FastQCFastQC Report
Tue 10 May 2022
EGAF00003656555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003656555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89347
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCTGAACCTTATCTCGTATG5950.6659428967956394No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4740.5305158539178708No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4420.4947004376196179Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA3900.436500386134957No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCTGAACCTTATCGCGTATG3570.39956573807738366No Hit
ATACACATCTCCGAGCCCACGAGACTTTCTGAACCTTATCTCGTATGCCG3510.39285034752146125No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3080.34472338187068396No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2840.3178618196469943No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2550.28540409862670263Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2540.28428486686738225Clontech Universal Primer Mix Long (96% over 26bp)
ACACATCTCCGAGCCCACGAGACTTTCTGAACCTTATCTCGTATGCCGTC2440.2730925492741782No Hit
ATACACATCTCCGAGCCCACGAGACTTTCTGAACCTTATCGCGTATGCCG2300.25742330464369256No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2070.23168097417932332No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA1840.20593864371495404No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT1720.19250786260310923No Hit
ACACATCTCCGAGCCCACGAGACTTTCTGAACCTTATCGCGTATGCCGTC1670.1869117038065072No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1640.183554008528546Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1600.1790770814912644No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1550.17348092269466237Clontech Universal Primer Mix Long (96% over 26bp)
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG1480.16564630037941955No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT1450.16228860510145837Clontech SMART CDS Primer II A (100% over 26bp)
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC1440.16116937334213796No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT1380.15445398278621555No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT1360.15221551926757473No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1320.14773859223029312No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1290.14438089695233192No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT1200.1343078111184483Clontech SMART CDS Primer II A (100% over 26bp)
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1170.13095011584048707No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA1160.1298308840811667No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC1110.12423472528456467No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT1090.12199626176592386No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAA1070.11975779824728308No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1060.11863856648796267No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC1050.11751933472864226No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA960.10744624889475864No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC950.10632701713543824No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT930.10408855361679742Clontech Universal Primer Mix Long (96% over 26bp)
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG920.10296932185747702No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT910.10185009009815664Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC910.10185009009815664Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCC900.10073085833883623No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)