTATG4180.12194695015928957No Hit
ATACACATCTCCGAGCCCACGAGACGAACGGTCTTCCATCTCGTATGCCG4030.11757086343108537No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3580.10444260324647288Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3520.1026921685551912No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)